Regulation of nestin expression by thrombin and cell density in cultures of bone mesenchymal stem cells and radial glial cells

Wautier, F.; Wislet, Sabine; Chanas, Grazyna; Rogister, Bernard; Leprince, Pierre

doi:10.1186/1471-2202-8-104

Cited by 18 publications

(12 citation statements)

References 43 publications

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“…A similar phenomenon has already been described for glial precursor cells in which serum (through TGFβ factors) inhibited nestin expression [15]. Moreover, we previously demonstrated that thrombin was able to upregulate nestin expression by radial glial cells, but decreased the nestin expression in MSC cell population from bone marrow, indicating that the regulation of nestin expression could be finely modulated in various conditions and/or cell types [12].…”

Section: Discussionsupporting

confidence: 79%

“…We previously demonstrated that adult rat nestin-positive bone marrow stromal cells isolated from adult bone marrow were able to differentiate into excitable neurons, when co-cultured with mouse cerebellar granule neurons [11]. The proportion of nestin positive-cells in culture was quite low at passage 0, but would increase with reduced cell confluence [12] and higher passage numbers [10], until reaching a plateau (75% nestin- …”

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confidence: 99%

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RETRACTED ARTICLE: Mesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences

Wislet

Laudet

Neirinckx

et al. 2012

Cell. Mol. Life Sci.

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The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The recent identification in adult bone marrow of stem cells derived from the neural crest stem cells (NCSC) might explain the neuronal phenotypic plasticity shown by bone marrow cells. However, little information is available about the nature of these cells compared to mesenchymal stem cells (MSC), including their similarities and differences. In this paper, using transcriptomic as well as proteomic technologies, we compared NCSC to MSC and stromal nestin-positive cells, all of them isolated from adult bone marrow. We demonstrated that the nestin-positive cell population, which was the first to be described as able to differentiate into functional neurons, was a mixed population of NCSC and MSC. More interestingly, we demonstrated that MSC shared with NCSC the same ability to truly differentiate into Tuj1-positive cells when co-cultivated with paraformaldehyde-fixed cerebellar granule neurons. Altogether, those results suggest that both NCSC and MSC can be considered as important tools for cellular therapies in order to replace neurons in various neurological diseases. Running title:Characterization of neural crest and mesenchymal stem cells from adult bone marrow Author contribution:SWG : conception -design -collection of data -data analysis -manuscript writing EL : collection of data -data analysis -final approval of manuscript PA : collection of data VN : collection of data AG : collection of data CP : collection of data -data analysis -manuscript writing BH : collection of data -data analysis OS : Provision of study material LS : Provision of study material -final approval of manuscript PL: design-collection of data -data analysis -final approval of manuscript BR : Conception -design -Financial support -data analysis -final approval of manuscript Key wordsNeural crest stem cells; mesenchymal stem cells; adult bone marrow; cell fate; microarray ABSTRACT The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The recent identification in adult bone marrow of stem cells derived from the neural crest (NCSC) might explain the neuronal phenotypic plasticity shown by bone marrow cells. However, little information is available about the nature of these cells compared to mesenchymal stem cells (MSC), their similarities and differences. In this paper, using transcriptomic as well as proteomic technologies, we compared NCSC to MSC and stromal nestin-positive cells, all of them isolated from adult bone marrow. We demonstrated that the nestin-positive cell population, which was the first to be described as able to differentiate into functional neurons, was a mixed population of NCSC and MSC. More interestingly, we demonstrated that MSC shared with NCSC the same ability to t...

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Section: Discussionsupporting

confidence: 79%

mentioning

confidence: 99%

RETRACTED ARTICLE: Mesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences

Wislet

Laudet

Neirinckx

et al. 2012

Cell. Mol. Life Sci.

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“…Supplementation with FBS (MEM-20%) led to slight expression of a-SMA, whereas the immunoreactivity to nestin is lost. These results are in accordance with previous studies [16][17][18]. Furthermore, and most interestingly, we have demonstrated for the first time that stimulation of hMSC with GBM-conditioned medium results in concentrationdependent expression of desmin, CD 151, VE-cadherin, NG2, and nestin, and significantly increased expression of a-SMA.…”

Section: Gbm-conditioned Medium Induces Expression Of Pericyte Markersupporting

confidence: 93%

Glioblastoma-dependent differentiation and angiogenic potential of human mesenchymal stem cells in vitro

et al. 2011

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Tumor angiogenesis is of central importance in the malignancy of glioblastoma multiforme (GBM). As previously shown, human mesenchymal stem cells (hMSC) migrate towards GBM and are incorporated into tumor microvessels. However, phenotype and function of recruited hMSC remain unclear. We evaluated the differentiation and angiogenic potential of hMSC after stimulation with glioblastoma-conditioned medium in vitro. Immunostaining with endothelial, smooth muscle cell and pericyte markers was used to analyze hMSC differentiation in different concentrations of tumor-conditioned medium (CM), and the angiogenic potential was evaluated by matrigel-based tube-formation assay (TFA). Immunofluorescence staining revealed that tumor-conditioned hMSC (CM-hMSC) expressed CD 151, VE-cadherin, desmin, α-smooth muscle actin, nestin, and nerval/glial antigen 2 (NG2) in a CM concentration-dependent manner, whereas no expression of von-Willebrand factor (vWF) and smooth myosin could be detected. These findings are indicative of GBM-dependent differentiation of hMSC into pericyte-like cells, rather than endothelial or smooth muscle cells. Furthermore, TFA of hMSC and CM-hMSC revealed CM-dependent formation of capillary-like networks, which differed substantially from those formed by human endothelial cells (HUVEC), also implying pericyte-like tube formation. These results are indicative of GBM-derived differentiation of hMSC into pericyte-like mural cells, which might contribute to the neovascularization and stabilization of tumor vessels.

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“…In those conditions, we did observe an increase of the number of nestin-positive cells (presumable NCSC) in early passages. It has been previously shown that nestin expression was dependent on the number of passages [19,20], which allowed nestin-positive cell population to proliferate and take-over. In this study, wnt1 and BMP2 seem to accelerate NCSC proliferation and to favor or to recruit those cells over other cell types, until reaching a plateau after four passages.…”

Section: Discussionmentioning

confidence: 99%

Wnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow

Glejzer

Laudet

Leprince

et al. 2010

Cell. Mol. Life Sci.

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Recent studies have shown that neural crest-derived progenitor cells can be found in diverse mammalian tissues including tissues that were not previously shown to contain neural crest derivatives, such as bone marrow. The identification of those "new" neural crest-derived progenitor cells opens new strategies for developing autologous cell replacement therapies in regenerative medicine. However, their potential use is still a challenge as only few neural crest-derived progenitor cells were found in those new accessible locations. In this study, we developed a protocol, based on wnt1 and BMP2 effects, to enrich neural crest-derived cells from adult bone marrow. Those two factors are known to maintain and stimulate the proliferation of embryonic neural crest stem cells, however, their effects have never been characterized on neural crest cells isolated from adult tissues. Using multiple strategies from microarray to 2D-DIGE proteomic analyses, we characterized those recruited neural crest-derived cells, defining their identity and their differentiating abilities.

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Regulation of nestin expression by thrombin and cell density in cultures of bone mesenchymal stem cells and radial glial cells

Cited by 18 publications

References 43 publications

RETRACTED ARTICLE: Mesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences

RETRACTED ARTICLE: Mesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences

Glioblastoma-dependent differentiation and angiogenic potential of human mesenchymal stem cells in vitro

Wnt1 and BMP2: two factors recruiting multipotent neural crest progenitors isolated from adult bone marrow

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