Regulation of Polar Auxin Transport by AtPIN1 in
            <i>Arabidopsis</i>
            Vascular Tissue

Gälweiler, Leo; Guan, Changhui; Müller, Andreas; Wisman, Ellen; Mendgen, Kurt; Yephremov, Alexander; Palme, Klaus

doi:10.1126/science.282.5397.2226

Cited by 1,402 publications

(1,095 citation statements)

References 31 publications

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“…The Bjpin1 cDNA has a length of 2557 nucleotides with an open reading frame present between nucleotides 126 (ATG) and 2042 (TGA). The predicted protein contained 639 amino acid residues and had a molecular mass of 7 0 kDa, similar to that of previously reported AtPIN1 [21] and AtPIN2/EIR1 [22]. The calculated isoelectric point of BjPIN1 was 8.88.…”

Section: Cloning Of Bjpin1 From B Junceasupporting

confidence: 81%

“…The Atpin1 gene, isolated through En-tagging method, was found to encode a protein with similarity to bacterial and eukaryotic carrier proteins. AtPIN1 protein was detected at the basal end of auxin transport-competent cells in vascular tissues [21]. These studies strongly suggested that AtPIN1 might act as a transmembrane component of the auxin efflux carrier and play a role in auxin transport from shoot apex to root tissue in stems.…”

Section: Introductionmentioning

confidence: 74%

“…[29]. The nucleotide sequence of Atpin1 [21] and two other Atpin1 homologous sequences (Accession Nos. AC002311, gene T26J12.…”

Section: Bacteria and Plantsmentioning

confidence: 99%

“…Further proof of AUX1 as an auxin influx carrier was provided by the work of Yamamoto and Yamamoto (1998) [19]. A breakthrough in the study of polar auxin transport has been marked with the cloning of two genes, Atpin1 and Atpin2, which encoded presumptive auxin efflux carriers [20][21][22][23][24]. Pin-formed inflorescence and decreased polar auxin transport activity of the Arabidopsis pin1 mutant indicated that the primary function of the AtPIN1 protein was for polar auxin transport in the inflorescence axis [25].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and functional analysis of a Brassica juncea gene encoding a component of auxin efflux carrier

Chen

et al. 2002

Cell Res

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Polar auxin transport plays a divergent role in plant growth and developmental processes including root and embryo development, vascular pattern formation and cell elongation. Recently isolated Arabidopsis pin gene family was believed to encode a component of auxin efflux carrier . Based on the Arabidopsis pin1 sequence we have isolated a Brassica juncea cDNA (designated Bjpin1), which encoded a 70-kDa putative auxin efflux carrier. Deduced BjPIN1 shared 65% identities at protein level with AtPIN1 and was highly homologous to other putative PIN proteins of Arabidopsis (with highest homology to AtPIN3). Hydrophobic analysis showed similar structures between BjPIN1 and AtPIN proteins. Presence of 6 exons (varying in size between 65 bp and 1229 bp) and 5 introns (sizes between 89 bp and 463 bp) in the genomic fragment was revealed by comparing the genomic and cDNA sequences. Northern blot analysis indicated that Bjpin1 was expressed in most of the tissues tested, with a relatively higher level of transcript in flowers and a lower level in root tissues. Promoter-reporter gene fusion studies further revealed the expression of Bjpin1 in the mature pollen grains, young seeds, root tip, leaf vascular tissue and trace bundle, stem epidermis, cortex and vascular cells. BjPIN1 was localized on the plasma membrane as demonstrated through fusion expression of green fluorescent protein (GFP). Auxin efflux carrier activity was elevated in transgenic Arabidopsis expressing BjPIN1.

