Regulation of Plant Stem Cell Quiescence by a Brassinosteroid Signaling Module

Vilarrasa-Blasi, Josep; González-García, Mary-Paz; Frigola, David; Fàbregas, Norma; Alexiou, Konstantinos G.; López‐Bigas, Núria; Rivas, Susana; Jauneau, Alain; Lohmann, Jan U.; Benfey, Philip N.; Ibañes, Marta; Caño‐Delgado, Ana I.

doi:10.1016/j.devcel.2014.05.020

Cited by 178 publications

(192 citation statements)

References 48 publications

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“…Arabidopsis thaliana accession Columbia (Col-0) was used along with previously described mutants: atg5-1 (Thompson et al, 2005), atg7-2 (Chung et al, 2010), bri1-301 (Li and Nam, 2002), bes1-D (Vilarrasa-Blasi et al, 2015; Yin et al, 2002), bin2-1D (Li et al, 2001), bin2-3 bil1 bil2 (Yan et al, 2009). Well characterized DSK2 RNAi lines (Lin et al, 2011) were used in this study.…”

Section: Methodsmentioning

confidence: 99%

Selective Autophagy of BES1 Mediated by DSK2 Balances Plant Growth and Survival

et al. 2017

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SUMMARY Plants encounter a variety of stresses and must fine-tune their growth and stress-response programs to best suit their environment. BES1 functions as a master regulator in the brassinosteroid (BR) pathway that promotes plant growth. Here, we show that BES1 interacts with the ubiquitin receptor protein DSK2 and is targeted to the autophagy pathway during stress via the interaction of DSK2 with ATG8, a ubiquitin-like protein directing autophagosome formation and cargo recruitment. Additionally, DSK2 is phosphorylated by the GSK3-like kinase BIN2, a negative regulator in the BR pathway. BIN2 phosphorylation of DSK2 flanking its ATG8 interacting motifs (AIMs) promotes DSK2-ATG8 interaction, thereby targeting BES1 for degradation. Accordingly, loss-of-function dsk2 mutants accumulate BES1, have altered global gene expression profiles, and have compromised stress responses. Our results thus reveal that plants coordinate growth and stress responses by integrating BR and autophagy pathways and identify the molecular basis of this crosstalk.

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Section: Methodsmentioning

confidence: 99%

Selective Autophagy of BES1 Mediated by DSK2 Balances Plant Growth and Survival

et al. 2017

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“…In fact, recent studies have demonstrated that BR signaling pathway is required to regulate hypocotyl cell expansion (Gallego-Bartolomé et al 2012;Li et al 2012;Oh et al 2012), as well as, to promote the transition from meristematic cells to primordial cells in the shoot (Oh et al 2011;Zhiponova et al 2013). In the root apex, BRs are further involved in controlling root growth, both coordinating root meristem size and also root cell elongation Heyman et al 2013;Vilarrasa-Blasi et al 2014;Vragovic et al 2015). In all of these physiological processes BES1/BZR1 complex plays a pivotal role, interacting with several TFs in order to connect other signalling pathways.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of the BZR transcription factor family and its expression in response to abiotic stresses in Zea mays L.

et al. 2017

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Brassinosteroids (BRs) are plant specific steroidal hormones that play diverse roles in regulating a broad spectrum of plant growth and developmental processes, as well as, in responding to various biotic and abiotic stresses. Extensive research over the years has established stress-impact-mitigating role of BRs and associated compounds in different plants exposed to various abiotic and biotic stresses, suggesting the idea that they may act as immunomodulators, thus opening new approaches for plant resistance against hazardous environmental conditions. In this research the characterization of the transcriptional response of 11 transcription factors (TFs) belonging to BRASSINAZOLE-RESISTANT 1 (BZR1) TF family of Zea mays L. was analyzed in seedlings subjected to different stress conditions. Being important regulators of the BR synthesis, BZR TFs might have stress resistance related activities. However, no stress resistance related functional study of BZR TFs has been reported in maize so far. In silico analyses of the selected 11 TFs validated the features of their protein domains, where the highest degree of similarity observed with recognized BZR TFs of rice and Sorghum bicolor. Additionally, we investigated the organ-specific expression of 11 ZmBZR in maize seedlings. Five of them did not show any transcript accumulation, suggesting that ZmBZR expression might be regulated in a manner dependent on plant developmental stage. For the remaining six ZmBZR, their ubiquitous expression in the whole plant indicates they could function as growth regulators during maize development. More importantly, in response to various stress conditions, the spatial transcript accumulation of all ZmBZR varies along the plant. All six ZmBZR showed up-regulation against N starvation, hypoxia and salt stress. On the contrary, heat stress clearly down-regulated gene expression of all ZmBZR analysed. Consistently with the expression results, the distribution of stress-related cis-acting elements in the promoter of these genes inferred that the maize BZR TFs might play some roles in regulating the expression of the corresponding genes in response to multifarious stresses. In conclusion, these data reveal that BZR TFs have stress signaling activity in maize, in addition to their confirmed role in regulating plant physiology and morphology.

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“…Many hormones affect the cell cycle and cell expansion directly or indirectly, including brassinosteroids in the root [87][88][89] and gibberellins, abscisic acid, and ethylene in various development contexts [7,8]; however, cytokinin and auxin are the major hormonal regulators of core cell cycle components [8]. For many plant cells, determination is accompanied by endoreduplication, cell polyploidisation, and cessation of cell proliferation.…”

Section: Cytokinins and Auxins And The Cell Cyclementioning

confidence: 99%