Regulation of Natriuretic Peptide Secretion by the Heart

Thibault, Gaétan; Amiri, Farhad; García, Raúl

doi:10.1146/annurev.physiol.61.1.193

Cited by 75 publications

(40 citation statements)

References 114 publications

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“…In previous studies with the teleost rainbow trout, endothelin-1 has been found to cause several short-term oscillations in blood pressure followed by a sustained hypertensive period (19,15,6). In mammals, it has been found that locally induced vasoactive agents, such as endothelin-1, mediate, in a paracrine fashion, part of the stretch-induced ANP secretion (29). Combined inhibition of endothelin and ANG II receptors has been reported to almost completely abolish the volume load-induced ANP release in vivo in rats (16).…”

Section: Discussionmentioning

confidence: 97%

Pronatriuretic peptide is a sensitive marker of the endocrine function of teleost heart

Vierimaa¹,

Hirvinen²,

Tervonen³

et al. 2002

American Journal of Physiology-Endocrinology and Metabolism

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We recently characterized a novel heart-specific hormone from salmon (salmon cardiac peptide, sCP). We have now prepared a recombinant plasmid expressing the NH2-terminal fragment of pro-sCP (NT-pro-sCP) and used it to set up a specific RIA for the peptide. Because of the sensitivity of the assay and the high circulating levels, NTpro-sCP can be measured from as little as 2 l of serum. This enables repeated sampling from the same animal in different experimental setups. Mechanical load increased the release of NT-pro-sCP from isolated perfused salmon ventricle, in parallel with sCP. Bolus injection of human endothelin-1 (ET-1; 1 g) in the dorsal aorta of salmon resulted in an extensive increase of serum NT-pro-sCP (from 0.99 Ϯ 0.11 to 4.6 Ϯ 1.5 nmol/l). The response was abolished by pretreatment with a specific type A ET (ETA) receptor antagonist (BQ-123) but not with a type B ET receptor antagonist (BQ-788).The NT-pro-sCP levels had a good correlation with those of sCP (r 2 ϭ 0.75). Our results demonstrate the practical usefulness of circulating NT-pro-sCP as a marker of the endocrine function of salmon heart. They also suggest that ET-1 has an important role in regulating sCP release from teleost heart by an ETA receptor-mediated mechanism. natriuretic peptide; endothelin; recombinant protein; radioimmunoassay; study in vivo NATRIURETIC PEPTIDES are cardiac hormones with an important role in regulating cardiovascular and fluid homeostasis (2). The release of A-and B-type natriuretic peptides (ANP and BNP) from the heart is stimulated by increased load (11,14,18) and by various paracrine factors, such as endothelin-1 (3, 16, 30). They reduce the cardiac load by causing natriuresis, diuresis, and relaxation of the vascular smooth muscle and by inhibiting the renin-angiotensin-aldosterone system (21). Although blood volume expansion is an important stimulus for ANP secretion in mammals, in nonmammalian eel, ANP secretion has been reported to be more sensitive to osmotic than volemic stimuli. In this teleost, ANP causes excretion of Na ϩ , thereby promoting adaptation to seawater (9).In mammals, ANP is stored in the secretory granules of atrial myocytes as a large-molecular-weight prohormone (proANP). It is processed to low-molecularweight ANP during exocytosis (33), presumably by the membrane-bound serine protease corin (36). The products are the 28-amino-acid biologically active ANP and the inert 98-amino-acid NH 2 -terminal fragment NTproANP (8,31). The elimination of ANP from the circulation is very rapid, resulting in low and labile plasma concentrations. The half-life of rat NT-proANP in rat circulation has been reported to be eight times longer than that of ANP (32). Therefore, and because its plasma concentrations are much higher, the measurement of NT-proANP is preferred over that of ANP in the assessment of cardiac function, e.g., in patients with congestive heart failure (22). The same appears to apply for .We have recently cloned and characterized from salmon a novel peptide hormone, salmon cardiac pep...

