Regulation of Osmotic Balance and Increased Antioxidant Activities under Heat Stress in Abelmoschus esculentus L. Triggered by Exogenous Proline Application

Hussain, Rashid; Ayyub, C. M.; Shaheen, Muhammad Rashid; Rashid, Sohail; Nafees, Muhammad; Ali, Syed Shahid; Butt, Madiha; Ali, Muhammad; Maqsood, Ambreen; Fiaz, Sajid; Ahmar, Sunny; Mahmood, Tahir; Mora‐Poblete, Freddy

doi:10.3390/agronomy11040685

Cited by 26 publications

(7 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CAT and POD convert the toxic H 2 O 2 into water and oxygen ( Farooq et al, 2018 ). An increase in CAT, TSP, and POD contents is generally observed in high-yielding cultivars as these are actively involved in scavenging H 2 O 2 to maintain its optimum level ( Gosavi et al, 2014 ; Hussain et al, 2021 ). The genotypes that showed higher CAT, POD, and TSP values were optimal for H 2 O 2 level and were declared as heat-tolerant genotypes.…”

Section: Discussionmentioning

confidence: 99%

Unraveling Heat Tolerance in Upland Cotton (Gossypium hirsutum L.) Using Univariate and Multivariate Analysis

et al. 2022

View full text Add to dashboard Cite

The ever-changing global environment currently includes an increasing ambient temperature that can be a devastating stress for organisms. Plants, being sessile, are adversely affected by heat stress in their physiology, development, growth, and ultimately yield. Since little is known about the response of biochemical traits to high-temperature ambiance, we evaluated eight parental lines (five lines and three testers) and their 15 F1 hybrids under normal and high-temperature stress to assess the impact of these conditions over 2 consecutive years. The research was performed under a triplicate randomized complete block design including a split-plot arrangement. Data were recorded for agronomic, biochemical, and fiber quality traits. Mean values of agronomic traits were significantly reduced under heat stress conditions, while hydrogen peroxide, peroxidase, total soluble protein, superoxide dismutase, catalase (CAT), carotenoids, and fiber strength displayed higher mean values under heat stress conditions. Under both conditions, high genetic advance and high heritability were observed for seed cotton yield (SCY), CAT, micronaire value, plant height, and chlorophyll-a and b content, indicating that an additive type of gene action controls these traits under both the conditions. For more insights into variation, Pearson correlation analysis and principal component analysis (PCA) were performed. Significant positive associations were observed among agronomic, biochemical, and fiber quality-related traits. The multivariate analyses involving hierarchical clustering and PCA classified the 23 experimental genotypes into four groups under normal and high-temperature stress conditions. Under both conditions, the F1 hybrid genotype FB-SHAHEEN × JSQ WHITE GOLD followed by Ghuari-1, CCRI-24, Eagle-2 × FB-Falcon, Ghuari-1 × JSQ White Gold, and Eagle-2 exhibited better performance in response to high-temperature stress regarding the agronomic and fiber quality-related traits. The mentioned genotypes could be utilized in future cotton breeding programs to enhance heat tolerance and improve cotton yield and productivity through resistance to environmental stressors.

show abstract

Section: Discussionmentioning

confidence: 99%

Unraveling Heat Tolerance in Upland Cotton (Gossypium hirsutum L.) Using Univariate and Multivariate Analysis

et al. 2022

View full text Add to dashboard Cite

show abstract

“…As a multi-functional molecule, Pro accumulated during a stress episode is degraded to provide a supply of energy to drive growth once the stress is relieved (Hayat et al, 2012 ; Kishor and Sreenivasulu, 2014 ). Furthermore, exogenous Pro plays an important role in improving and enhancing plant stress resistance (Hayat et al, 2012 ; Hussain et al, 2021 ). Hence, the Pro concentration increased in the HN treatment and decreased as melatonin alleviates high-nitrate stress.…”

Section: Discussionmentioning

confidence: 99%

Effects of Melatonin on Morphological Characteristics, Mineral Nutrition, Nitrogen Metabolism, and Energy Status in Alfalfa Under High-Nitrate Stress

