Regulation of MIR Genes in Response to Abiotic Stress in  Hevea brasiliensis

Gebelin, Virginie; Leclercq, Julie; Hu, Songnian; Tang, Chaorong; Montoro, Pascal

doi:10.3390/ijms141019587

Cited by 27 publications

(15 citation statements)

References 47 publications

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“…Negative co-regulation of miRNA expression and its target is an important step in demonstrating the post-transcriptional control mechanism. For example, in response to saline stress, cleavage of chloroplastic CuZnSOD transcripts was correlated with upregulation of miRNA398 expression in bark and in roots (Gébelin et al, 2013a), but not in leaves. This regulation was not found in relation with the other stresses tested (drought, wounding, flooding, cold, high light intensity (Gébelin et al, 2013a)).…”

Section: Discussionmentioning

confidence: 99%

Post-transcriptional regulation of several biological processes involved in latex production in Hevea brasiliensis

Leclercq

Farinas

et al. 2020

PeerJ

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Background Small RNAs modulate plant gene expression at both the transcriptional and post-transcriptional level, mostly through the induction of either targeted DNA methylation or transcript cleavage, respectively. Small RNA networks are involved in specific plant developmental processes, in signaling pathways triggered by various abiotic stresses and in interactions between the plant and viral and non-viral pathogens. They are also involved in silencing maintenance of transposable elements and endogenous viral elements. Alteration in small RNA production in response to various environmental stresses can affect all the above-mentioned processes. In rubber trees, changes observed in small RNA populations in response to trees affected by tapping panel dryness, in comparison to healthy ones, suggest a shift from a transcriptional to a post-transcriptional regulatory pathway. This is the first attempt to characterise small RNAs involved in post-transcriptional silencing and their target transcripts in Hevea. Methods Genes producing microRNAs (MIR genes) and loci producing trans-activated small interfering RNA (ta-siRNA) were identified in the clone PB 260 re-sequenced genome. Degradome libraries were constructed with a pool of total RNA from six different Hevea tissues in stressed and non-stressed plants. The analysis of cleaved RNA data, associated with genomics and transcriptomics data, led to the identification of transcripts that are affected by 20–22 nt small RNA-mediated post-transcriptional regulation. A detailed analysis was carried out on gene families related to latex production and in response to growth regulators. Results Compared to other tissues, latex cells had a higher proportion of transcript cleavage activity mediated by miRNAs and ta-siRNAs. Post-transcriptional regulation was also observed at each step of the natural rubber biosynthesis pathway. Among the genes involved in the miRNA biogenesis pathway, our analyses showed that all of them are expressed in latex. Using phylogenetic analyses, we show that both the Argonaute and Dicer-like gene families recently underwent expansion. Overall, our study underlines the fact that important biological pathways, including hormonal signalling and rubber biosynthesis, are subject to post-transcriptional silencing in laticifers.

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Section: Discussionmentioning

confidence: 99%

Post-transcriptional regulation of several biological processes involved in latex production in Hevea brasiliensis

Leclercq

Farinas

et al. 2020

PeerJ

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“…Interestingly, these authors showed only that cytosolic HbCuZnSOD genes had no post-transcriptional regulation by microRNA398, which could affect the expression of these transgenes ( Gébelin et al 2012 , Leclercq et al 2012 ). As regards glutathione biosynthesis, the two Hevea genes encoding the glutamyl cysteine ligase are targeted by a microRNA but not the bacterial gene ( EcGSH1 ) used in the experiment ( Gébelin et al 2013 a ). These transgenic plants accumulated three times more glutathione than wild-type plant material ( Martin et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…Superoxide dismutase activity was first reported by d'Auzac et al ( d'Auzac et al 1989 ). This enzyme is encoded by a multigene family consisting of a MnSOD ( Miao and Gaynor 1993 ) and two Cu/Zn SOD s, a cytosolic isoform ( Leclercq et al 2012 ) and a chloroplastic form ( Gébelin et al 2013 a ). The MnSOD gene was first upregulated and then downregulated in latex during the first five tappings of re-opened rubber trees ( Jiyan 2011 ).…”

