Regulation of nicotine production in tobacco tissue culture by plant growth regulators

Tabata, Minoru; Yamamoto, Haruhiko; Hiraoka, Noboru; Marumoto, Yasumasa; Konoshima, Masao

doi:10.1016/s0031-9422(00)97139-5

Cited by 75 publications

(23 citation statements)

References 15 publications

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“…We think it could be of interest to perform further studies about the role of growth regulators in the excretion and uptake of secondary metabolites in order to learn more about the differences in the effect of 2,4-o and NAA. Similar to our results, NAA has shown to be favourable for the production of secondary metabolites as nicotine [28], shikonin derivatives [29], or anthraquinones [30]. In the case of Thalictrum minus cell cultures NAA showed a synergistic effect with BAP on the production of berberine [31].…”

Section: Discussionsupporting

confidence: 89%

Stability of alkaloid production in cell suspension cultures of Tabernaemontana divaricata during long-term subculture

Sierra

Heijden

Leer

et al. 1992

Plant Cell Tiss Organ Cult

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Two cell lines of Tabernaemontana divaricata cell suspension culture with different growth and alkaloid production profiles were transferred to the same medium. During 30 subcultures the changes in growth and alkaloid production were followed and compared to those of the original cell lines. The presence of NAA and BAP in the medium resulted in an increase of biomass and alkaloid yield. The effect on the growth proved to be stable during these 30 subcultures. Alkaloid production showed a maximum in the 4th subculture after the change of the medium, and stabilized on a higher level than found in the original cell lines. During some growth cycles also the activities of tryptophan decarboxylase (TDC), strictosidine synthase (SSS), and phenylalanineammonia-lyase (PAL) were measured. In both the original cell lines and the derived cell lines, growth and alkaloid production proved to be stable all through the experiment, although the derived cell lines had a period of adaptation to the new medium with increased productivity.

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Section: Discussionsupporting

confidence: 89%

Stability of alkaloid production in cell suspension cultures of Tabernaemontana divaricata during long-term subculture

Sierra

Heijden

Leer

et al. 1992

Plant Cell Tiss Organ Cult

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“…Pioneering work by Tabata et al [26] indicated that unorganized tissues of Scopololia parviflora cultured in vitro produced little or no alkaloids, but a close relation between primordia organization and alkaloid production in the cultures was observed [26]. In tobacco callus, there is a correlation between the absence of nicotine and the absence of bud organization [25]. More recently, some studies indicated the existence of a correlation between root organogenesis and alkaloid content in tobacco callus [I 5, 22], but others did not [17,27].…”

Section: Discussionmentioning

confidence: 99%

“…The relationship between organogenesis and production of alkaloids by tobacco callus cultures remains unclear. Some studies show the existence of a positive correlation between root organogenesis and nicotine levels [15,25], whereas others do not [17,27].…”

Section: Introductionmentioning

confidence: 99%

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Effect of polyamine biosynthetic inhibitors on alkaloids and organogenesis in tobacco callus cultures

Tiburcio

Kaur‐Sawhney

Galston

1987

Plant Cell Tiss Organ Cult

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We studied the effects of inhibitors of ornithine decarboxylase (ODC), arginine decarboxylase (ADC) and spermidine synthase (Spd synthase) on organogenesis and the titers of polyamines (PA) and alkaloids in tobacco calli. DL-alpha-diffluromethylarginine (DFMA) and D-arginine (D-Arg), both inhibitors of ADC activity, were more effective than DL-alpha-difluromethylorinithine (DFMO), an inhibitor of ODC, in reducing titers of PA and the putrescine (Put)-derived alkaloids (nornicotine and nicotine). Dicyclohexylammonium sulfate (DCHA), an inhibitor of Spd synthase, was also more efficient than DFMO in reducing PA and alkaloid levels. Root organogenesis is inversely related to the titers of Put and alkaloids. Thus, DFMA and D-Arg, which strongly inhibit Put and alkaloid biosynthesis, markedly promote root organogenesis, while control callus with high Put and alkaloid content showed poor root organization. These results suggest that morphological differentiation is not required for activation of secondary metabolic pathways and support the view that ADC has a major role in the generation of Put going to the pyrrolidine ring of tobacco alkaloids.

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“…glutamic acid. Tabata et al (1971) reported the accumulation of glutamic and aspartic acids in tobacco callus, when 2, 4-D was supplied at a low concentration (0.022 mg/l). These results suggest that the synthesis of glutamic acid in wheat callus is promoted by a considerably higher concentration of 2, 4-D than in tobacco callus.…”

Section: ) Esterase Isozymes In Wheat Callusmentioning

confidence: 99%

<i>IN VITRO</i> CULTURE OF WHEAT TISSUES. II. MORPHOLOGICAL, CYTOLOGICAL AND ENZYMATIC VARIATIONS INDUCED IN WHEAT CALLUS BY GROWTH REGULATORS, ADENINE SALFATE AND CASEIN HYDROLYSATE

Nakai

Shimada

1975

The Japanese journal of genetics

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Regulation of nicotine production in tobacco tissue culture by plant growth regulators

Cited by 75 publications

References 15 publications

Stability of alkaloid production in cell suspension cultures of Tabernaemontana divaricata during long-term subculture

Stability of alkaloid production in cell suspension cultures of Tabernaemontana divaricata during long-term subculture

Effect of polyamine biosynthetic inhibitors on alkaloids and organogenesis in tobacco callus cultures

<i>IN VITRO</i> CULTURE OF WHEAT TISSUES. II. MORPHOLOGICAL, CYTOLOGICAL AND ENZYMATIC VARIATIONS INDUCED IN WHEAT CALLUS BY GROWTH REGULATORS, ADENINE SALFATE AND CASEIN HYDROLYSATE

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