Regulation of NANOG in cancer cells

Gong, Shuai; Li, Qiuhui; Jeter, Collene; Fan, Qingxia; Tang, Dean G.; Liu, Bigang

doi:10.1002/mc.22340

Cited by 87 publications

(60 citation statements)

References 82 publications

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“…Interestingly, our results on the self-renewal ability of cells through clonal analysis, demonstrated that the capability to form MS is directly correlated to CD133 expression (D283>D341>DAOY), as already reported by other authors [30,46]. This turns out in a significantly higher level of main transcription factors as BMI1, NANOG, and OCT4, all involved in the gene regulatory networks controlling stem cell properties [47][48][49][50]. In agreement with these data, when engrafted in vivo, D283 cells give rise to tumors with high efficiency.…”

Section: Discussionsupporting

confidence: 84%

Human Medulloblastoma Cell Lines: Investigating on Cancer Stem Cell-Like Phenotype

Casciati

Tanori

Manczak

et al. 2020

Cancers

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Medulloblastoma (MB) is the most common malignant pediatric brain tumor. Despite the progress of new treatments, the risk of recurrence, morbidity, and death remains significant and the long-term adverse effects in survivors are substantial. The fraction of cancer stem-like cells (CSCs) because of their self-renewal ability and multi-lineage differentiation potential is critical for tumor initiation, growth, and resistance to therapies. For the development of new CSC-targeted therapies, further in-depth studies are needed using enriched and stable MB-CSCs populations. This work, aimed at identifying the amount of CSCs in three available human cell lines (DAOY, D341, and D283), describes different approaches based on the expression of stemness markers. First, we explored potential differences in gene and protein expression patterns of specific stem cell markers. Then, in order to identify and discriminate undifferentiated from differentiated cells, MB cells were characterized using a physical characterization method based on a high-frequency dielectrophoresis approach. Finally, we compared their tumorigenic potential in vivo, through engrafting in nude mice. Concordantly, our findings identified the D283 human cell line as an ideal model of CSCs, providing important evidence on the use of a commercial human MB cell line for the development of new strategic CSC-targeting therapies.

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Section: Discussionsupporting

confidence: 84%

Human Medulloblastoma Cell Lines: Investigating on Cancer Stem Cell-Like Phenotype

Casciati

Tanori

Manczak

et al. 2020

Cancers

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“…In addition to revealing an underlying malignancy, the exosomal protein cargo functions to enhance the progression of metastasis of ovarian tumors ( Table 1). For example, Nanog is a transcription regulator involved in tumor cell proliferation and self-renewal of cancer stem cells [31]. Nanog expression is significantly greater in exosomes sampled from the ascites of high-grade serous ovarian cancer compared to benign peritoneal fluid [32].…”

Section: Ovarian Cancer-exosomesmentioning

confidence: 99%

Exosomes promote pre-metastatic niche formation in ovarian cancer

et al. 2019

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Ovarian cancer is one of the most common gynecological malignancies. Upon initial diagnosis, the majority of patients present with widespread metastatic growth within the peritoneal cavity. This metastatic growth occurs in stages, with the formation of a pre-metastatic niche occurring prior to macroscopic tumor cell invasion. Exosomes released by the primary ovarian tumor are small extracellular vesicles which prepare the distant tumor microenvironment for accelerated metastatic invasion. They regulate intercellular communication between tumor cells and normal stroma, cancer-associated fibroblasts, and local immune cells within the tumor microenvironment. In this review, we highlight the emerging roles of ovarian cancer exosomes as coordinators of pre-metastatic niche formation, biomarkers amenable to liquid biopsy, and targets of chemotherapy.Ovarian cancer is the deadliest gynecological malignancy, largely stemming from peritoneal metastasis. Formation of the pre-metastatic niche supports subsequent metastatic lesions. Ovarian cancer derived exosomes induce premetastatic niche formation via immunosuppression, angiogenesis, stromal cell remodeling, and oncogenic reprogramming. Ovarian cancer derived exosomes are promising biomarkers and therapeutic targets.

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“…Their role in normal adult tissues remains largely unknown and their activation is currently associated with cancer pathogenesis. Tumors positively genotyped for Oct4, Sox2, or Nanog are associated with a poor prognosis and increased metastasis by enabling cancerous cells to undergo sustained proliferation and evade cell death, suggesting that these genes may confer a stem-like state onto the cancer cells (Gong et al, 2015;Samardzija et al, 2012;Weina and Utikal, 2014).…”

Section: ) Osteogenesis Is Induced By Expression Of Runx2mentioning

confidence: 99%

Cartilage to bone transformation during fracture healing is coordinated by the invading vasculature and induction of the core pluripotency genes

et al. 2017

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Fractures heal predominantly through the process of endochondral ossification. The classic model of endochondral ossification holds that chondrocytes mature to hypertrophy, undergo apoptosis and new bone forms by invading osteoprogenitors. However, recent data demonstrate that chondrocytes transdifferentiate to osteoblasts in the growth plate and during regeneration, yet the mechanism(s) regulating this process remain unknown. Here, we show a spatiallydependent phenotypic overlap between hypertrophic chondrocytes and osteoblasts at the chondro-osseous border in the fracture callus, in a region we define as the transition zone (TZ). Hypertrophic chondrocytes in the TZ activate expression of the pluripotency factors [Sox2, Oct4 (Pou5f1), Nanog], and conditional knock-out of Sox2 during fracture healing results in reduction of the fracture callus and a delay in conversion of cartilage to bone. The signal(s) triggering expression of the pluripotency genes are unknown, but we demonstrate that endothelial cell conditioned medium upregulates these genes in ex vivo fracture cultures, supporting histological evidence that transdifferentiation occurs adjacent to the vasculature. Elucidating the cellular and molecular mechanisms underlying fracture repair is important for understanding why some fractures fail to heal and for developing novel therapeutic interventions.

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Regulation of NANOG in cancer cells

Cited by 87 publications

References 82 publications

Human Medulloblastoma Cell Lines: Investigating on Cancer Stem Cell-Like Phenotype

Human Medulloblastoma Cell Lines: Investigating on Cancer Stem Cell-Like Phenotype

Exosomes promote pre-metastatic niche formation in ovarian cancer

Cartilage to bone transformation during fracture healing is coordinated by the invading vasculature and induction of the core pluripotency genes

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