Regulation of Mild Moxibustion on Uterine Vascular and Prostaglandin Contents in Primary Dysmenorrhea Rat Model

Abstract

Objective. Primary dysmenorrhea (PD) is a common and high incidence disease in gynecology, which seriously affects the quality of life in young women. Our previous study found that mild moxibustion could treat abdominal pain of PD patients, but the mechanism is still unclear. Therefore, this study aims to partly investigate the treatment mechanism of moxibustion for PD, especially on uterine microcirculation. Methods. Forty 3-month-old Sprague Dawley female rats were randomly divided into four groups, including group A (saline control group, n = 10), group B (control plus moxibustion group, n = 10), group C (PD model group, n = 10), group D (PD. model plus moxibustion group, n = 10). The PD rat model was established by injecting estradiol benzoate and oxytocin. Mild moxibustion on Sanyinjiao (SP6) and Guanyuan (CV4) acupoints was once a day, 20 minutes per time, for 10 consecutive days. A vaginal smear was used to test the estrous cycle of rats. Uterine microvascular thickness was observed by stereomicroscope. And we detected the content of prostaglandin F2α (PGF2α) and prostaglandin E2 (PGE2) in uterine tissue by enzyme-linked immunosorbent assay. Results. Mild moxibustion can enlarge the microvessels, improve the microcirculation disturbance, and relieve the swelling of the uterus in PD rats. During the mild moxibustion intervention, the contents of PGF2α and PGE2 in uterus issues were synchronous increases or decreases and the changes of PGE2 were more obvious, but the changes of uterine microvasculature and morphology caused by the decrease of PGF2α were greater than PGE2. Conclusion. Mild moxibustion at SP6 and CV4 acupoints may relax uterine microvascular obstacle by reducing the content of PGF2α in uterine tissue, improve the microcirculation disorder, and then alleviate the PD rat’s uterine swelling.

Moxibustion for Primary Dysmenorrhea: An Adjuvant Therapy for Pain Relief

“…Mild moxibustion [ 64 ] at the Sanyinjiao (SP6) and Guanyuan(CV4) acupoints may relax uterine microvascular obstacles by reducing PGF2 α level in the uterine tissue, improve microcirculation disorder, and alleviate uterine swelling in PD rats. Mild moxibustion can enlarge the microvessels, improve microcirculation disturbance, and relieve uterine swelling in PD rats.…”

Computational systems pharmacology analysis of Tong-Jing-Yi formula in the treatment of dysmenorrhea

“…Excessive release of PG from disintegrating cells during endometrial sloughing causes myometrial hypercontractility, resulting in ischemia and hypoxia of the uterine muscle as well as pain [16,17]. PGs are ubiquitously distributed intracellular substances that are derived from long-chain polyunsaturated fatty acids, such as arachidonic acid, a common component of cell membrane phospholipids [18]. PGs have a range of biological effects on a wide variety of physiological and pathological activities including pain, inflammation, body temperature, and sleep regulation [16].…”

Management of dysmenorrhea through yoga: A narrative review

“…Over the years, a number of hypotheses have been put forth to evaluate the cause of primary dysmenorrhea. Experimental and clinical research has revealed the release of uterine prostaglandins which play a significant role in the progression of primary dysmenorrhea ( 7 ). NSAIDs nonsteroidal anti-inflammatory drugs have shown to be effective in reducing the prostaglandin levels so produced during the bleeding ( 8 ).…”