Regulation of Plasminogen Activator in Rat Endometrial Stromal Cells: The Role of Prostaglandin E21

Zhang, X; Shu, M.A.; Ross, Howard; Kennedy, T. G.

doi:10.1095/biolreprod54.5.1046

Cited by 19 publications

(12 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The present study demonstrated that uPA mRNA is present in decidual cells in vivo. The increase in its abundance on Day 7 of pregnancy is consistent with the increase in uPA secretion in vitro by stromal cells at the corresponding stage of decidualization [10]. In situ hybridization studies confirmed the localization of uPA mRNA to the decidual cells on Day 7, consistent with findings from in situ hybridization studies of the mouse decidua at similar stages of pregnancy [18,19].…”

Section: Discussionsupporting

confidence: 80%

“…Urokinase-type PA is secreted by rat endometrial stromal cells during decidualization in vitro, and this secretion is controlled by de novo-synthesized prostaglandin E 2 that acts to increase steady-state levels of uPA mRNA [10]. It is necessary to determine whether uPA mRNA is present in decidual cells in vivo because uPA gene expression in some cell types can be induced by artifacts resulting from in vitro manipulations [9].…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have demonstrated that rat endometrial stromal cells from uteri sensitized for the decidual cell reaction secrete uPA in vitro when cultured under conditions that allow the cells to decidualize. Prostaglandin E 2 , a mediator of decidualization, regulates the in vitro secretion of uPA, at least partially, by regulating the steady-state level of uPA mRNA [10]. The present study was designed to determine whether the levels of uPA mRNA in the rat endometrium change during decidualization in vivo as they do in vitro.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Presence of Urokinase Plasminogen Activator and Plasminogen Activator Inhibitor-1 Messenger Ribonucleic Acids in Rat Endometrium during Decidualization in Vivo1

Wang

Kennedy

Zhang

1996

Biology of Reproduction

View full text Add to dashboard Cite

Rat endometrial stromal cells undergoing decidualization in vitro secrete urokinase-type plasminogen activator (uPA), and this secretion is regulated by prostaglandin E2. The present study was undertaken to determine whether uPA and plasminogen activator inhibitor-1 (PAI-1) mRNAs are expressed in vivo in the decidua of pregnant rats and in the deciduoma of "pseudopregnant" rats. Total RNA was prepared from nondecidualized and decidualized endometrial tissues at various stages of early pregnancy and examined by Northern blot analysis using specific cDNA probes for rat uPA and PAI-1. There was little uPA mRNA in the endometrium during the first 5 days of pregnancy (Day 1 = the presence of sperm in the vagina). A high level of uPA mRNA was detected on Day 7, and it declined thereafter. There was a gradual increase in PAI-1 mRNA in the decidua from Day 7 of pregnancy, reaching a peak level on Day 15 when the decidua was transformed into the maternal placenta. (RNA was not analyzed beyond Day 15 of pregnancy in this study.) In situ hybridization studies verified that uPA mRNA was present in the decidua adjacent to the implanting embryo on Day 7. Plasminogen activator inhibitor-1 mRNA was scattered in the decidualized endometrium, but greater amounts of PAI-1 mRNA were found in the fetal tissue on Day 10 of pregnancy. Northern blot analysis of RNA from the deciduoma produced in ovariectomized, steroid-treated rats by intrauterine injection of oil demonstrated a similar temporal pattern of expression of uPA mRNA; i.e., the level of uPA mRNA was highest on Day 7 and decreased thereafter. The level of PAI-1 mRNA in deciduoma was not detectable by Northern blot technique during the first 10 days of pseudopregnancy. These findings confirm that uPA mRNA is present in vivo in rat decidual cells, independent of the presence of a conceptus. By contrast, the level of PAI-1 mRNA in the uterus is probably influenced by the presence of the conceptus.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Presence of Urokinase Plasminogen Activator and Plasminogen Activator Inhibitor-1 Messenger Ribonucleic Acids in Rat Endometrium during Decidualization in Vivo1

Wang

Kennedy

Zhang

1996

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…The PAs cleave plasminogen to plasmin, which degrades ECM components such as fibronectin and laminin, and are activators of MMPs; their natural inhibitors are the plasminogen activator inhibitors. Transcripts for uPA, tPA, and plasminogen activator inhibitor-1 have all been detected within the uterus during decidualization both in vivo and in vitro (50,51). PGE 2 and EGF increase levels for uPA, but not tPA (51,52), providing additional evidence that EGF increases the proteolytic activity of stromal cells in vitro.…”

Section: Discussionmentioning

confidence: 88%

Epidermal Growth Factor and Basic Fibroblast Growth Factor Increase the Production of Matrix Metalloproteinases during in Vitro Decidualization of Rat Endometrial Stromal Cells

