Regulation of Microtubule Dynamics in Migrating Cells: A New Role for Rho GTPases

Wittmann, Torsten; Waterman‐Storer, Clare M.

doi:10.1002/0470011742.ch13

Cited by 2 publications

(2 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These proteins have been shown to regulate various cellular processes [6–10]. In this current study, a possible additional role for microtubules during Yersinia uptake was investigated given the recent observations that co‐ordination of signals between the actin and microtubule cytoskeletons is mediated through Rho family GTPases during cell locomotion [11–13]. Invasion of cultured cells by certain pathogenic bacteria is known to be significantly affected by drug‐induced disruption of the microtubule system [14–17].…”

Section: Introductionmentioning

confidence: 98%

Microtubule‐dependent regulation of Rho GTPases during internalisation of Yersinia pseudotuberculosis

McGee¹,

Holmfeldt²,

Fällman³

2002

FEBS Letters

View full text Add to dashboard Cite

Internalisation of the human pathogen Yersinia pseudotuberculosis via interaction of bacterial invasin with host L L1 integrins depends on the actin cytoskeleton and involves Src family kinases, focal adhesion kinase, p130Crk-associated substrate, proline-rich tyrosine kinase 2, Rac, Arp 2/3 complex and WASP family members. We show here that Rho GTPases are regulated by the microtubule system during bacterial uptake. Interfering with microtubule organisation using nocodazole or paclitaxel suppressed uptake by HeLa cells. The nocodazole e¡ect on microtubule depolymerisation was partially inhibited through overexpression of Rac, Cdc42, RhoG or RhoA and completely prevented by expression of Vav2. This suggests that microtubules in£uence Rho GTPases during invasin-mediated phagocytosis and in the absence of functional microtubules Vav2 can mimic their e¡ect on one, or more, of the Rho family GTPases. Lastly, overexpression of p50 dynamitin partially inhibited bacterial uptake and this e¡ect was also blocked by coexpression of Vav2, thus further implicating this guanine nucleotide exchange factor in activating Rho GTPases for internalisation during loss of microtubule function.

show abstract

Section: Introductionmentioning

confidence: 98%

Microtubule‐dependent regulation of Rho GTPases during internalisation of Yersinia pseudotuberculosis

McGee¹,

Holmfeldt²,

Fällman³

2002

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…Our findings demonstrate that microtubule polymerization and stability have an important role in lens development and morphogenesis, with the stable population of microtubules linked to proper fiber cell elongation (modeled in Figure 9). As in other systems (44,93), it is the stable population of microtubules that is involved in the directed cell migration of lens fiber cells, as the loss of dynamic microtubules alone did not affect lens fiber cell elongation or the directionality of their migration. The impairment of fiber cell elongation associated with the loss of stabilized microtubules was associated with an increase in F-actin along the interfaces of differentiating lens fiber cells, suggesting that stable microtubules may impact the assembly state of actin.…”

Section: Discussionmentioning

confidence: 54%

Functional role for stable microtubules in lens fiber cell elongation

Logan

Bowen

Menko

2018

Experimental Cell Research

View full text Add to dashboard Cite

The process of tissue morphogenesis, especially for tissues reliant on the establishment of a specific cytoarchitecture for their functionality, depends a balanced interplay between cytoskeletal elements and their interactions with cell adhesion molecules. The microtubule cytoskeleton, which has many roles in the cell, is a determinant of directional cell migration, a process that underlies many aspects of development. We investigated the role of microtubules in development of the lens, a tissue where cell elongation underlies morphogenesis. Our studies with the microtubule depolymerizing agent nocodazole revealed an essential function for the acetylated population of stable microtubules in the elongation of lens fiber cells, which was linked to their regulation of the activation state of myosin. Suppressing myosin activation with the inhibitor blebbistatin could attenuate the loss of acetylated microtubules by nocodazole and rescue the effect of this microtubule depolymerization agent on both fiber cell elongation and lens integrity. Our results also suggest that acetylated microtubules impact lens morphogenesis through their interaction with N-cadherin junctions, with which they specifically associate in the region where lens fiber cell elongate. Disruption of the stable microtubule network increased N-cadherin junctional organization along lateral borders of differentiating lens fiber cells, which was prevented by suppression of myosin activity. These results reveal a role for the stable microtubule population in lens fiber cell elongation, acting in tandem with N-cadherin cell-cell junctions and the actomyosin network, giving insight into the cooperative role these systems play in tissue morphogenesis.

show abstract

Regulation of Microtubule Dynamics in Migrating Cells: A New Role for Rho GTPases

Cited by 2 publications

References 55 publications

Microtubule‐dependent regulation of Rho GTPases during internalisation of Yersinia pseudotuberculosis

Microtubule‐dependent regulation of Rho GTPases during internalisation of Yersinia pseudotuberculosis

Functional role for stable microtubules in lens fiber cell elongation

Contact Info

Product

Resources

About