Regulation of plasma acute-phase protein and albumin levels in the liver of scalded rats

Ševaljević, Ljiljana; Ivanović‐Matić, Svetlana; Petrović, M.; Glibetić, Marija; Pantelić, D; Poznanović, Goran

doi:10.1042/bj2580663

Cited by 34 publications

(20 citation statements)

References 26 publications

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“…This process reaches its peak in 12-24 h. Albumin has clearly been shown to leak from the intravascular space during at least the first 6-18 h after injury [17,18]. Besides, examination of the livers of burned animals reveals that the messenger RNA for albumin is decreased in response to injury [19,20], a response that is associated with the increase in acute phase reactants. After burn shock, therefore, serum albumin levels would continue to decrease because of decreased production and ongoing losses through the wound.…”

Section: Discussionmentioning

confidence: 99%

Impact of Burn Size and Initial Serum Albumin Level on Acute Renal Failure Occurring in Major Burn

Kim¹,

Oh²,

Yoon³

et al. 2002

Am J Nephrol

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Background: Acute renal failure (ARF) is not a rare occurrence in severe burns and is an important complication leading to an increase in mortality. The severity of the burn is largely determined by the burn size, and severe burns are likely to cause enough loss of extracellular fluid and albumin from plasma volume to produce shock and hypoalbuminemia. Hypothesis: We hypothesized that initial serum albumin level may be useful as an indicator of prognosis and severity of injury in burned patients. Methods: The clinical characteristics of 147 adult patients with second- and third-degree burns covering 30% or more of their body surface area were analyzed retrospectively. Logistic regression was used to estimate the relative risks of ARF and mortality associated with the larger burn size and the lower serum albumin level at admission. Results: Mean burned body surface was 60.0 ± 21.8% (range 30–100%). Twenty-eight (19.0%) out of 147 patients experienced ARF, defined as a serum creatinine ≧2 mg/dl, during the admission. The patients with ARF had larger burn size (79.5 ± 15.4 vs. 55.3 ± 20.5%, p < 0.0001) and lower serum albumin concentration at admission (1.92 ± 0.66 vs. 2.48 ± 0.82 g/dl, p < 0.0005) compared with those without ARF. All patients with ARF expired, whereas 29.4% (35/119) of the patients without ARF died. The burn size ≧65% was associated with a risk of ARF that was 9.9 times and with a risk of death that was 14.2 times as high as that for the burn size <65%. The initial serum albumin level <2.5 g/dl was associated with a risk of death that was 2.7 times as high as that for the initial serum albumin level ≧2.5 g/dl. Conclusions: When major burns are complicated by ARF, the mortality rate increases significantly. Burn size is an independent predictor of ARF occurring in major burns. Initially depressed serum albumin level is associated with an increase in mortality in the major burn patients.

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Section: Discussionmentioning

confidence: 99%

Impact of Burn Size and Initial Serum Albumin Level on Acute Renal Failure Occurring in Major Burn

Kim¹,

Oh²,

Yoon³

et al. 2002

Am J Nephrol

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“…The control animals were routinely injected with pyrogen-free saline. The animals were killed 24 h after the initial treatment, when maximal transcription of the rat haptoglobin gene is established (13). Until their death, the rats were kept at constant temperature, humidity, and dark/light intervals.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, by bringing together speci c DNA sequences and transactivator proteins, the nuclear matrix organizes and stabilizes the DNA in a speci c 3-dimensional conformation that consequently determines tissue-speci c gene expression (6). In the course of studying the dynamics of nuclear matrix-DNA interactions, we observed that the increased rate of transcription of the rat haptoglobin gene during the acute-phase (AP) response (13) was accompanied by an increased binding of its anking 5 0 region to the nuclear matrix (14). This was mediated by a MAR-like, 38-base-long adenine tract lying 147 bp upstream from the cis-element (¡ 165/¡ 56).…”

Section: Introductionmentioning

confidence: 99%

Identification of Nuclear Matrix and Associated Proteins that Bind the Haptoglobin Gene Cis‐Element

et al. 1999

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SummaryTo identify the major nuclear matrix proteins that bind to the rat haptoglobin gene cis-element, we isolated a soluble nuclear matrix protein fraction and analysed it by gel retardation. Two major DNA-binding proteins exhibiting different types of protein-DNA interactions were detected: a DNA sequence-speci c 32-kDa isoform of transcription factor C/EBP¯, and a nuclear matrix protein p55 that bound to the DNA nonspeci cally. During increased transcription of the haptoglobin gene in the course of the acutephase reaction, the DNA-binding af nities and concentrations of these proteins in the soluble nuclear matrix fraction were increased. These data lend further evidence that the nuclear matrix is an active support structure that localizes gene regulatory proteins and participates in transcriptional regulation. IUBMB Life, 48: 277-282, 1999

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“…After acute systemic tissue injury, transcription of the Hp gene, mRNA concentration, Hp protein synthesis, and plasma concentration are increased three-to sixfold (1,33,47). The principal acute-phase mediators for hepatic Hp regulation in human cell systems have been identified as interleukin-6 (IL-6) and glucocorticoids (7,12,18,37).…”

mentioning

confidence: 99%

Structure, hormonal regulation, and identification of the interleukin-6- and dexamethasone-responsive element of the rat haptoglobin gene.

Marinkovic¹,

Baumann²

1990

Mol. Cell. Biol.

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Hepatic expression of the haptoglobin (Hp) gene in mammalian species is stimulated severalfold during an acute-phase reaction. To identify the molecular mechanism responsible for this regulation, the single-copy rat Hp gene has been isolated. The genomic sequences showed a high degree of homology with the primate Hp gene. Activity of the rat Hp gene was increased in cultured liver cells by interleukin-1 (IL-1), IL-6, and glucocorticoids. The genomic Hp gene sequence spanning from -6500 to +6500, when transiently introduced into human hepatoma (HepG2) cells, directed IL-6- and dexamethasone-stimulated expression of rat Hp mRNA and protein. No response to IL-1 was detected, suggesting that the corresponding regulatory element(s) might lie outside of the tested gene sequences. An IL-6- and dexamethasone-responsive element has been localized to the promoter proximal region -146 to -55. Although the nucleotide sequences of this rat Hp gene region showed substantial divergence from that of the human gene, analysis of sequential 5' and 3' deletion constructs indicated an arrangement of functional IL-6 response elements in the rat Hp promoter sequence comparable to that of the human homolog. The magnitude of IL-6 regulation through the rat Hp gene promoter was severalfold lower than that of the human Hp gene. The reduced activity could be ascribed to a single-base difference in an otherwise conserved sequence corresponding to an active element in the human gene. The IL-6 response of the rat Hp element was improved severalfold by substituting that base with the human nucleotide.

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Regulation of plasma acute-phase protein and albumin levels in the liver of scalded rats

Cited by 34 publications

References 26 publications

Impact of Burn Size and Initial Serum Albumin Level on Acute Renal Failure Occurring in Major Burn

Impact of Burn Size and Initial Serum Albumin Level on Acute Renal Failure Occurring in Major Burn

Identification of Nuclear Matrix and Associated Proteins that Bind the Haptoglobin Gene Cis‐Element

Structure, hormonal regulation, and identification of the interleukin-6- and dexamethasone-responsive element of the rat haptoglobin gene.

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