Nuttall

Kennedy

2000

Endocrinology

View full text Add to dashboard Cite

Numerous growth factors are involved in mediating proliferation and differentiation of endometrial stromal cells during decidualization. During this period, the extracellular matrix of the endometrium undergoes extensive remodeling. We tested the hypothesis that epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and transforming growth factor-␤ regulate expression of matrix metalloproteinases (MMPs) and their inhibitors, tissue inhibitors of metalloproteinases (TIMPs), during decidualization. Stromal cells were isolated from uteri hormonally sensitized to undergo decidualization and were cultured in the absence or presence of a growth factor. Using substrate-gel electrophoresis with gelatin as the substrate, we detected activity for gelatinase A and B, and collagenase-3, and using casein as a substrate, we detected activity for stromelysin-1. Increasing concentrations of EGF and bFGF resulted in increased activity of gelatinase B, collagenase-3, and stromelysin-1. Northern blot analyses revealed that EGF and bFGF also increased messenger RNA levels for these MMPs. There was no effect of these growth factors on gelatinase or TIMP-1, -2, and -3, nor was there an effect of transforming growth factor-␤ on any MMP or TIMP examined. These data demonstrate that EGF and bFGF increase levels of proteolytic enzymes produced by endometrial stromal cells undergoing decidualization in vitro while having no effect on their inhibitors. (Endocrinology 141: 629 -636, 2000) D URING EMBRYO implantation in rodents, the stromal fibroblast cells of the endometrium proliferate and differentiate into decidual cells, a process that ultimately results in the formation of the maternal component of the placenta (1). Several lines of evidence indicate that this process, in addition to being regulated by estrogen and progesterone, is also regulated by several locally produced growth factors. There is increased binding of epidermal growth factor (EGF) to the uterus during decidualization (2), and this factor is able to induce embryo implantation in the absence of estrogen (3). The messenger RNA (mRNA) for basic fibroblast growth factor (bFGF) is present in the uterus during early pregnancy (4), and its levels increase substantially during decidualization, specifically within the mesometrial decidual cells (5). The expression of bFGF in the uterus is regulated by progesterone, and bFGF is involved in controlling both stromal cell proliferation (6) and angiogenesis (5). The mRNA for transforming growth factor-␤ (TGF␤) is also present within the uterus during pregnancy and is localized to the luminal and glandular epithelial cells during early decidualization and to macrophages during midgestation (7).During decidualization, the extracellular matrix (ECM) of the endometrium undergoes extensive remodeling, whereby the fibrillar collagen underlying the undifferentiated stromal cells becomes less fibrous due to the removal of several collagen components from the matrix (8). Subsequently, the newly differentiated decidu...

show abstract

“…Implantation processes include blastocystuterine attachment and stromal decidualization with vascular permeability changes (Rankin et al 1979, Kennedy 1983. Indeed, Kennedy's group and others have showed that PGE 2 plays a major role in the implantation and decidualization in the rodent uterus (Zhang et al 1996). COX-2 and PGE 2 have also been shown to be important in the baboon endometrium at the time of implantation (for a review, see Fazleabas et al (1999)).…”

Section: Introductionmentioning

confidence: 99%

Regulation of prostaglandin D synthase and prostacyclin synthase in the endometrium of cyclic, pregnant, and pseudopregnant rats and their regulation by sex steroids

Kengni

St-Louis

Parent

et al. 2007

Journal of Endocrinology

View full text Add to dashboard Cite

Prostaglandins (PGs) are critical regulators of a number of reproductive processes. To date, the presence and regulation of PGS in the rat endometrium have not yet been described. The objective of the present study was to investigate the expression of PGD synthase (PGDS) and prostacyclin synthase (PGIS) in the endometrium. Endometrial proteins and tissues were collected from cyclic non-pregnant, pregnant, and steroid-induced pseudopregnant rats. PGIS and PGDS were detected in the endometrium of cyclic, pregnant, and pseudopregnant rats but were not influenced by the estrous cycle. During early pregnancy, PGIS was significantly higher at day 5 and was gradually decreased from day 5 . 5 to 6 . 5. Later during pregnancy, PGIS was maximal on day 12 and gradually decreased to the end of pregnancy. PGDS expression was high during early and was maximal at the end of pregnancy. During pseudopregnancy, PGDS and PGIS were increased in a time-dependent manner and were maximal at day 5. Immunohistochemical analysis revealed that PGDS and PGIS were found in luminal as well as glandular epithelial cells and in stroma during late pregnancy. We also found a significant increase of PGD 2 serum metabolite at days 21 and 22 of pregnancy. During steroidinduced pseudopregnancy, PGI 2 serum metabolite was increased in a time-dependent manner and was maximal at day 7. These results suggest that PGDS and PGIS are present and could be regulated by steroids in the rat uterus during pregnancy, and that the endometrium could be a significant source of PGD 2 and PGI 2 at specific times during pregnancy.

show abstract

Regulation of Plasminogen Activator in Rat Endometrial Stromal Cells: The Role of Prostaglandin E21

Cited by 19 publications

References 28 publications

Presence of Urokinase Plasminogen Activator and Plasminogen Activator Inhibitor-1 Messenger Ribonucleic Acids in Rat Endometrium during Decidualization in Vivo1

Presence of Urokinase Plasminogen Activator and Plasminogen Activator Inhibitor-1 Messenger Ribonucleic Acids in Rat Endometrium during Decidualization in Vivo1

Epidermal Growth Factor and Basic Fibroblast Growth Factor Increase the Production of Matrix Metalloproteinases during in Vitro Decidualization of Rat Endometrial Stromal Cells

Regulation of prostaglandin D synthase and prostacyclin synthase in the endometrium of cyclic, pregnant, and pseudopregnant rats and their regulation by sex steroids

Contact Info

Product

Resources

About