Regulation of osteogenesis of human amniotic mesenchymal stem cells by sodium butyrate

Fan, Xiaoran; Li, Lei; Ye, Zhaoyang; Zhou, Yan; Tan, Wen‐Song

doi:10.1002/cbin.10919

Cited by 14 publications

(12 citation statements)

References 42 publications

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“…Similarly, various pharmacologically active chemicals promote osteogenesis through activation of TAZ. These include TM-25659 (Jang et al, 2012), sodium butyrate (Fan et al, 2018), lipopolysaccharide (Xing et al, 2019), 1α,25-dihydroxyvitamin D3 (Ji et al, 2019), icariin (Wei et al, 2017;Ye et al, 2017), epicatechin gallate (ECG) (Byun et al, 2014b), phorbaketal A (Byun et al, 2012a), kaempferol (Byun et al, 2012b), and poncirin (Yoon et al, 2011).…”

Section: Role Of Yap/taz In Stem Cell Self-renewal and Maintenance Ofmentioning

confidence: 99%

Role of YAP/TAZ in Cell Lineage Fate Determination and Related Signaling Pathways

Heng

Zhang

Aubel

et al. 2020

Front. Cell Dev. Biol.

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The penultimate effectors of the Hippo signaling pathways YAP and TAZ, are transcriptional co-activator proteins that play key roles in many diverse biological processes, ranging from cell proliferation, tumorigenesis, mechanosensing and cell lineage fate determination, to wound healing and regeneration. In this review, we discuss the regulatory mechanisms by which YAP/TAZ control stem/progenitor cell differentiation into the various major lineages that are of interest to tissue engineering and regenerative medicine applications. Of particular interest is the key role of YAP/TAZ in maintaining the delicate balance between quiescence, self-renewal, proliferation and differentiation of endogenous adult stem cells within various tissues/organs during early development, normal homeostasis and regeneration/healing. Finally, we will consider how increasing knowledge of YAP/TAZ signaling might influence the trajectory of future progress in regenerative medicine.

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Section: Role Of Yap/taz In Stem Cell Self-renewal and Maintenance Ofmentioning

confidence: 99%

Role of YAP/TAZ in Cell Lineage Fate Determination and Related Signaling Pathways

Heng

Zhang

Aubel

et al. 2020

Front. Cell Dev. Biol.

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“…Inhibition of the activity of HDACs with specific or pan HDAC inhibitors, enhances osteogenic differentiation in detriment of adipogenic differentiation in many cellular models [6,30,40]. These effects could be a consequence of the change of the acetylation state in the N-term of histone H3 and/or H4.…”

Section: Discussionmentioning

confidence: 99%

“…It has been proposed that NaBu regulates osteogenesis in human amniotic mesenchymal stem cells and other stem cells [30]. We treated ASC with NaBu at a final concentration of 0.5 mM for three days before the induction of the osteogenic differentiation.…”

Section: Subcellular Localization Of Ack49/51 14-3-3 Proteins and Hbmentioning

confidence: 99%

14-3-3β paralog is inhibited by acetylation during differentiation to the osteogenic lineage.

López

Gojanovich

Uhart

et al. 2020

Preprint

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Background 14-3-3 protein family binds and regulate hundreds of serine/threonine phosphorylated proteins. Considered as redundant, ubiquitous and constantly expressed, this protein family was treated as an accessory for many signaling systems. Recently, specific biological functions were discovered for each of its seven paralogs in humans. Here, we studied the reversible inhibition by acetylation of its essential N- ε -lysine 49/51 residue during the osteogenic differentiation of human adipose-derived stem cells. Methods Human adipose-derived stem cells were obtained from healthy patients who underwent a dermolipectomy. The material was processed to obtain the ASCs from the stromal vascular fraction. Proteins of interest were analyzed by confocal microscopy and Western blot, and the expression level of selected proteins were modified by using lentivirus and specific shRNAs. Results We found that during the differentiation of ASC, the levels of 14-3-3 acK49/51 increased, showing that inhibition of 14-3-3 is necessary during this process. Among the 7 paralogs of this family, the inhibition by this posttranslational modification occurs mostly on the paralog β , located specifically in the nucleus, where 14-3-3 was described to bind to H3 histone and many transcription factors. Paralog specific inhibition by short hairpin RNA showed that silencing of 14-3-3 β gene, but not 14-3-3γ, increases significantly the osteogenic potential of the cells. Transdifferentiation from osteogenic to adipogenic linage of cell lines that were specifically silenced for 14-3-3 β o γ paralogs showed that 14-3-3b could be a negative regulator of the differentiation to the osteogenic lineage. Conclusion We showed that specifically 14-3-3 β is a negative regulator of osteogenesis. The levels of its inhibition by acetylation on lysine 51 is the key cellular mechanism to regulate it. The HBO1 acetyltransferase could be the enzyme responsible of this modification. Cells were this inhibition was emulated by decreasing the levels of expression of 14-3-3 β showed significant increased potential for the differentiation to the osteogenic lineage.

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“…The survival of MSCs is a significant point to be made about the use of epidrugs in promotion of MSC differentiation. The results of studies have shown that the viability of MSCs was affected by various doses of epidrugs; increased doses had a negative impact on MSC survival [ 117 , 119 , 132 ]. An evaluation of the effect of VPA and NaBu on MSCs showed a dose-dependent reduction in MSC proliferation.…”

Section: Epigenetic Modifiers Known As Epidrugsmentioning

confidence: 99%

Epidrugs: novel epigenetic regulators that open a new window for targeting osteoblast differentiation

et al. 2020

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Efficient osteogenic differentiation of mesenchymal stem cells (MSCs) is a critical step in the treatment of bone defects and skeletal disorders, which present challenges for cell-based therapy and regenerative medicine. Thus, it is necessary to understand the regulatory agents involved in osteogenesis. Epigenetic mechanisms are considered to be the primary mediators that regulate gene expression during MSC differentiation. In recent years, epigenetic enzyme inhibitors have been used as epidrugs in cancer therapy. A number of studies mentioned the role of epigenetic inhibitors in the regulation of gene expression patterns related to osteogenic differentiation. This review attempts to provide an overview of the key regulatory agents of osteogenesis: transcription factors, signaling pathways, and, especially, epigenetic mechanisms. In addition, we propose to introduce epigenetic enzyme inhibitors (epidrugs) and their applications as future therapeutic approaches for bone defect regeneration.

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Regulation of osteogenesis of human amniotic mesenchymal stem cells by sodium butyrate

Cited by 14 publications

References 42 publications

Role of YAP/TAZ in Cell Lineage Fate Determination and Related Signaling Pathways

Role of YAP/TAZ in Cell Lineage Fate Determination and Related Signaling Pathways

14-3-3β paralog is inhibited by acetylation during differentiation to the osteogenic lineage.

Epidrugs: novel epigenetic regulators that open a new window for targeting osteoblast differentiation

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