Regulation of pituitary hormones and cell proliferation by components of the extracellular matrix

Páez-Pereda, Marcelo; Kuchenbauer, Florian; Arzt, Eduardo; Stalla, Günter

doi:10.1590/s0100-879x2005001000005

Cited by 65 publications

(43 citation statements)

References 57 publications

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“…In the current study, PTX was demonstrated to significantly suppress human oral cancer squamous cell line tea8113 proliferation in a time-and dose dependent manner (P<0.05). Tumor cells are characterized by evident cell proliferation capability, and PCNA is considered to be a hallmark of cell proliferation (20,21). According to the current study, when tea8113 cells were treated with PTX, the proliferation capability was significantly reduced as PCNA levels were obviously reduced.…”

Section: Discussionmentioning

confidence: 64%

Paclitaxel induces apoptosis and reduces proliferation by targeting epidermal growth factor receptor signaling pathway in oral cavity squamous cell carcinoma

Zhang

Wang

et al. 2015

Oncology Letters

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Abstract. Oral cavity cancer is common worldwide. Furthermore, the epidermal growth factor receptor (EGFR) signaling pathway is considered to be constitutively activated in oral cancers. Paclitaxel is widely accepted as an antitumor drug as it effectively inhibits the cell cycle. This study predominantly explores the possible molecule mechanism of paclitaxel on oral cancer treatment. Cell viability was first detected using an MTT assay. Cell apoptosis was examined by Hoechst staining and flow cytometry using an annexin-V and propidium iodide kit. Specific EGFR signaling pathways were further explored through western blot analysis. Abnormal protein expression levels were determined via immunofluoresence. Additionally, the protein levels of matrix metalloproteinase (MMP)-2 and 9 were determined using ELISA. Paclitaxel significantly inhibited oral cancer cell viability in a time-and dose-dependent manner. Paclitaxel also enhanced oral cancer cell apoptosis via increased Bim and Bid protein expression. Furthermore, paclitaxel was observed to inhibit oral cancer cell proliferation through increased MMP-2 and MMP-9 protein levels. Paclitaxel inhibited the growth of the oral cancer cell line, tea8113 malignant proliferation and enhanced tea8113 cell apoptosis through inhibiting the EGFR signaling pathway.

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Section: Discussionmentioning

confidence: 64%

Paclitaxel induces apoptosis and reduces proliferation by targeting epidermal growth factor receptor signaling pathway in oral cavity squamous cell carcinoma

Zhang

Wang

et al. 2015

Oncology Letters

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“…However, the mechanisms by which pituitary cells develop functional homotypic connections are far from clear. Different contact and signaling mechanisms in the pituitary include gap junctions, adherens junctions, integrin, and cadherin complexes (Soji et al 1997, Guerineau et al 1998, Ezzat & Asa 2005, Paez-Pereda et al 2005. In addition to gap junctions, physical contacts between FS cells are mediated by adherens junctions (Soji et al 1997), and we have shown that adherens junctions between GH cells actually resist physical dissociation, revealing the strong homotypic robustness of the GH cell network (Bonnefont et al 2005).…”

Section: Introductionmentioning

confidence: 75%

Characterization of adherens junction protein expression and localization in pituitary cell networks

Chauvet

El-Yandouzi²,

Mathieu³

et al. 2009

Journal of Endocrinology

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Our view of anterior pituitary organization has been altered with the recognition that folliculo-stellate (FS) and somatotroph cell populations form large-scale three-dimensional homotypic networks. This morphological cellular organization may optimize communication within the pituitary gland promoting coordinated pulsatile secretion adapted to physiological needs. The aim of this study was to identify the molecules involved in the formation and potential functional organization and/or signaling within these cell-cell networks. Here, we have focused on one class of cell adhesion molecules, the cadherins, since b-catenin has been detected in the GH cell network. We have characterized, by qPCR and immunohistochemistry, their cellular expression and distribution. We have also examined whether their expression could be modulated during pituitary tissue remodeling. The mouse anterior pituitary has a restricted and cell-type specific repertoire of cadherin expression: cadherin-11 is exclusively expressed in TSH cells; N-cadherin displays a ubiquitous expression pattern but with different levels of expression between endocrine cell types; E-cadherin is restricted to homotypic contacts between FS cells; while cadherin-18 is expressed both in somatotrophs and FS cells. Thus, each cell type presents a defined combinatorial expression of different subsets of cadherins. This cell-type specific cadherin expression profile emerges early during development and undergoes major changes during postnatal development. These results suggest the existence within the anterior pituitary of cell-cell contact signaling based on a defined pattern of cadherin expression, which may play a crucial role in cellular recognition during the formation and fate of pituitary cell homotypic networks.

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“…Altered levels of transforming growth factor alpha and beta protein families, epidermal growth factor, fibroblast growth factor family, bone morphogenetic protein 4 and IL-6/ gp130 family, have been observed in pituitary tumors (Jones et al 1994, Dworakowska & Grossman 2012, Perez-Castro et al 2012, Jiang & Zhang 2013. It was described that matrix metalloproteinase, secreted by pituitary cells, contribute to the control of cell proliferation during tumorigenesis also (Paez-Pereda et al 2005).…”

Section: Pathophysiological Role Of Il-6 In the Pituitarymentioning

confidence: 99%

Programmed cell senescence: role of IL-6 in the pituitary

Sapochnik

Fuertes

Arzt

2017

Journal of Molecular Endocrinology

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IL-6 is a pleiotropic cytokine with multiple pathophysiological functions. As a key factor of the senescence secretome, it can not only promote tumorigenesis and cell proliferation but also exert tumor suppressive functions, depending on the cellular context. IL-6, as do other cytokines, plays important roles in the function, growth and neuroendocrine responses of the anterior pituitary gland. The multiple actions of IL-6 on normal and adenomatous pituitary function, cell proliferation, angiogenesis and extracellular matrix remodeling indicate its importance in the regulation of the anterior pituitary. Pituitary tumors are mostly benign adenomas with low mitotic index and rarely became malignant. Premature senescence occurs in slow-growing benign tumors, like pituitary adenomas. The dual role of IL-6 in senescence and tumorigenesis is well represented in pituitary tumor development, as it has been demonstrated that effects of paracrine IL-6 may allow initial pituitary cell growth, whereas autocrine IL-6 in the same tumor triggers senescence and restrains aggressive growth and malignant transformation. IL-6 is instrumental in promotion and maintenance of the senescence program in pituitary adenomas.

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Regulation of pituitary hormones and cell proliferation by components of the extracellular matrix

Cited by 65 publications

References 57 publications

Paclitaxel induces apoptosis and reduces proliferation by targeting epidermal growth factor receptor signaling pathway in oral cavity squamous cell carcinoma

Paclitaxel induces apoptosis and reduces proliferation by targeting epidermal growth factor receptor signaling pathway in oral cavity squamous cell carcinoma

Characterization of adherens junction protein expression and localization in pituitary cell networks

Programmed cell senescence: role of IL-6 in the pituitary

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