Regulation of oxidative stress inside living cells through polythiophene derivatives

Hu, Rong; Li, Sheng-Liang; Bai, Haotian; Liu, Libing; Lv, Fengting; Wang, Shu

doi:10.1016/j.cclet.2016.02.001

Cited by 10 publications

(10 citation statements)

References 31 publications

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“…In addition, the antioxidative ability of DHPs was used for creating more stable, light-sensitive DHP polythiophene derivatives (PTDHPs) as better alternatives to fluorescent probes used for cell imaging. These PTDHPs, via DHP groups, were found to regulate angiotensin-induced intracellular oxidative stress [ 36 ]. Recently, effective ROS radical scavenging was found also for some mitochondria-targeting DHPs [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidative 1,4-Dihydropyridine Derivatives Modulate Oxidative Stress and Growth of Human Osteoblast-Like Cells In Vitro

et al. 2018

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Oxidative stress has been implicated in pathophysiology of different human stress- and age-associated disorders, including osteoporosis for which antioxidants could be considered as therapeutic remedies as was suggested recently. The 1,4-dihydropyridine (DHP) derivatives are known for their pleiotropic activity, with some also acting as antioxidants. To find compounds with potential antioxidative activity, a group of 27 structurally diverse DHPs, as well as one pyridine compound, were studied. A group of 11 DHPs with 10-fold higher antioxidative potential than of uric acid, were further tested in cell model of human osteoblast-like cells. Short-term combined effects of DHPs and 50 µM H2O2 (1-h each), revealed better antioxidative potential of DHPs if administered before a stressor. Indirect 24-h effect of DHPs was evaluated in cells further exposed to mild oxidative stress conditions induced either by H2O2 or tert-butyl hydroperoxide (both 50 µM). Cell growth (viability and proliferation), generation of ROS and intracellular glutathione concentration were evaluated. The promotion of cell growth was highly dependent on the concentrations of DHPs used, type of stressor applied and treatment set-up. Thiocarbatone III-1, E2-134-1 III-4, Carbatone II-1, AV-153 IV-1, and Diethone I could be considered as therapeutic agents for osteoporosis although further research is needed to elucidate their bioactivity mechanisms, in particular in respect to signaling pathways involving 4-hydroxynoneal and related second messengers of free radicals.

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Section: Discussionmentioning

confidence: 99%

Antioxidative 1,4-Dihydropyridine Derivatives Modulate Oxidative Stress and Growth of Human Osteoblast-Like Cells In Vitro

et al. 2018

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“…In a recent work, Hu et al reported the use of polythiophene modified with dihydropyridine and demonstrated their antioxidative and anti-inflammatory properties in rat aortic endothelial cells [71].…”

Section: Exogenous Photostimulation: the Case Of Carbon-based Matementioning

confidence: 99%

Use of Exogenous and Endogenous Photomediators as Efficient ROS Modulation Tools: Results and Perspectives for Therapeutic Purposes

Antognazza

Aziz

Lodola

2019

Oxidative Medicine and Cellular Longevity

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Reactive Oxygen Species (ROS) play an essential dual role in living systems. Healthy levels of ROS modulate several signaling pathways, but at the same time, when they exceed normal physiological amounts, they work in the opposite direction, playing pivotal functions in the pathophysiology of multiple severe medical conditions (i.e., cancer, diabetes, neurodegenerative and cardiovascular diseases, and aging). Therefore, the research for methods to detect their levels via light-sensitive fluorescent probes has been extensively studied over the years. However, this is not the only link between light and ROS. In fact, the modulation of ROS mediated by light has been exploited already for a long time. In this review, we report the state of the art, as well as recent developments, in the field of photostimulation of oxidative stress, from photobiomodulation (PBM) mediated by naturally expressed light-sensitive proteins to the most recent optogenetic approaches, and finally, we describe the main methods of exogenous stimulation, in particular highlighting the new insights based on optically driven ROS modulation mediated by polymeric materials.

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“…[108109] Wang and co-workers synthesized 36 kDa PTs with tetraethyleneglycol side chains conjugated to the tyrosine-kinase inhibitor lapatinib and used the conjugates for intracellular transmembrane protein visualization. [110] The authors reported absorption and fluorescence emission maxima at 350 nm and 570 nm respectively with a quantum yield of 2.8 %. While maintaining cell viability, transmembrane staining of K-BR-3 and MCF-7 cell lines after 48 h incubation time was achieved.…”

Section: π-Conjugated Polymersmentioning

confidence: 99%

“…[111] Drawbacks of PTs, however, can lie within their nature as oxygen sensitizers, which makes them susceptible to photobleaching. [110]…”

Section: π-Conjugated Polymersmentioning

confidence: 99%

Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels

2021

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Cell analysis techniques like flow cytometry and fluorescence microscopy are widely used to explore cells in real-time and provide important insights into a variety of cellular processes. These techniques require bright and specific staining reagents to generate strong fluorescence signal and enable reliable readout, making the choice of fluorescent labels a key aspect of experimental design. Much research has been done in the field of fluorophore development to generate bright small-mole-cule dyes, fluorescent proteins, and emitting nanoparticles. However, it remains challenging to modify biomolecules with multiple emitters and avoid self-quenching of such fluorophores at the same time. Herein, we present advances in multimerization-based methods for the generation of fluorescent labels with high brightness and discuss their advantages and limitations in the context of flow cytometry and fluorescence microscopy applications.

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Regulation of oxidative stress inside living cells through polythiophene derivatives

Cited by 10 publications

References 31 publications

Antioxidative 1,4-Dihydropyridine Derivatives Modulate Oxidative Stress and Growth of Human Osteoblast-Like Cells In Vitro

Antioxidative 1,4-Dihydropyridine Derivatives Modulate Oxidative Stress and Growth of Human Osteoblast-Like Cells In Vitro

Use of Exogenous and Endogenous Photomediators as Efficient ROS Modulation Tools: Results and Perspectives for Therapeutic Purposes

Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels

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