Regulation of micropatterned curvature-dependent FA heterogeneity on cytoskeleton tension and nuclear DNA synthesis of malignant breast cancer cells

Wang, Yongtao; Wang, Nana; Chen, Yazhou; Yang, Yingjun

doi:10.1039/d2tb01774a

Cited by 5 publications

(2 citation statements)

References 52 publications

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“…The expression of collagen, fibronectin and laminin demonstrates the formation of adhesion anchorage between cells and extracellular matrix (ECM) [145,146]. The transmembrane adhesion protein, integrin primarily binds collagen and fibronectin to produce adhesion sites for cell attachment, which is beneficial for migration, division and differentiation of stem cells in the process of tissue regeneration [147][148][149][150]. It can also connect a series of intracellular anchor Fig.…”

Section: Advanced Gene Nanocarriers/scaffolds For Gene Therapy In Tis...mentioning

confidence: 99%

Advanced gene nanocarriers/scaffolds in nonviral-mediated delivery system for tissue regeneration and repair

Zhang,

Hou,

Yin

et al. 2024

J Nanobiotechnol

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Tissue regeneration technology has been rapidly developed and widely applied in tissue engineering and repair. Compared with traditional approaches like surgical treatment, the rising gene therapy is able to have a durable effect on tissue regeneration, such as impaired bone regeneration, articular cartilage repair and cancer-resected tissue repair. Gene therapy can also facilitate the production of in situ therapeutic factors, thus minimizing the diffusion or loss of gene complexes and enabling spatiotemporally controlled release of gene products for tissue regeneration. Among different gene delivery vectors and supportive gene-activated matrices, advanced gene/drug nanocarriers attract exceptional attraction due to their tunable physiochemical properties, as well as excellent adaptive performance in gene therapy for tissue regeneration, such as bone, cartilage, blood vessel, nerve and cancer-resected tissue repair. This paper reviews the recent advances on nonviral-mediated gene delivery systems with an emphasis on the important role of advanced nanocarriers in gene therapy and tissue regeneration.

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Section: Advanced Gene Nanocarriers/scaffolds For Gene Therapy In Tis...mentioning

confidence: 99%

Advanced gene nanocarriers/scaffolds in nonviral-mediated delivery system for tissue regeneration and repair

Zhang,

Hou,

Yin

et al. 2024

J Nanobiotechnol

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“…Although tremendous efforts have been concentrated on chiral origin, development, and functions in diseases, little attention has been paid to the influence of intracellular chirality on nuclear mechanotransduction and DNA synthesis. Cells will spread their anisotropic cytoskeleton structures and present diversified morphologies based on microenvironment-induced biased cell nanomechanics, such as differentiated component, polar topography and asymmetrical matrix mechanics. − The application of microfabrication technology provides the real possibility for controlling stable polar cell morphology, including chirality. − Recently, multiple studies have reported that intracellular chirality can regulate some of the cell behaviors, for example, dynamic cytoskeleton chirality, directional epithelial migration, and biased cell left–right asymmetry. − Compared to the influence of intracellular polarity on the kinematic behavior of cells, the effects of intracellular chirality on nuclear mechanotransduction and DNA synthesis remain unclear. Therefore, it is urgently desired to reveal the mechanism of mechanotransduction in chiral cells.…”

Section: Introductionmentioning

confidence: 99%

Chiral Cell Nanomechanics Originated in Clockwise/Counterclockwise Biofunctional Microarrays to Govern the Nuclear Mechanotransduction of Mesenchymal Stem Cells

Wang,

Tong,

Shi

et al. 2023

ACS Appl. Mater. Interfaces

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Cell chirality is extremely important for the evolution of cell morphogenesis to manipulate cell performance due to left−right asymmetry. Although chiral micro-and nanoscale biomaterials have been developed to regulate cell functions, how cell chirality affects cell nanomechanics to command nuclear mechanotransduction was ambiguous. In this study, chiral engineered microcircle arrays were prepared by photosensitive crosslinking synthesis on cell culture plates to control the clockwise/counterclockwise geometric topology of stem cells. Asymmetric focal adhesion and cytoskeleton structures could induce chiral cell nanomechanics measured by atomic force microscopy (AFM) nanoindentation in left-/right-handed stem cells. Cell nanomechanics could be enhanced when the construction of mature focal adhesion and the assembly of actin and myosin cytoskeletons were well organized in chiral engineered stem cells. Curvature angles had a negative effect on cell nanomechanics, while cell chirality did not change cytoskeletal mechanics. The biased cytoskeleton tension would engender different nuclear mechanotransductions by yes-associated protein (YAP) evaluation. The chiral stimuli were delivered into the nuclei to oversee nuclear behaviors. A strong cell modulus could activate high nuclear DNA synthesis activity by mechanotransduction. The results will bring the possibility of understanding the interplay of chiral cell nanomechanics and mechanotransduction in nanomedicines and biomaterials.

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Focal adhesion and actin orientation regulated by cellular geometry determine stem cell differentiation via mechanotransduction

Wang,

Yang,

Wang

et al. 2024

Acta Biomaterialia

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Regulation of micropatterned curvature-dependent FA heterogeneity on cytoskeleton tension and nuclear DNA synthesis of malignant breast cancer cells

Abstract: Breast cancer is considered as a worldwide disease due to the high incidence and malignant metastasis. Although numerous techniques have been developed well to conduct breast cancer therapy, the influence...

Cited by 5 publications

References 52 publications

Advanced gene nanocarriers/scaffolds in nonviral-mediated delivery system for tissue regeneration and repair

Advanced gene nanocarriers/scaffolds in nonviral-mediated delivery system for tissue regeneration and repair

Chiral Cell Nanomechanics Originated in Clockwise/Counterclockwise Biofunctional Microarrays to Govern the Nuclear Mechanotransduction of Mesenchymal Stem Cells

Focal adhesion and actin orientation regulated by cellular geometry determine stem cell differentiation via mechanotransduction

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