2021

View full text Add to dashboard Cite

Melatonin is an indoleamine small molecular substance that has been shown to play an important role in the growth, development, and stress response of plants. The effects of melatonin on the morphological characteristics, mineral nutrition, nitrogen metabolism, and energy status in alfalfa (Medicago sativa L.) under high-nitrate stress were studied. The alfalfa plants were treated with water (CK), 200 mmol L−1 nitrates (HN), or 200 mmol L−1 nitrates + 0.1 mmol L−1 melatonin (HN+MT), and then were sampled for measurements on days 0 and 10, respectively. The results showed that the HN treatment resulted in a decrease in the morphological characteristics (such as shoot height, leaf length, leaf width, leaf area, and biomass), phosphorus, soluble protein (SP), nitrogen-related enzymes activities and gene relative expression, adenosine triphosphate (ATP), and energy charge (EC). It also caused an increase in nitrogen, sodium, potassium, calcium, nitrate-nitrogen (NO3--N), ammonium-nitrogen (NH4+-N), adenosine diphosphate (ADP), and adenosine monophosphate (AMP). However, these parameters were conversely changed in the HN+MT treatment. Besides, these parameters were closely related to each other, and were divided into two principal components. It reveals that melatonin plays an important role in modulating the morphology, mineral nutrition, nitrogen metabolism and energy status, thereby alleviating the adverse effects of high-nitrate stress and improving the growth of alfalfa.

show abstract

“…Recently, Hussain et al . (2021) subjected 4‐week‐old okra ( Abelmoschus esculentus L.) seedlings to HS extending from 28/22 to 45/35 °C (day/night), where foliar spray application of 2.5 m M proline resulted in an optimum increase in root and shoot biomass, leaves per plant, and water use efficiency under HS, accompanied by an increase in activity of antioxidant enzymes. Similarly, sugar beet ( Beta vulgaris L.) under HS (50 and 55 °C) given foliar proline (25, 50, 100 mg·L −1 ) had improved height, root morphology, and biomass through increases in photosynthetic pigments, soluble sugars, and amino acids (Khalil et al .…”

Section: Exogenous Application Of Proline Mitigates Extreme Temperatu...mentioning

confidence: 99%

Assessment of proline function in higher plants under extreme temperatures

et al. 2023

View full text Add to dashboard Cite

Climate change and abiotic stress factors are key players in crop losses worldwide. Among which, extreme temperatures (heat and cold) disturb plant growth and development, reduce productivity and, in severe cases, lead to plant death. Plants have developed numerous strategies to mitigate the detrimental impact of temperature stress. Exposure to stress leads to the accumulation of various metabolites, e.g. sugars, sugar alcohols, organic acids and amino acids. Plants accumulate the amino acid 'proline' in response to several abiotic stresses, including temperature stress. Proline abundance may result from de novo synthesis, hydrolysis of proteins, reduced utilization or degradation. Proline also leads to stress tolerance by maintaining the osmotic balance (still controversial), cell turgidity and indirectly modulating metabolism of reactive oxygen species. Furthermore, the crosstalk of proline with other osmoprotectants and signalling molecules, e.g. glycine betaine, abscisic acid, nitric oxide, hydrogen sulfide, soluble sugars, helps to strengthen protective mechanisms in stressful environments. Development of less temperature-responsive cultivars can be achieved by manipulating the biosynthesis of proline through genetic engineering. This review presents an overview of plant responses to extreme temperatures and an outline of proline metabolism under such temperatures. The exogenous application of proline as a protective molecule under extreme temperatures is also presented. Proline crosstalk and interaction with other molecules is also discussed. Finally, the potential of genetic engineering of proline-related genes is explained to develop 'temperature-smart' plants. In short, exogenous application of proline and genetic engineering of proline genes promise ways forward for developing 'temperature-smart' future crop plants.

show abstract

Regulation of Osmotic Balance and Increased Antioxidant Activities under Heat Stress in Abelmoschus esculentus L. Triggered by Exogenous Proline Application

Cited by 26 publications

References 43 publications

Unraveling Heat Tolerance in Upland Cotton (Gossypium hirsutum L.) Using Univariate and Multivariate Analysis

Unraveling Heat Tolerance in Upland Cotton (Gossypium hirsutum L.) Using Univariate and Multivariate Analysis

Effects of Melatonin on Morphological Characteristics, Mineral Nutrition, Nitrogen Metabolism, and Energy Status in Alfalfa Under High-Nitrate Stress

Assessment of proline function in higher plants under extreme temperatures

Contact Info

Product

Resources

About