Regulation of preprocambial cell state acquisition by auxin signaling in<i>Arabidopsis</i>leaves

Donner, Tyler J.; Sherr, Ira; Scarpella, Enrico

doi:10.1242/dev.037028

Cited by 264 publications

(351 citation statements)

References 86 publications

Supporting

Mentioning

330

Contrasting

Unclassified

Order By: Relevance

“…Loss of A2 alone [pS15-2E (DA2)::LUC] drastically reduced auxin induction of LUC activity, demonstrating that A2 is acting as an essential AuxRE in the SAUR15 promoter. Recently, another element with the same sequence as A2 (CA-GACA) was found to act as a bona fide AuxRE in the unrelated ATHB8 promoter (Donner et al, 2009). Consistent with this finding, we observed that loss of A2 alone [pS15-2E(DA2)::LUC] drastically reduced auxin induction of the reporter, demonstrating that A2 (CAGACA) but not A5 (GAGACA) is the AuxRE for SAUR15.…”

Section: Auxin and Brassinosteroid Induction Of Saur15 Requires Two Csupporting

confidence: 87%

See 1 more Smart Citation

Bipartite Promoter Element Required for Auxin Response

Walcher

Nemhauser

2011

Plant Physiology

View full text Add to dashboard Cite

Multiple mechanisms have been described for coordination of responses to the plant hormones auxin and brassinosteroids (Zhang et al., 2009). One unexplained phenomenon is the reliance of the auxin transcriptional response on a functional brassinosteroid pathway. In this study, we used luciferase reporters to interrogate the promoter of SMALL AUXIN-UP RNA15 (SAUR15), a well-characterized auxin and brassinosteroid early response gene in Arabidopsis (Arabidopsis thaliana). After identifying a minimal region sufficient for auxin response, we targeted predicted cis-regulatory elements contained within this sequence and found a critical subset required for hormone response. Specifically, reporter sensitivity to auxin treatment required two elements: a Hormone Up at Dawn (HUD)-type E-box and an AuxRE-related TGTCT element. Reporter response to brassinosteroid treatment relied on the same two elements. Consistent with these findings, the transcription factors BRASSINOSTEROID INSENSITIVE1-EMS SUPPESSOR1 and MONOPTEROS (MP)/ AUXIN RESPONSE FACTOR5 (ARF5) showed enhanced binding to the critical promoter region containing these elements. Treatment with auxin or brassinosteroids could enhance binding of either transcription factor, and brassinosteroid enhancement of MP/ARF5 binding required an intact HUD element. Conservation of clustered HUD elements and AuxRE-related sequences in promoters of putative SAUR15 orthologs in a number of flowering plant species, in combination with evidence for statistically significant clustering of these elements across all Arabidopsis promoters, provided further evidence of the functional importance of coordinated transcription factor binding.

show abstract

Section: Auxin and Brassinosteroid Induction Of Saur15 Requires Two Csupporting

confidence: 87%

“…Previous studies suggested that MP/ARF5 regulates SAUR15 expression (Schlereth et al, 2010) and can bind to A2-like elements (Donner et al, 2009). We performed ChIP assays on pMP::MP:GFP seedlings using primers flanking the critical minimal region where we observed BES1 binding.…”

Section: Treatment With Either Auxin or Brassinosteroids Canmentioning

confidence: 99%

Bipartite Promoter Element Required for Auxin Response

Walcher

Nemhauser

2011

Plant Physiology

View full text Add to dashboard Cite

show abstract

“…The pATHB8-GUS marker for the provascular cell state revealed a defective venation patterning in the elo mutants, with disconnected primary loops and reduced secondary and tertiary veins, which is typical for an altered auxin distribution (Fig. 3 D and E) (22).…”

