Regulation of nitric oxide synthesis in the liver

Muriel, Pablo

doi:10.1002/(sici)1099-1263(200005/06)20:3<189::aid-jat632>3.0.co;2-8

Cited by 59 publications

(26 citation statements)

References 71 publications

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“…In addition, studies in cholestatic rats have shown an association with hepatic ischemia because of the deficient production of iNOS and protein. This supports the hypothesis that NO regulates blood flow in cholestatic rats [17, 18]. …”

Section: Discussionsupporting

confidence: 89%

The Effects of L-Arginine on Liver Damage in Experimental Acute Cholestasis an Immunohistochemical Study

et al. 2011

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Obstructive jaundice damages critical functions in the liver. Nitric oxide modulation would influence liver damage induced by biliary obstruction, and little is known about it Acute cholestasis was induced by bile duct ligation (BDL) in two groups of male Sprague-Dawley rats. L-Arginine or serum physiologic was administered to treatment and control group. Histopathological and immunohistochemical iNOS expression was investigated in hepatic tissue. Plasma enzyme activities were increased in acute cholestasis, and that L-arginine treatment partially but significantly prevented the elevation of these markers of liver damage (P < .05). Also histopathology scoring showed that the liver injury was prevented and immunohistochemical iNOS activity was increased significantly in L-arginine group (P < .05). This study shows that, after 7 days of biliary obstruction, liver damage is well established and exogenous L-arginine treatment partially but significantly prevented the liver injury in acute cholestasis.

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Section: Discussionsupporting

confidence: 89%

The Effects of L-Arginine on Liver Damage in Experimental Acute Cholestasis an Immunohistochemical Study

et al. 2011

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“…Citrulline is produced both during the urea cycle and from the oxidation of arginine by nitric oxide synthases (Muriel 2000). Citrulline is a marker of intestinal absorption and function, with increased levels correlating with increased absorptive function (Jianfeng et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Consumption of pasteurized human lysozyme transgenic goats’ milk alters serum metabolite profile in young pigs

et al. 2009

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Nutrition, bacterial composition of the gastrointestinal tract, and general health status can all influence the metabolic profile of an organism. We previously demonstrated that feeding pasteurized transgenic goats’ milk expressing human lysozyme (hLZ) can positively impact intestinal morphology and modulate intestinal microbiota composition in young pigs. The objective of this study was to further examine the effect of consuming hLZ-containing milk on young pigs by profiling serum metabolites. Pigs were placed into two groups and fed a diet of solid food and either control (non-transgenic) goats’ milk or milk from hLZ-transgenic goats for 6 weeks. Serum samples were collected at the end of the feeding period and global metabolite profiling was performed. For a total of 225 metabolites (160 known, 65 unknown) semi-quantitative data was obtained. Levels of 18 known and 4 unknown metabolites differed significantly between the two groups with the direction of change in 13 of the 18 known metabolites being almost entirely congruent with improved health status, particularly in terms of the gastrointestinal tract health and immune response, with the effects of the other five being neutral or unknown. These results further support our hypothesis that consumption of hLZ-containing milk is beneficial to health.

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“…In the liver, LPS triggers the production of reactive oxygen species, including superoxide [32]. LPS also stimulates iNOS activity In the liver and vascular cells, which leads to increased production of nitric-oxide [33]. In addition to systemic hypotension and poor perfusion, large amounts of nitric-oxide produce direct hepatotoxic effects [34].…”

Section: Resultsmentioning

confidence: 99%

Cationic peptide mR18L with lipid lowering properties inhibits LPS-induced systemic and liver inflammation in rats

Sharifov

Nayyar

Ternovoy

et al. 2013

Biochemical and Biophysical Research Communications

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The cationic single domain peptide mR18L has demonstrated lipid-lowering and anti-atherogenic properties in different dyslipidemic mouse models. Lipopolysaccharide (LPS)-mediated inflammation is considered as one of the potential triggers for atherosclerosis. Here, we evaluated anti-inflammatory effects of mR18L peptide against LPS-mediated inflammation. First, we tested the efficacy and tolerance of 1, 2.5 and 5 mg/kg mR18L in normolipidemic rats stimulated with 5 mg/kg LPS. LPS and then mR18L were injected in different intraperitoneal regions. By 2 hours post LPS, mR18L inhibited LPS-mediated plasma TNF-α elevation at all doses, with the effect being stronger for 2.5 mg/kg (P<0.05 vs. 1 mg/kg, non-significant vs. 5 mg/kg). In a similar model, 2.5 mg/kg mR18L reduced LPS-mediated inflammation in the liver, as assessed by microscopic examination of liver sections and measurements of iNOS expression in the liver tissue. In plasma, 2.5 mg/kg mR18L decreased levels of TNF-α and IL-6, decreased endotoxin activity and enhanced HDL binding to LPS. In another similar experiment, mR18L administered 1h post LPS, prevented elevation of plasma triglycerides by 6h post LPS and increased plasma activity of anti-oxidant enzyme paraoxonase 1, along with noted trends in reducing plasma levels of endotoxin and IL-6. Surface plasmon resonance study revealed that mR18L readily binds LPS. We conclude that mR18L exerts anti-endotoxin activity at least in part due to direct LPS-binding and LPS-neutralizing effects. We suggest that anti-endotoxin activity of mR18L is an important anti-inflammatory property, which may increase anti-atherogenic potential of this promising orally active lipid-lowering peptide.

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Regulation of nitric oxide synthesis in the liver

Cited by 59 publications

References 71 publications

The Effects of L-Arginine on Liver Damage in Experimental Acute Cholestasis an Immunohistochemical Study

The Effects of L-Arginine on Liver Damage in Experimental Acute Cholestasis an Immunohistochemical Study

Consumption of pasteurized human lysozyme transgenic goats’ milk alters serum metabolite profile in young pigs

Cationic peptide mR18L with lipid lowering properties inhibits LPS-induced systemic and liver inflammation in rats

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