Regulation of miR-21 expression in human melanoma via UV-ray-induced melanin pigmentation

Lin, Kuan‐Yu; Chen, Chien‐Min; Lu, Cheng‐You; Cheng, Chun-Yuan; Wu, Yung-Chun

doi:10.1002/tox.22421

Cited by 19 publications

(20 citation statements)

References 20 publications

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“…Previous studies have revealed that some non‐coding RNAs, such as microRNAs, in melanocytes can alter and affect the synthesis of melanin or the development of melanoma under UV stimulation . However, the roles of lncRNAs in modulating melanocyte proliferation and melanogenesis are still unclear.…”

Section: The Indispensable Roles Of Lncrnas In Cutaneous Biologymentioning

confidence: 99%

Long non‐coding RNAs in cutaneous biology and proliferative skin diseases: Advances and perspectives

et al. 2019

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Advances in transcriptome sequencing have revealed that the genome fraction largely encodes for thousands of non‐coding RNAs. Long non‐coding RNAs (lncRNAs), which are a class of non–protein‐coding RNAs longer than approximately 200 nucleotides in length, are emerging as key epigenetic regulators of gene expression recently. Intensive studies have characterized their crucial roles in cutaneous biology and diseases. In this review, we address the promotive or suppressive effects of lncRNAs on cutaneous physiological processes. Then, we focus on the pathogenic role of dysfunctional lncRNAs in a variety of proliferative skin diseases. These evidences suggest that lncRNAs have indispensable roles in the processes of skin biology. Additionally, lncRNAs might be promising biomarkers and therapeutic targets for cutaneous disorders.

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Section: The Indispensable Roles Of Lncrnas In Cutaneous Biologymentioning

confidence: 99%

Long non‐coding RNAs in cutaneous biology and proliferative skin diseases: Advances and perspectives

et al. 2019

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“…Experiments to investigate whether melanin transferred to keratinocytes were affected by the presence of purified EVs showed no altered reaction in cocultures of melanocytes and keratinocytes. Interestingly, Lin et al found that miR21 had an inhibitory effect on UV‐stimulated melanogenesis‐regulating proteins in melanoma cells (Lin, Chen, Lu, Cheng, & Wu, ). In the skin, there is a continuous crosstalk between keratinocytes and melanocytes and we also explored whether purified EVs generated from UVA‐irradiated keratinocytes would affect the ability of melanocytes to produce EVs upon UVA exposure.…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles released by melanocytes after UVA irradiation promote intercellular signaling via miR21

Wäster

Eriksson

Vainikka

et al. 2020

Pigment Cell Melanoma Res

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Skin pigmentation is controlled by complex crosstalk between melanocytes and keratinocytes and is primarily induced by exposure to ultraviolet (UV) irradiation. Several aspects of UVA‐induced signaling remain to be explored. In skin cells, UVA induces plasma membrane damage, which is repaired by lysosomal exocytosis followed by instant shedding of extracellular vesicles (EVs) from the plasma membrane. The released EVs are taken up by neighboring cells. To elucidate the intercellular crosstalk induced by UVA irradiation, EVs were purified from UVA‐exposed melanocytes and added to keratinocytes. Transcriptome analysis of the keratinocytes revealed the activation of TGF‐β and IL‐6/STAT3 signaling pathways and subsequent upregulation of microRNA (miR)21. EVs induced phosphorylation of ERK and JNK, reduced protein levels of PDCD4 and PTEN, and augment antiapoptotic signaling. Consequently, keratinocyte proliferation and migration were stimulated and UV‐induced apoptosis was significantly reduced. Interestingly, melanoma cells and melanoma spheroids also generate increased amounts of EVs with capacity to stimulate proliferation and migration upon UVA. In conclusion, we present a novel intercellular crosstalk mediated by UVA‐induced lysosome‐derived EVs leading to the activation of proliferation and antiapoptotic signaling via miR21.

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“…For Western blotting, previously reported procedures were followed 1,15,16 . Basically, protein samples were resolved on polyacrylamide gel electrophoresis (SDS‐PAGE) and thereafter, immunoblotted onto a PVDF membrane.…”

Section: Methodsmentioning

confidence: 99%

Cardioprotective potential of amygdalin against angiotensin II induced cardiac hypertrophy, oxidative stress and inflammatory responses through modulation of Nrf2 and NF‐κB activation

Kung

Badrealam

et al. 2021

Environmental Toxicology

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Heart failure (HF) and cardiac hypertrophy is an unfavorable outcome of pathological cardiac remodeling and represents the most important contributing factor for HF and cardiac hypertrophy. Amygdalin (AMG) is a cyanogenic glycoside derived from bitter almonds. Accumulating evidences have highlighted their pharmacological potentials against various diseases. However, there is no report delineating the potential of AMG against angiotensin (Ang II) induced cardiac injuries. Thus, the present study was performed to explore whether AMG could ameliorate Ang II induced cardiomyopathies and thereby ascertain the underlying mechanisms thereof. To this end, H9c2 cells were treated with Ang II and thereafter treated with various concentration of AMG and finally the cardio‐protective effects of AMG were analyzed through Western blotting, immunofluorescence, and insilico analysis. Our results showed that the cardiomyocyte cell size, inflammatory markers and cytokines(pNF‐κB, TNF‐α, iNOS and COX‐2) were markedly increased following Ang II treatment; nevertheless, treatment with AMG led to considerable decrement in the Ang II induced enlargement of the cardiomyocytes, and attenuate the expression of hypertrophic markers(ANP, BNP and MHC‐7), inflammatory markers and cytokines. Additionally, oxidative stress related proteins (Nrf2, catalase, SOD‐2, and GPX‐4) were markedly increased following AMG treatment. Molecular docking reveals the interaction of AMG with Nrf2 possessing good binding affinity. Cumulatively, our study highlights the cardio‐protective role of AMG against Ang II induced cardiomyopathies, including oxidative stress and inflammation effects. The intriguing in vitro results warrants the need of further animal studies to truly ascertain their potentialities.

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Regulation of miR-21 expression in human melanoma via UV-ray-induced melanin pigmentation

Cited by 19 publications

References 20 publications

Long non‐coding RNAs in cutaneous biology and proliferative skin diseases: Advances and perspectives

Long non‐coding RNAs in cutaneous biology and proliferative skin diseases: Advances and perspectives

Extracellular vesicles released by melanocytes after UVA irradiation promote intercellular signaling via miR21

Cardioprotective potential of amygdalin against angiotensin II induced cardiac hypertrophy, oxidative stress and inflammatory responses through modulation of Nrf2 and NF‐κB activation

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