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Section: Discussionmentioning

confidence: 97%

Pronatriuretic peptide is a sensitive marker of the endocrine function of teleost heart

Vierimaa¹,

Hirvinen²,

Tervonen³

et al. 2002

American Journal of Physiology-Endocrinology and Metabolism

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“…The cardiac natriuretic peptides have been shown to be important for the regulation of the water and salt balance in both physiological and pathophysiological conditions in mammals; therefore, peptide hormones belonging to this family have been proposed to have an essential role in fish osmoregulation as well (9,10). In mammals, volemic stimuli are potent regulators of the cardiac natriuretic peptide release (25,36), whereas in the eel an increase in plasma osmolality rather than blood volume has been proposed to be a major regulator of eel atrial and ventricular natriuretic peptides (12). In the present study, we examined the effect of increased plasma volume and dehydration and enhanced osmolality on sCP in salmon.…”

Section: Discussionmentioning

confidence: 98%

“…In mammals, atrial stretch is the most widely recognized stimulus for ANP secretion (25,36). The increase of plasma volume, resulting in heart wall stretch, has been shown to elevate the circulating ANP concentration in a large number of animal species (1,12,14,26,37).…”

Section: Discussionmentioning

confidence: 99%

Salmon cardiac natriuretic peptide is a volume-regulating hormone

Tervonen¹,

Ruskoaho

Lecklin

et al. 2002

American Journal of Physiology-Endocrinology and Metabolism

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The present study tested the hypothesis that salmon cardiac peptide (sCP), a new member of the family of natriuretic peptides, has an important role in the regulation of fluid balance and cardiovascular function. Intra-arterial administration of sCP increased urine output in salmon. It had a diuretic effect in rat as well, but the potency was lower. sCP increased the sodium excretion in proportion to the increased urine flow. Blood pressure was not affected by sCP in either species. Acute volume expansion elevated the plasma level of sCP in salmon, and an acute transfer of salmon from fresh to sea water decreased the circulating sCP level. Cardiac immunoreactive sCP or sCP mRNA levels were not affected by transfer to sea water. These results indicate that sCP has an important physiological role in defending salmon against volume overload but that it does not appear to contribute to the short-term regulation of blood pressure. sCP provides an excellent model of the general mechanisms of regulation of the A-type (atrial) natriuretic peptide system.

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“…From the mechanistic point of view, these findings arise from pharmacological blockade of signaling implicating PLC in ANF release and hence, support the involvement of G␣ q and the previously reported tonic inhibition of ANF secretion by Ca 2ϩ . This ion, however, is required for normal basal as well as stimulated release by agonists such as ET-1 (50).…”

Section: Discussionmentioning

confidence: 99%

Phospholipase C signaling tonically represses basal atrial natriuretic factor secretion from the atria of the heart

Chang

McGrath

Bold

2013

American Journal of Physiology-Heart and Circulatory Physiology

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The cardiac hormone atrial natriuretic factor (ANF or ANP) plays significant, well-established roles in a large number of physiological and pathophysiological processes, including water and electrolyte balance, blood pressure regulation, and cardiovascular growth. Understanding the regulation of its production and secretion by atrial cardiomyocytes is incomplete. We have previously established a significant role of Gi/o protein signaling in modulating ANF secretion as promoted by stretch of the atrial myocardium. In the present study, we investigated the role of Gq protein signaling and its relationship to Gi/o protein signaling using pharmacological manipulation of proximal effectors of Gαq in an ex vivo model of spontaneously beating rat atria. Phospholipase C (PLC) and protein kinase C (PKC) inhibitors dramatically increased basal secretion of ANF. Furthermore, although atrial wall stretch is a potent stimulus for secretion, stretch unexpectedly reduced ANF secretion to basal levels under PLC and PKC inhibitory conditions. Inhibition of the inositol triphosphate receptor did not appear to affect basal secretion but dose-dependently blocked stretch-secretion coupling. The results obtained demonstrate that the PLC and PKC signaling cascades play important albeit unexpected roles in the regulation of basal and stimulated ANF secretion and suggest interplay between the Gq and Gi/o protein signaling pathways.

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Regulation of Natriuretic Peptide Secretion by the Heart

Cited by 75 publications

References 114 publications

Pronatriuretic peptide is a sensitive marker of the endocrine function of teleost heart

Pronatriuretic peptide is a sensitive marker of the endocrine function of teleost heart

Salmon cardiac natriuretic peptide is a volume-regulating hormone

Phospholipase C signaling tonically represses basal atrial natriuretic factor secretion from the atria of the heart

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