Regulation of Nitric Oxide Production by Arginine Metabolic Enzymes

Cymbopogon citratus as source of new and safe anti-inflammatory drugs: Bio-guided assay using lipopolysaccharide-stimulated macrophages

“…All the fractions inhibited iNOS expression while only PAF and TF inhibited NO production, suggesting that polyphenolic fractions modulate not only the iNOS expression but also its activity. Accordingly, recent studies demonstrate that polyphenols could modify the iNOS activity by modulating the availability of L-arginine, the ratelimiting substrate of iNOS (Mori and Gotoh, 2000). We also previously demonstrated that Cy extract and its polyphenols have iNOS and NO inhibitory properties in dendritic cells (Figueirinha et al, 2010).…”

Modulation of some markers of erectile dysfunction and malonaldehyde levels in isolated rat penile tissue with unripe and ripe plantain peels: identification of the constituents of the plants using HPLC

“…The PDE-5 inhibitory potentials of the studied peels could be linked to their rich flavonoid. The utilization of L-arginine by arginase activity in vascular endothelia and smooth muscle cells of penile tissue can reduce NOS activity and consequently decrease concentration of NO: a major factor in erectile function (Mori and Gotoh 2000). Hence, the inhibition of arginase activity can be additional therapeutic targets for the management of ED as this could increase the bioavailability of L-arginine, contribute to the production of NO via reaction catalyzed by NOS, where overall result could facilitate penile erection.…”

Immunomodulatory effects of recombinant BCG expressing MSP-1C ofPlasmodium falciparumon LPS- or LPS+IFN-γ-stimulated J774A.1 cells

“…As expected, a strong iNOS protein expression was observed in rBCG-infected macrophages compared with BCGinfected macrophages or uninfected macrophages, suggesting that iNOS enzyme was involved in regulating NO production by the macrophages. 63,64 NO and iNOS productions have been reported to be correlated with TNF-α and IL-1β productions since both processes involved NF-κB and MAPKinases regulation. 65,66 In this study, both BCG and rBCG infections also stimulated higher production of TNF-α and IL-1β, suggesting the involvement of these cytokines in stimulating the production of NO and iNOS by the macrophages.…”