Regulation of Nitrogen Fixation in Herbaspirillum seropedicae

Pedrosa, F. O.; Souza, Emanuel Maltempi de; Benelli, Elaine Machado; Machado, Iara; Steffens, Maria; Klassen, Giseli; Machado, Hidevaldo B.; Rigo, L. U.; Wassem, Roseli; Monteiro, Rose Adele; Rego, F. G. M.; Chubats, L. S.; Buck, Martin; Moore, M.; Harper, Andrea L.; Drummond, Martin; Yates, M. G.

doi:10.1007/978-94-011-5159-7_43

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…In diazotrophic members of the K-group of Proteobacteria, to which Acetobacter diazotrophicus belongs, and in Herbaspirillum seropedicae, a L-group member, inactivation of NifA by oxygen occurs by inherent sensitivity of NifA protein to oxygen damage apparently via a Cys-aa 4 -Cys motif in the interdomain linker region [10]. Mechanisms for inactivation of NifA in ammonium-grown cells of some diazotrophs of the K-group have not been well-characterized, but in the case of A. brasilense and H. seropedicae, this occurs through the N-terminal domain of NifA, the most variable of the regions of NifA proteins [11,12]. The other NifA domains include a highly conserved central region involved in ATP hydrolysis essential for conversion of closed to open complex of RNA polymerase bound to the promoter region of NifA-activated nif/¢x genes, an interdomain linker, involved in the oxygen-dependent regulation of NifA activity [10], and a C-terminal domain, essential for the speci¢c NifA interaction with susceptible promoter sequences (reviewed in [2]).…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of the chromosomal region encoding the nifA and nifB genes of Acetobacter diazotrophicus

Teixeira¹

1999

FEMS Microbiology Letters

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The Acetobacter diazotrophicus nifA gene was isolated by its ability to restore a Nif phenotype to a nifA mutant of Azotobacter vinelandii. Sequencing revealed that the nifA gene was upstream and adjacent to the nifB gene and both are transcribed in the same direction but independently from different promoters. The 3P end of the nifB gene was located approximately 2.5 kb upstream of the nitrogenase structural gene cluster, nifHDK. The deduced amino acid sequences of the A. diazotrophicus nifA and nifB gene products were most similar to the NifA and NifB proteins of Azorhizobium caulinodans and Rhodobacter capsulatus, respectively. A. diazotrophicus nifA expression was repressed in cultures exposed to high levels of ammonium while oxygen apparently had no influence. Both oxygen and ammonium prevented expression of a nifB-reporter strain, consistent with the observation that ammonium repressed nifA expression, and indicating that A. diazotrophicus NifA activity is inhibited by oxygen as in other Proteobacterial K group diazotrophs. ß

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Section: Introductionmentioning

confidence: 99%

Molecular analysis of the chromosomal region encoding the nifA and nifB genes of Acetobacter diazotrophicus

Teixeira¹

1999

FEMS Microbiology Letters

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Recent developments in the structural organization and regulation of nitrogen fixation genes in Herbaspirillum seropedicae

Pedrosa

Benelli

Yates

et al. 2001

Journal of Biotechnology

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Molecular analysis of the chromosomal region encoding thenifAandnifBgenes ofAcetobacter diazotrophicus

Teixeira

Wülling

Morgan

et al. 1999

FEMS Microbiology Letters

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The Acetobacter diazotrophicus nifA gene was isolated by its ability to restore a Nif+ phenotype to a nifA mutant of Azotobacter vinelandii. Sequencing revealed that the nifA gene was upstream and adjacent to the nifB gene and both are transcribed in the same direction but independently from different promoters. The 3′ end of the nifB gene was located approximately 2.5 kb upstream of the nitrogenase structural gene cluster, nifHDK. The deduced amino acid sequences of the A. diazotrophicus nifA and nifB gene products were most similar to the NifA and NifB proteins of Azorhizobium caulinodans and Rhodobacter capsulatus, respectively. A. diazotrophicus nifA expression was repressed in cultures exposed to high levels of ammonium while oxygen apparently had no influence. Both oxygen and ammonium prevented expression of a nifB‐reporter strain, consistent with the observation that ammonium repressed nifA expression, and indicating that A. diazotrophicus NifA activity is inhibited by oxygen as in other Proteobacterial α group diazotrophs.

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Regulation of Nitrogen Fixation in Herbaspirillum seropedicae

Cited by 3 publications

References 8 publications

Molecular analysis of the chromosomal region encoding the nifA and nifB genes of Acetobacter diazotrophicus

Molecular analysis of the chromosomal region encoding the nifA and nifB genes of Acetobacter diazotrophicus

Recent developments in the structural organization and regulation of nitrogen fixation genes in Herbaspirillum seropedicae

Molecular analysis of the chromosomal region encoding thenifAandnifBgenes ofAcetobacter diazotrophicus

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