Section: Gfp-elo3 Protein Localization In Euchromatic Regions In Intementioning

confidence: 90%

“…On the basis of the elo phenotypes and the transcriptome, we identified a number of genes with reduced expression levels (Table S3) as putative targets of Elongator, i.e., the SHY2/IAA3 and BDL/ IAA12 repressors, the ATHB8 auxin response gene (22), the LAX2 influx carrier, the PIN4 efflux carrier, and the AP2/ERF ethylene response gene. The down-regulation of these genes in the elo mutants was verified with quantitative (q)PCR (Fig.…”

Section: Hormonal Misregulation Might Contribute To the Pleiotropicmentioning

confidence: 99%

Plant Elongator regulates auxin-related genes during RNA polymerase II transcription elongation

Nelissen

Groeve

Fleury

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

115

192

View full text Add to dashboard Cite

In eukaryotes, transcription of protein-encoding genes is strongly regulated by posttranslational modifications of histones that affect the accessibility of the DNA by RNA polymerase II (RNAPII). The Elongator complex was originally identified in yeast as a histone acetyltransferase (HAT) complex that activates RNAPII-mediated transcription. In Arabidopsis thaliana, the Elongator mutants elo1, elo2, and elo3 with decreased leaf and primary root growth due to reduced cell proliferation identified homologs of components of the yeast Elongator complex, Elp4, Elp1, and Elp3, respectively. Here we show that the Elongator complex was purified from plant cell cultures as a six-component complex. The role of plant Elongator in transcription elongation was supported by colocalization of the HAT enzyme, ELO3, with euchromatin and the phosphorylated form of RNAPII, and reduced histone H3 lysine 14 acetylation at the coding region of the SHORT HYPOCOTYL 2 auxin repressor and the LAX2 auxin influx carrier gene with reduced expression levels in the elo3 mutant. Additional auxin-related genes were down-regulated in the transcriptome of elo mutants but not targeted by the Elongator HAT activity showing specificity in target gene selection. Biological relevance was apparent by auxin-related phenotypes and marker gene analysis. Ethylene and jasmonic acid signaling and abiotic stress responses were up-regulated in the elo transcriptome and might contribute to the pleiotropic elo phenotype. Thus, although the structure of Elongator and its substrate are conserved, target gene selection has diverged, showing that auxin signaling and influx are under chromatin control.Arabidopsis | chromatin | histone acetyltranferase complex | SHY2

show abstract

“…We aimed to identify novel MP target genes involved in the earliest events of embryonic root meristem initiation in the globular-stage embryo. As most MP target genes are expected to be activated by MP (17,(26)(27)(28)(29), we focused our analysis on the 145 down-regulated genes in the globular-stage embryo (SI Appendix, Table S1). Among these genes, ∼25% are transcription factors (36 of 145 genes) and 27 were also downregulated in heart-stage embryos (Fig.…”

Section: Mpdependent Stringent Targetsmentioning

confidence: 99%

Auxin response cell-autonomously controls ground tissue initiation in the early Arabidopsis embryo

Möller

Hove

Xiang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Significance Higher plants are built from three major tissue types: epidermis, ground tissue, and vascular tissue. Each of these differentiates into several functionally distinct cell types. Although identity switches for the different cell types within the major three tissues have been identified, mechanisms that trigger the initiation of the three tissues themselves have remained obscure. Auxin response, in particular the auxin-dependent transcription factor MONOPTEROS (MP), plays a critical role in Arabidopsis embryonic root initiation. In our study, we identify a set of embryonic MP target genes and show that MP acts as a very first regulator of ground tissue initiation. Moreover, our data provide a framework for the simultaneous formation of multiple cell types by the same transcriptional regulator.

show abstract

Regulation of preprocambial cell state acquisition by auxin signaling inArabidopsisleaves

Cited by 264 publications

References 86 publications

Bipartite Promoter Element Required for Auxin Response

Bipartite Promoter Element Required for Auxin Response

Plant Elongator regulates auxin-related genes during RNA polymerase II transcription elongation

Auxin response cell-autonomously controls ground tissue initiation in the early Arabidopsis embryo

Contact Info

Product

Resources

About