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Section: Cloning Of Bjpin1 From B Junceasupporting

confidence: 81%

Section: Introductionmentioning

confidence: 74%

“…[29]. The nucleotide sequence of Atpin1 [21] and two other Atpin1 homologous sequences (Accession Nos. AC002311, gene T26J12.…”

Section: Bacteria and Plantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and functional analysis of a Brassica juncea gene encoding a component of auxin efflux carrier

Chen

et al. 2002

Cell Res

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“…Recently several pin genes have been cloned by Dr. Palme s group in Max-Planck Institute of Plant Breeding in Köln, and one of them (pin1) is correlated to the mutant pin1-1 described by Okada. Due to the PIN protein predicted from pin1 sequence having typical membrane domain, they consider that PIN1 may be an auxin transporter [22]. According to the fact that protein kinase inhibitors could reduce the auxin polar transport activity in etiolated pea seedlings, it was suggested that inhibition of protein phosphorylation could lead to the hindrance of the auxin-exporting function of NPA receptors [23].…”

Section: Discussionmentioning

confidence: 99%

Foliar modifications induced by inhibition of polar transport of auxin

Wang

et al. 1999

Cell Res

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The effects of auxin polar transport inhibitors,9-hydroxyfluorene-9-carboxylic acid (HFCA); 2, 3, 5-triiodobenzoic acid (TIBA) and trans-cinnamic acid (CA) on leaf pattern formation were investigated with shoots formed from cultured leaf explants of tobacco and cultured pedicel explants of Orychophragmus violaceus, and the seedlings of tobacco and Brassica chinensis. Although the effective concentration varies with the inhibitors used, all of the inhibitors induced the formation of trumpet-shaped and/or fused leaves. The frequency of trumpet-shaped leaf formation was related to the concentration of inhibitors in the medium. Histological observation of tobacco seedlings showed that there was only one main vascular bundle and several minor vascular bundles in normal leaves of the control, but there were several vascular bundles of more or less the same size in the trumpet-shaped leaves of treated ones. These results indicated that auxin polar transport played an important role on bilateral symmetry of leaf growth.

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Actin cytoskeleton in plants: From transport networks to signaling networks

Volkmann

Baluška

1999

Microsc. Res. Tech.

144

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The plant actin cytoskeleton is characterized by a high diversity in regard to gene families, isoforms, and degree of polymerization. In addition to the most abundant F‐actin assemblies like filaments and their bundles, G‐actin obviously assembles in the form of actin oligomers composed of a few actin molecules which can be extensively cross‐linked into complex dynamic meshworks. The role of the actomyosin complex as a force generating system — based on principles operating as in muscle cells — is clearly established for long‐range mass transport in large algal cells and specialized cell types of higher plants. Extended F‐actin networks, mainly composed of F‐actin bundles, are the structural basis for this cytoplasmic streaming of high velocities On the other hand, evidence is accumulating that delicate meshworks built of short F‐actin oligomers are critical for events occurring at the plasma membrane, e.g., actin interventions into activities of ion channels and hormone carriers, signaling pathways based on phospholipids, and exo‐ and endocytotic processes. These unique F‐actin arrays, constructed by polymerization‐depolymerization processes propelled via synergistic actions of actin‐binding proteins such as profilin and actin depolymerizing factor (ADF)/cofilin are supposed to be engaged in diverse aspects of plant morphogenesis. Finally, rapid rearrangements of F‐actin meshworks interconnecting endocellular membranes turn out to be especially important for perception‐signaling purposes of plant cells, e.g., in association with guard cell movements, mechano‐ and gravity‐sensing, plant host–pathogen interactions, and wound‐healing. Microsc. Res. Tech. 47:135–154, 1999. © 1999 Wiley‐Liss, Inc.

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Regulation of Polar Auxin Transport by AtPIN1 in Arabidopsis Vascular Tissue

Cited by 1,402 publications

References 31 publications

Isolation and functional analysis of a Brassica juncea gene encoding a component of auxin efflux carrier

Isolation and functional analysis of a Brassica juncea gene encoding a component of auxin efflux carrier

Foliar modifications induced by inhibition of polar transport of auxin

Actin cytoskeleton in plants: From transport networks to signaling networks

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