Section: Ros Production and Scavenging Systems In Laticifersmentioning

confidence: 99%

“…Several ROS-scavenging enzymes have been identified as targets of these microRNAs. The cleavage site by Hbmir398 has been experimentally validated for the chloroplastic CuZnSOD isoform only ( Gébelin et al 2012 ), and regulates the mRNA level of its target gene in response to salinity ( Gébelin et al 2013 a ). The Rboh transcripts have been predicted to be targeted by two miRNAs (HbmiR2914 and HbmiR476) ( Gébelin et al 2012 ).…”

Section: Towards a Comprehensive Analysis Of Redox-related Genes In mentioning

confidence: 99%

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Reactive oxygen species in Hevea brasiliensis latex and relevance to Tapping Panel Dryness

2016

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Environmental stress can lead to oxidative stress resulting from an increase in reactive oxygen species (ROS) and involves redox adjustments. Natural rubber is synthesized in laticifers, which is a non-photosynthetic tissue particularly prone to oxidative stress. This paper reviews the current state of knowledge on the ROS production and ROS-scavenging systems in laticifers. These regulations have been the subject of intense research into a physiological syndrome, called Tapping Panel Dryness (TPD), affecting latex production in Hevea brasiliensis. In order to prevent TPD occurrence, monitoring thiol content appeared to be a crucial factor of latex diagnosis. Thiols, ascorbate and γ-tocotrienol are the major antioxidants in latex. They are involved in membrane protection from ROS and likely have an effect on the quality of raw rubber. Some transcription factors might play a role in the redox regulatory network in Hevea, in particular ethylene response factors, which have been the most intensively studied given the role of ethylene on rubber production. Current challenges for rubber research and development with regard to redox systems will involve improving antioxidant capacity using natural genetic variability.

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“…During latex regeneration, the PPP supplies NADPH for the reducing reactions in rubber biosynthesis, and supplies pentose for nucleic acid synthesis ( Lynen, 1969 ). Various environmental factors such as cold, wind, disease, and especially wounding stresses from tapping affect rubber yield ( Sookmark et al, 2002 ; Venkatachalam et al, 2009 ; Li et al, 2010 ; Gebelin et al, 2013 ; Long et al, 2015 ). The act of tapping the tree inevitably imposes a form of wounding stress that leads to peroxide accumulation in latex.…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of the Glucose-6-Phosphate Dehydrogenase Gene Family in the Para Rubber Tree, Hevea brasiliensis

Long

Fang

et al. 2016

Front. Plant Sci.

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As a key enzyme in the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PDH) provides nicotinamide adenine dinucleotide phosphate (NADPH) and intermediary metabolites for rubber biosynthesis, and plays an important role in plant development and stress responses. In this study, four Hevea brasiliensis (Para rubber tree) G6PDH genes (HbG6PDH1 to 4) were identified and cloned using a genome-wide scanning approach. All four HbG6PDH genes encode functional G6PDH enzymes as shown by heterologous expression in E. coli. Phylogeny analysis and subcellular localization prediction show that HbG6PDH3 is a cytosolic isoform, while the other three genes (HbG6PDH1, 2 and 4) are plastidic isoforms. The subcellular locations of HbG6PDH3 and 4, two latex-abundant isoforms were further verified by transient expression in rice protoplasts. Enzyme activity assay and expression analysis showed HbG6PDH3 and 4 were implicated in PPP during latex regeneration, and to influence rubber production positively in rubber tree. The cytosolic HbG6PDH3 is a predominant isoform in latex, implying a principal role for this isoform in controlling carbon flow and NADPH production in the PPP during latex regeneration. The expression pattern of plastidic HbG6PDH4 correlates well with the degree of tapping panel dryness, a physiological disorder that stops the flow of latex from affected rubber trees. In addition, the four HbG6PDHs responded to temperature and drought stresses in root, bark, and leaves, implicating their roles in maintaining redox balance and defending against oxidative stress.

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Regulation of MIR Genes in Response to Abiotic Stress in Hevea brasiliensis

Cited by 27 publications

References 47 publications

Post-transcriptional regulation of several biological processes involved in latex production in Hevea brasiliensis

Post-transcriptional regulation of several biological processes involved in latex production in Hevea brasiliensis

Reactive oxygen species in Hevea brasiliensis latex and relevance to Tapping Panel Dryness

Identification and Characterization of the Glucose-6-Phosphate Dehydrogenase Gene Family in the Para Rubber Tree, Hevea brasiliensis

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