Regulation of NUCB2/nesfatin-1 production in rat's stomach and adipose tissue is dependent on age, testosterone levels and lactating status

Senín, Lucía L.; Al‐Massadi, Omar; Barja-Fernández, Silvia; Folgueira, Cintia; Castelao, Cecilia; Tovar, Sulay; Leis, Rosaura; Lago, Francisca; Baltar, Javier; Baamonde, Iván; Diéguez, Carlos; Casanueva, Felipe F.; Seoane, Luísa M.

doi:10.1016/j.mce.2015.04.016

Cited by 22 publications

(13 citation statements)

References 36 publications

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“…As previously reported 1 3 24 25 26 , the gastrointestinal tract is crucial for energy homeostasis regulation. Currently, an increasing number of signals secreted from the gastrointestinal tract are proposed as components to the gastrointestinal-brain-adipose tissue axe in charge of body weight regulation 24 25 26 . FNDC5/irisin was initially described in muscle 10 and later in adipose tissue 12 .…”

Section: Discussionmentioning

confidence: 55%

FNDC5 is produced in the stomach and associated to body composition

Barja-Fernández

Folgueira

Castelao

et al. 2016

Sci Rep

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The fibronectin type III domain-containing protein 5 (FNDC5) discovered in 2002 has recently gained attention due to its potential role in protecting against obesity. In rat, no data exist regarding FNDC5 production and regulation in the stomach. The aim of the present work was to determine the expression of FNDC5 in the rat stomach and its potential regulation by body composition. The present data shows FNDC5 gene expression in the gastric mucosa. Immunohistochemical studies found FNDC5 immunopositivity in chief cells of gastric tissue. By the use of three different antibodies FNDC5 was found expressed in gastric mucosa and secreted by the stomach. The rate of gastric FNDC5 secretion parallels the circulating levels of FNDC5. The body fat mass increase after intervention with high fat diet coincided with a decrease in the secretion of FNDC5 from the stomach and a diminution in the FNDC5 circulating levels. In summary, the present data shows, for the first time, the expression of FNDC5 in the stomach of rats and its regulation by body composition, suggesting a potential role of gastric FNDC5 in energy homeostasis.

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Section: Discussionmentioning

confidence: 55%

FNDC5 is produced in the stomach and associated to body composition

Barja-Fernández

Folgueira

Castelao

et al. 2016

Sci Rep

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“…Indeed, several reports have clarified the role of expression of nesfatin-1 in the stomach and subcutaneous adipose tissue in controlling energy balance throughout development. The results indicated that stomach and the adipose tissue acted on the regulation of circulating nesfatin-1 levels to adapt to different requirements with development1617. We found nesfatin-1 IR in cells and altered expression of NUCB2 mRNA within the HPGA with age, suggesting that nesfatin-1 plays a role in regulating reproduction by modulating the HPGA in male rats.…”

Section: Discussionmentioning

confidence: 65%

Role of Nesfatin-1 in the Reproductive Axis of Male Rat

Gao

Zhang

Song

et al. 2016

Sci Rep

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Nesfatin-1 is an important molecule in the regulation of reproduction. However, its role in the reproductive axis in male animals remains to be understood. Here, we found that nesfatin-1 was mainly distributed in the arcuate nucleus (ARC), paraventricular nucleus (PVN), periventricular nucleus (PeN), and lateral hypothalamic area (LHA) of the hypothalamus; adenohypophysis and Leydig cells in male rats. Moreover, the concentrations of serum nesfatin-1 and its mRNA in hypothalamo-pituitary-gonadal axis (HPGA) vary with the age of the male rat. After intracerebroventricular injection of nesfatin-1, the hypothalamic genes for gonadotrophin releasing hormone (GnRH), kisspeptin (Kiss-1), pituitary genes for follicle-stimulate hormone β(FSHβ), luteinizing hormone β(LHβ), and genes for testicular steroidogenic acute regulatory (StAR) expression levels were decreased significantly. Nesfatin-1 significantly increased the expression of genes for 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid dehydrogenase (17β-HSD), and cytochrome P450 cleavage (P450scc) in the testis of pubertal rats, but their levels decreased in adult rats (P < 0.05), along with the serum FSH, LH, and testosterone (T) concentrations. After nesfatin-1 addition in vitro, T concentrations of the supernatant were significantly higher than that in the control group. These results were suggestive of the role of nesfatin-1 in the regulation of the reproductive axis in male rats.

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“…Mohan and Unniappan [22] demonstrated that NUCB2/Nesf-1 immunoreactivity in the pancreas and gastrointestinal tract of rats increased from embryonic day 21 through to postnatal day 27, which is likely related to weaning. Senin and colleagues [23] confirmed and expanded previous data on gastroenteropancreatic tissues of rats by analyzing NUCB2/Nesf-1 expression from 2 until 8 weeks of age, corresponding to adulthood. However, there are no reports regarding age-related changes of NUCB2/Nesf-1 in the brain and stomach of any vertebrate.…”

Section: Introductionmentioning

confidence: 53%

Ontogenetic Pattern Changes of Nucleobindin-2/Nesfatin-1 in the Brain and Intestinal Bulb of the Short Lived African Turquoise Killifish

Montesano

Felice

Leggieri

et al. 2019

JCM

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Nesfatin-1 (Nesf-1) was identified as an anorexigenic and well conserved molecule in rodents and fish. While tissue distribution of NUCB2 (Nucleobindin 2)/Nesf-1 is discretely known in vertebrates, reports on ontogenetic expression are scarce. Here, we examine the age-related central and peripheral expression of NUCB2/Nesf-1 in the teleost African turquoise killifish Nothobranchius furzeri, a consolidated model organism for aging research. We focused our analysis on brain areas responsible for the regulation of food intake and the rostral intestinal bulb, which is analogous of the mammalian stomach. We hypothesize that in our model, the stomach equivalent structure is the main source of NUCB2 mRNA, displaying higher expression levels than those observed in the brain, mainly during aging. Remarkably, its expression significantly increased in the rostral intestinal bulb compared to the brain, which is likely due to the typical anorexia of aging. When analyzing the pattern of expression, we confirmed the distribution in diencephalic areas involved in food intake regulation at all age stages. Interestingly, in the rostral bulb, NUCB2 mRNA was localized in the lining epithelium of young and old animals, while Nesf-1 immunoreactive cells were distributed in the submucosae. Taken together, our results represent a useful basis for gaining deeper knowledge regarding the mechanisms that regulate food intake during vertebrate aging.

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Regulation of NUCB2/nesfatin-1 production in rat's stomach and adipose tissue is dependent on age, testosterone levels and lactating status

Cited by 22 publications

References 36 publications

FNDC5 is produced in the stomach and associated to body composition

FNDC5 is produced in the stomach and associated to body composition

Role of Nesfatin-1 in the Reproductive Axis of Male Rat

Ontogenetic Pattern Changes of Nucleobindin-2/Nesfatin-1 in the Brain and Intestinal Bulb of the Short Lived African Turquoise Killifish

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