Regulation of microRNA-145 by growth arrest and differentiation

Rocca, Gaspare La; Shu, Bin; Audia, Alessandra; Ferrari-Amorotti, Giovanna; Mellert, Hestia; Calabretta, Bruno; McMahon, Steven B.; Sepp‐Lorenzino, Laura; Baserga, Renato

doi:10.1016/j.yexcr.2010.11.010

Cited by 17 publications

(12 citation statements)

References 39 publications

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“…It is shown that overexpressed miR-145 inhibits cell proliferation, invasion, and tumor growth, while promoting cell apoptosis in ovarian cancer. By negatively regulating expressions of its target genes, such as plasminogen activator inhibitor-1 (PAI-1), sex-determining region Y (SRY)-box 2 (Sox-2), IRS-1, c-Myc, and p70S6K1, miR-145 plays a critical role in these cellular processes [29,48,49]. The present study showed miR-145 was significantly down-regulated in ovarian cancer.…”

Section: Discussionmentioning

confidence: 61%

Serum microRNA-145 as a novel biomarker in human ovarian cancer

et al. 2015

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Ovarian cancer is one of the most threatening diseases among women in the world. Current detection methods are expensive and lack accuracy. Thus, a fast, non-invasive biomarker for detecting ovarian cancer is urgently needed. Compelling evidences have been demonstrated that microRNAs, a large family of single-stranded and non-protein-coding RNA molecules, can serve as useful biomarkers in cancer detection. In this study, the relative expressions of microRNA-145 (miR-145) in the serum of patients with ovarian cancer and healthy controls were investigated in an independent study. Subsequently, the diagnosis and prognosis value of miR-145 as a biomarker for ovarian cancer were examined. Furthermore, we performed a meta-analysis to summarize all the results from published studies and this study. Relative expressions of miR-145 were investigated in three independent groups (malignant ovarian cancer, benign ovarian tumor, and healthy controls), comprising a total of 270 participants. Receiver operating characteristic (ROC) curves and overall survival (OS) curves were conducted to compare miR-145 level and clinical characteristics among the three groups. The results showed that relative expressions of the serum miR-145 were significantly down-regulated in patients with malignant ovarian cancer and benign ovarian cancer, compared to healthy controls (P < 0.01). Serum miR-145 levels could discriminate patients with malignant ovarian cancer from healthy controls, with a power area under the curve (AUC) of 0.82 (95 % confidence interval (CI) = 0.77-0.88). Furthermore, patients with low serum levels of miR-145 had a significantly shorter median overall survival rate (hazard ratio (HR) = 1.81, 95 % CI = 1.03-3.17, P = 0.039). The meta-analysis yields good diagnostic performances of miR-145 in various cancers, with an AUC of 0.82 (95 % CI, 0.78-0.85). In conclusion, the present study suggested that miR-145 can potentially serve as an outstanding biomarker for ovarian and other human cancers detection.

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Section: Discussionmentioning

confidence: 61%

Serum microRNA-145 as a novel biomarker in human ovarian cancer

et al. 2015

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“…Another microRNA related to cell differentiation and proliferation is miR-145. La Rocca et al [22] demonstrated that miR-145 expression is increased during cell differentiation. A 2009 study conducted on cell cultures of pre-adipocytes and differentiating adipocytes of obese individuals demonstrated that the expression of miR-145 was greatly reduced during cell differentiation [23].…”

Section: Discussionmentioning

confidence: 99%

“…[20] have suggested that miR-124a is involved in pancreatic cells, acting as a possible regulator of glucose in blood together with miR-375. In addition, it was demonstrated that both miR-143 and miR-145 are involved in cell differentiation [21], [22]. The microRNAs miR-143, miR-145, miR-27a and miR-27b were selected for inclusion in this study because, with the use of bioinformatics tool TargetScan (http://www.targetscan.org/), we verified that miR-27a and miR-27b had the genes of LEP , IGF1 and IL10 as targets.…”

Section: Introductionmentioning

confidence: 99%

The Expression of LEP, LEPR, IGF1 and IL10 in Obesity and the Relationship with microRNAs

et al. 2014

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Obesity is a multifactorial disease, with epigenetic alterations. Have been described modifications in the expression of some microRNAs, and some proteins related to obesity. The objective was to determine and correlate, in obese patients, the gene expression of LEP, LEPR, IGF1, IL10 and of miR-27a, miR-27b, miR-143 and miR-145. RNA was extracted from biopsies of subcutaneous fat, liver and visceral fat of 15 obese subjects submitted to bariatric surgery and of 15 non-obese subjects submitted to cholecystectomy for cDNA synthesis and for RT-PCR. The microRNAs were chosen using the TargetScan software. An increased expression of LEP and IGF1 was detected in the subcutaneous fat of the obese group compared to control, while the expression of IGF1 was higher in the control group than in the obese one. MiRNA-27a had a higher expression in the omentum of the obese patients and there was also a correlation in the expression of miRNA-145 and LEPR in the omentum of this group.

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“…Recent studies have revealed that miR-145 is involved in the progression of various tumors by regulating the expression of multiple target genes, especially some genes which regulate proliferation and differentiation [14,[16][17][18]. However, the role of miR-145 on genesis and development of tumors as well as its multiple target genes are yet to be illuminated.…”

Section: Discussionmentioning

confidence: 99%

“…[13][14][15][16]. It has been proved that miR-145 affects the proliferation, differentiation, and apoptosis of cells by targeting c-Myc, IRS-1, BNIP3, and pluripotency factors including OCT4, SOX2, and KLF4 [14,[16][17][18]. Nevertheless, the role and probable pathway of miR-145 in osteosarcoma carcinogenesis, especially the effect in invasion and metastasis of cancer cells, still need further study.…”

Section: Introductionmentioning

confidence: 99%