Regulation of OCT4 gene expression by liver receptor homolog-1 in human embryonic carcinoma cells

Sung, BoReum; Do, Hyun-Jin; Park, Sung-Won; Huh, Sun-Hyung; Oh, Jonghyun; Chung, Hak-Jae; Kang, Man‐Jong; Kim, Jin‐Hoi; Kim, Nam‐Hyung; Kim, Jaehwan

doi:10.1016/j.bbrc.2012.09.049

Cited by 15 publications

(21 citation statements)

References 38 publications

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“…Previously, we showed that OCT4 expression is repressed during RA-mediated differentiation in NCCIT cells. 24,25) Indeed, OCT4 expression was significantly downregulated in RA-treated NCCIT cells, confirming that the cells differentiated (Fig. 1B).…”

Section: Resultssupporting

confidence: 60%

Differential Expression of ETS Family Transcription Factors in NCCIT Human Embryonic Carcinoma Cells upon Retinoic Acid-Induced Differentiation

Park

et al. 2014

Biological & Pharmaceutical Bulletin

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E26 transformation-specific (ETS) transcription factors play important roles in normal and tumorigenic processes during development, differentiation, homeostasis, proliferation, and apoptosis. To identify critical ETS factor(s) in germ cell-derived cancer cells, we examined the expression patterns of the 27 ETS transcription factors in naive and differentiated NCCIT human embryonic carcinoma cells, which exhibit both pluripotent and tumorigenic characteristics. Overall, expression of ETS factors was relatively low in NCCIT cells. Among the 27 ETS factors, polyomavirus enhancer activator 3 (PEA3) and epithelium-specific ETS transcription factor-1 (ESE-1) exhibited the most significant changes in their expression levels. Western blot analysis confirmed these patterns, revealing reduced levels of PEA3 protein and elevated levels of ESE-1 protein in differentiated cells. PEA3 increased the proportion of cells in S-phase and promoted cell growth, whereas ESE-1 reduced proliferation potential. These data suggest that PEA3 and ESE-1 may play important roles in pluripotent and tumorigenic embryonic carcinoma cells. These findings contribute to our understanding of the functions of oncogenic ETS factors in germ cell-derived stem cells during processes related to tumorigenesis and pluripotency.

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Section: Resultssupporting

confidence: 60%

Differential Expression of ETS Family Transcription Factors in NCCIT Human Embryonic Carcinoma Cells upon Retinoic Acid-Induced Differentiation

Park

et al. 2014

Biological & Pharmaceutical Bulletin

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“…38) We also previously reported that OCT4 and GDF3 are highly expressed in pluripotent human EC NCCIT cells and that this expression is reduced upon RA treatment in a time-dependent manner. 30,36,37,39,40) However, there is no report concerning the interaction between OCT4 and GDF3 at the transcriptional level. Therefore, to investigate their transcriptional relationship in pluripotent germ cell-derived EC cells, we first examined the expression of GDF3 and OCT4 in NCCIT cells during RA-mediated differentiation.…”

Section: Resultsmentioning

confidence: 99%

Growth and Differentiation Factor 3 Is Transcriptionally Regulated by OCT4 in Human Embryonic Carcinoma Cells

Han

Park

et al. 2016

Biological & Pharmaceutical Bulletin

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Growth and differentiation factor 3 (GDF3), a mammalian-specific transforming growth factor β ligand, and OCT4, one of key stem cell transcription factors, are expressed in testicular germ cell tumors (TGCTs) as well as pluripotent stem cells. To understand the molecular mechanism by which OCT4 and GDF3 function in tumorigenesis as well as stemness, we investigated the transcriptional regulation of GDF3 mediated by OCT4 in human embryonic carcinoma (EC) NCCIT cells, which are pluripotent stem cells of TGCTs. GDF3 and OCT4 was highly expressed in undifferentiated NCCIT cells and then significantly decreased upon retinoic acid-induced differentiation in a time-dependent manner. Moreover, GDF3 expression was reduced by short hairpin RNA-mediated knockdown of OCT4 and increased by OCT4 overexpression, suggesting that GDF3 and OCT4 have a functional relationship in pluripotent stem cells. A promoter-reporter assay revealed that the GDF3 promoter ( 1721-Luc) activity was significantly activated by OCT4 in a dose-dependent manner. Moreover, the minimal promoter ( 183-Luc) was sufficient for OCT4-mediated transcriptional activation and provided a potential binding site for the direct interaction with OCT4. Collectively, this study provides the evidence about the regulatory mechanism of GDF3 mediated by OCT4 in pluripotent EC cells.

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“…Expression is lost during differentiation [219][220][221][222][223]. Reciprocally, OCT3/4 downregulation initiates differentiation [216] or, in PGCs, apoptosis [217,224,225].…”

Section: Oct3/4 and Nanogmentioning

confidence: 99%

An oncofetal and developmental perspective on testicular germ cell cancer

Rijlaarsdam

Looijenga

2014

Seminars in Cancer Biology

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Regulation of OCT4 gene expression by liver receptor homolog-1 in human embryonic carcinoma cells

Cited by 15 publications

References 38 publications

Differential Expression of ETS Family Transcription Factors in NCCIT Human Embryonic Carcinoma Cells upon Retinoic Acid-Induced Differentiation

Differential Expression of ETS Family Transcription Factors in NCCIT Human Embryonic Carcinoma Cells upon Retinoic Acid-Induced Differentiation

Growth and Differentiation Factor 3 Is Transcriptionally Regulated by OCT4 in Human Embryonic Carcinoma Cells

An oncofetal and developmental perspective on testicular germ cell cancer

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