Regulation of Mating and Filamentation Genes by Two Distinct Ste12 Complexes in Saccharomyces cerevisiae

Abstract

The Saccharomyces cerevisiae transcription factor Ste12 controls two distinct developmental programs of mating and filamentation. Ste12 activity is regulated by Fus3 and Kss1 mitogen-activated protein kinases through two Ste12 inhibitors, Dig1 and Dig2.

Dynamic Ubiquitination of the Mitogen-activated Protein Kinase Kinase (MAPKK) Ste7 Determines Mitogen-activated Protein Kinase (MAPK) Specificity

“…Deletion of Kss1 did not alter the ability of ubp3⌬ cells to invade solid agar, in agreement with previous observations (44). In particular, deletion of Kss1 is thought to promote invasive growth by relieving Dig1/ Dig2-mediated inhibition of Ste12 and Tec1, which mediate transcription of genes containing filamentous response elements (43)(44)(45).…”

Distinguishing Direct versus Indirect Transcription Factor-DNA Interactions

“…This is the case for Ste12 and Tec1, TFs involved in two distinct developmental programs: mating and filamentation (Chou et al 2006). Chou and colleagues have shown that during mating-a process induced by treatment with alpha pheromone-promoters of mating genes are bound mostly by Ste12-Dig1-Dig2, but also by the Ste12-Tec1-Dig1 complex, with Ste12 binding DNA directly.…”

“…Chou and colleagues have shown that during mating-a process induced by treatment with alpha pheromone-promoters of mating genes are bound mostly by Ste12-Dig1-Dig2, but also by the Ste12-Tec1-Dig1 complex, with Ste12 binding DNA directly. During filamentation-a program induced by butanol treatmentpromoters of most filamentation genes are bound by the Tec1-Ste12-Dig1 complex, with Tec1 binding DNA directly (Chou et al 2006).…”

“…Dig1 does not have an identifiable DNA binding domain, and a literature search does not reveal any evidence of Dig1 binding DNA directly. It is known, however, that Dig1 binds DNA indirectly as part of TF complexes together with Ste12 and Tec1 (Chou et al 2006). In such cases where a TF does not bind DNA directly, the motifs one would expect to find enriched in a ChIP-chip experiment will correspond to interacting factors (Mbp1; Ste12 or Tec1) rather than the factor that was profiled (Swi6; Dig1).…”

The TEA Transcription Factor Tec1 Links TOR and MAPK Pathways to Coordinate Yeast Development

“…A block of Tec1-degradation during mating not only interferes with down-regulation of CLN1 expression, but also with an efficient G1 arrest (Brückner et al 2004). In addition, Tec1 is stabilized by complex formation with Ste12 by a mechanism that is independent of the CPD, but involves the C-terminal part of Tec1 (Chou et al 2006;Heise et al 2010).…”