Regulation of malate dehydrogenase from an osmoconformer by salt

Sarkissian, Igor V.; Gomolinski, Eva

doi:10.1016/0305-0491(76)90034-1

Cited by 4 publications

(12 citation statements)

References 11 publications

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“…It has several effects on the mitochondrial malate dehydrogenase from beef heart, including decreasing Vmax, and increasing Km values for NADH and OAA, and slightly increasing the K; for NADH (Bracht and deCampello, 1979). While the beef heart mMDH exhibits a Vmax effect, little Vmax effect is observed for the MDH from the oyster when the strength of the phosphate assay buffer is increased; however, increased phosphate buffer strength does have a competitive effect by increasing the K^'s for oxaloacetate and NADH (Sarkissian and Gomolinski, 1976). As with the mMDH and cMDH from the gill tissue of the ribbed mussel, mammalian mMDH appears to be more sensitive to inhibition by chloride derivatives than the cMDH.…”

Section: Resultsmentioning

confidence: 99%

“…Increases in Mg2+ in addition to Ca2+ and Na+ are observed when the ionic concentration of the medium increases (Shumway, 1977b). The increased ionic concentration of hemolymph may then cause an increase in the intracellular ionic composistion in tisssues (Sarkissian and Gomolinski, 1976). Therefore, some regulation of MDH activity by Mg2+ and possibly CI" may occur in the ribbed mussel in response to changing environmental conditions.…”

Section: Resultsmentioning

confidence: 99%

“…The increases are attributed to increased concentrations of Na+, Ca2+, and Mg2+ (Shumway, 1977b). That increasing the ionic concentration of the bathing medium also increases the tissue ionic content has been suggested (Sarkissian and Gomolinski, 1976).…”

Section: Hyperosmotic Adjustmentmentioning

confidence: 95%

“…The reduction of oxaloacetate by cMDH, along with the production of opines by the opine dehydrogenases, has been suggested to function in a manner analogous to the mammalian LDH in maintaining the NAD-requiring glyceraldehyde-3phosphate dehydrogenase reaction of glycolysis (Fields, 1983;Hochachka, 1980). cMDH has been described in several mollusc species in various states of purity (oyster: Sarkissian and Gomolinski, 1976;blue mussel: Livingstone, 1976; the gastropod Patella cerulea: Lazou et al, 1987). While the cMDH is polymorphic in many mollusc species, no study has attempted to discern the kinetic differences between allozymes.…”

Section: Hyperosmotic Stressmentioning

confidence: 99%

“…In bivalve molluscs, the cMDH and mMDH are important in the regulation of carbon flux between the cytosol and mitochondria for organic acid (succinate and propionate) biosynthesis during anaerobiosis , for cellular osmolyte (alanine) biosynthesis during hyperosmotic stress (Baginski and Pierce, 1977;Bishop et al, 1981) and for gluconeogenesis (deZwaan et al, 1983a Lazou et al, 1987). Studies with MDHs from other sources indicate that they are susceptible to regulation by pH (Zschoche and Ting, 1973) and ionic strength (Kun et al, 1967;Sarkissian and Gomolinski, 1976;Place and Beynon, 1982). The altered concentration of intracellular metabolites that accumulate under environmental stress may also be important in the regulation of malate dehydrogenase (Kun and Volfin, 1966;Delbruck et al, 1959;Abou-Zamzam and Wallace, 1970;Siegel and Englard, 1962).…”

Section: Illmentioning

confidence: 99%

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Purification and properties of the malic enzyme and the malate dehydrogenases from the gill tissue of the ribbed mussel (Modiolus demissus)

Brodey

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Hyperosmotic Adjustmentmentioning

confidence: 95%

Section: Hyperosmotic Stressmentioning

confidence: 99%

Section: Illmentioning

confidence: 99%

See 3 more Smart Citations

Purification and properties of the malic enzyme and the malate dehydrogenases from the gill tissue of the ribbed mussel (Modiolus demissus)

Brodey

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Role of carbon dioxide fixation, blood aspartate and glutamate in the adaptation of amphibian brain tissues to a hyperosmotic internal environment

Tachiki

Baxter

1980

Neurochem Res

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Mechanisms have been examined by which hyperosmotic blood plasma might elevate the levels of aspartate and glutamate in the brain of the toad Bufo boreas. CO2 fixation was assessed by two in vivo methods using [2-14 C]glucose injected intracisternally. Thirty minutes after injection, the 14C labeling of glutamate and aspartate was more than 100 times greater in brain than in liver. In brain tissues, 40 + % of 14C atoms appeared to be incorporated into aspartate via the pyruvate carboxylase pathway. Brain tissues of control toads and toads adapting or adapted to hyperosmotic plasma osmolality revealed no differences in the rate of CO2 fixation as related to glucose utilization or tissue pool sizes of glutamate and aspartate. Elevated levels of these amino acids in blood plasma preceded increases in brain tissues. Carbon atoms required during hyperosmotic adaptation for expansion of amino acid pools in brain tissues may, in part, originate from amino acids in blood but apparently not from CO2 fixation in brain.

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Capacity for Cellular Osmoregulation Defines Critical Salinity of Marine Invertebrates at Low Salinity

Podbielski

Hiebenthal²,

Hajati³

et al. 2022

Front. Mar. Sci.

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Low-salinity stress can severely affect the fitness of marine organisms. As desalination has been predicted for many coastal areas with ongoing climate change, it is crucial to gain more insight in mechanisms that constrain salinity acclimation ability. Low-salinity induced depletion of the organic osmolyte pool has been suggested to set a critical boundary in osmoconforming marine invertebrates. Whether inorganic ions also play a persistent role during low-salinity acclimation processes is currently inconclusive. We investigated the salinity tolerance of six marine invertebrate species following a four-week acclimation period around their low-salinity tolerance threshold. To obtain complete osmolyte budgets, we quantified organic and inorganic osmolytes and determined fitness proxies. Our experiments corroborated the importance of the organic osmolyte pool during low-salinity acclimation. Methylamines constituted a large portion of the organic osmolyte pool in molluscs, whereas echinoderms exclusively utilized free amino acids. Inorganic osmolytes were involved in long-term cellular osmoregulation in most species, thus are not just modulated with acute salinity stress. The organic osmolyte pool was not depleted at low salinities, whilst fitness was severely impacted. Instead, organic and inorganic osmolytes often stabilized at low-salinity. These findings suggest that low-salinity acclimation capacity cannot be simply predicted from organic osmolyte pool size. Rather, multiple parameters (i.e. osmolyte pools, net growth, water content and survival) are necessary to establish critical salinity ranges. However, a quantitative knowledge of cellular osmolyte systems is key to understand the evolution of euryhalinity and to characterize targets of selection during rapid adaptation to ongoing desalination.

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Regulation of malate dehydrogenase from an osmoconformer by salt

Cited by 4 publications

References 11 publications

Purification and properties of the malic enzyme and the malate dehydrogenases from the gill tissue of the ribbed mussel (Modiolus demissus)

Purification and properties of the malic enzyme and the malate dehydrogenases from the gill tissue of the ribbed mussel (Modiolus demissus)

Role of carbon dioxide fixation, blood aspartate and glutamate in the adaptation of amphibian brain tissues to a hyperosmotic internal environment

Capacity for Cellular Osmoregulation Defines Critical Salinity of Marine Invertebrates at Low Salinity

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