Regulation of Procoagulant Factors in Mononuclear Phagocytes

Dean, Roger T.; Leoni, Patricio Clark Di; Rossi, Bartira C.

doi:10.1159/000215099

Cited by 6 publications

(5 citation statements)

References 9 publications

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“…chronic inflammatory diseases, delayed type hypersensitivity, rejec tion of organ transplants or cancer [reviewed in ref. 5], [3,17,18] including adherence [19]. This could be confirmed in our present study using hydrophobic Teflon as the cultivation sub strate.…”

Section: Discussionsupporting

confidence: 85%

Interferon-Gamma-lnduced Expression of Tissue Factor Activity during Human Monocyte to Macrophage Maturation

Scheibenbogen

Moser²,

Krause³

et al. 1992

Pathophysiol Haemos Thromb

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The expression of tissue factor (TF) during differentiation of human monocytes (MO) to macrophages (MAC) in vitro is investigated in this study. In MO, TF activity can be induced by the addition of lipopolysaccharides (LPS) as detected by Northern blot analysis and measured functionally as procoagulant activity (PCA), while IFNγ alone has only marginal stimulatory activity. During in vitro differentiation of MO into MAC, however, there is a steady increase in IFNγ-induced PCA with a maximum on day 7. While MO during the first 2 days in culture are more responsive to LPS, IFNγ becomes the prominent stimulus for mature MAC. The response to IFNγ is rapid with a peak within 6-8 h and a subsequent downregulation to baseline activity within 24 h. Our results demonstrate that IFNγ can effectively induce TF in human MAC and that its expression is developmentally regulated during MO to MAC maturation in vitro.

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Section: Discussionsupporting

confidence: 85%

Interferon-Gamma-lnduced Expression of Tissue Factor Activity during Human Monocyte to Macrophage Maturation

Scheibenbogen

Moser²,

Krause³

et al. 1992

Pathophysiol Haemos Thromb

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“…Once associated with a vegetation monocytes can be induced to generate thromboplastin by bacteria, bacterial products, ly*phokines, or complement (28,29).Phagocytosis of bacteria on the vegetational surface could be a stimulus since phagocytosis of S. sanguis and ,S. epidermidis by human as well as rabbit monocytes (30) in vitro was shown to increase thromboplastic activity in these cells. Since infected vegeiations contain more monocytes than sterile vegetations do (3), the elevated procoagulant activity of infected vegetation could be due to activation of the monocytes, to a larger population of these cells, or most probably a combination of these two.…”

Section: Discussionmentioning

confidence: 99%

Procoagulant Activity of Endocardial Vegetations and Blood Monocytes in Rabbits with Streptococcus sanguis Endocarditis

et al. 1989

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SummaryTo investigate activation of the coagulation system in bacterial endocarditis, we determined the procoagulant activity of blood monocytes isolated from rabbits with Streptococcus sanguisinfected or sterile catheter-induced endocardial vegetations. This activity was determined directly after isolation from the peripheral blood and after stimulation in vitro by either endotoxin or by phagocytosis of S. sanguis. The procoagulant activity of the vegetations of these rabbits was also determined.The procoagulant activity of blood monocytes of rabbits with S. sanguis endocarditis was found to be similar to the activity of monocytes of rabbits with sterile vegetations, both at the time of isolation and after stimulation in vitro by exposure to endotoxin or phagocytosis of bacteria. The procoagulant activity of infected vegetations was significantly higher than that of sterile vegetations. We conclude that in bacterial endocarditis the coagulation system is activated locally at the site of the vegetation. Triggering probably occurs by thromboplastin generated by monocytes activated by phagocytosis of bacteria on the vegetational surface.

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“…MPCA is generated by a number of foreign stimuli (e.g., bacterial lipopolysaccharide (LPS), lectins, allogeneic cells) and endogenous activators such as macrophage-procoagulant-inducing factor (MPIF), IFNy in combination with a second signal, complement components and immune complexes (Dean et al, 1984;Ryan and Geczy, 1987;Geczy and Jones, 1988). Fibach et al (1985) showed that some chemotherapeutic drugs induced procoagulant activity on the promyelocytic cell line HL60.…”

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confidence: 99%

Induction of macrophage procoagulant expression by cisplatin, daunorubicin and doxorubicin

Wheeler

Geczy

1990

Intl Journal of Cancer

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Cisplatin, doxorubicin and daunorubicin (drugs which intercalate with DNA) influenced the membrane-bound procoagulant potential of murine thioglycollate-induced peritoneal exudate (TG-PEC) macrophages and the monocytoid cell line WEHI 265, whereas the antimetabolites 5-fluorouracil and methotrexate had no effect. Enhanced procoagulant was not caused by non-specific toxicity of these agents. Cisplatin directly increased the procoagulant expressed on WEHI 265 cells, whereas MPCA on TG-PEC was enhanced only when cisplatin was combined with a second stimulant, either bacterial lipopolysaccharide (LPS) or interferon (IFN gamma). WEHI 265 cells failed to respond to the anthracycline drugs, either alone or in combination with LPS, whereas they enhanced the IFN gamma response. Doxorubicin and daunorubicin increased the LPS response of TG-PEC by approximately 4-fold and the IFN gamma response by approximately 10-fold. Pulsing experiments suggested that the anthracyclines enhanced procoagulant expression by a mechanism different from cisplatin. Daunorubicin primed TG-PEC within 4 hr to respond to low levels of LPS, whereas either LPS or cisplatin primed these cells to respond to cisplatin or LPS respectively. Furthermore, the procoagulant expressed by TG-PEC stimulated by LPS/cisplatin had properties of tissue factor (TF: 50% total activity) and Factor VIIa (50% total procoagulant)-like activities, whereas the predominant procoagulant on LPS/anthracycline activated TG-PEC was TF-like (70% total activity) with weak Factor VIIa and prothrombinase-like properties.

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Regulation of Procoagulant Factors in Mononuclear Phagocytes

Cited by 6 publications

References 9 publications

Interferon-Gamma-lnduced Expression of Tissue Factor Activity during Human Monocyte to Macrophage Maturation

Interferon-Gamma-lnduced Expression of Tissue Factor Activity during Human Monocyte to Macrophage Maturation

Procoagulant Activity of Endocardial Vegetations and Blood Monocytes in Rabbits with Streptococcus sanguis Endocarditis

Induction of macrophage procoagulant expression by cisplatin, daunorubicin and doxorubicin

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