Regulation of production of the blue pigment indigoidine by the pseudo γ-butyrolactone receptor FarR2 in Streptomyces lavendulae FRI-5

Kurniawan, Yohanes Novi; Kitani, Shigeru; Iida, Aya; Maeda, Akiko; Nijeholt, Jelger Lycklama a; Lee, Yong Jik; Nihira, Takuya

doi:10.1016/j.jbiosc.2015.08.013

Cited by 14 publications

(11 citation statements)

References 27 publications

(28 reference statements)

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“…Since farR3 and farR4 can be transcribed both as monocistronic and bicistronic mRNA, it appears that farR3 is also a target of FarA 299 . FarR2 is a pseudo6GBL receptor that positively regulates the production of indigoidine, but negatively regulates the expression of the far regulatory genes in the regulatory island, including the expression of farX 300 . Similarly, FarR3 positively regulates the production of indigoidine 299 , but in both cases the control is most likely indirect 300,301 .…”

Section: Gbl Receptors and Antibiotic Production In Other Streptomycetesmentioning

confidence: 99%

“…FarR2 is a pseudo6GBL receptor that positively regulates the production of indigoidine, but negatively regulates the expression of the far regulatory genes in the regulatory island, including the expression of farX 300 . Similarly, FarR3 positively regulates the production of indigoidine 299 , but in both cases the control is most likely indirect 300,301 . The SARP regulator FarR4 represses IM2 biosynthesis 299 .…”

Section: Gbl Receptors and Antibiotic Production In Other Streptomycetesmentioning

confidence: 99%

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Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era

et al. 2018

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Covering: 2000 to 2018 The antimicrobial activity of many of their natural products has brought prominence to the Streptomycetaceae, a family of Gram-positive bacteria that inhabit both soil and aquatic sediments. In the natural environment, antimicrobial compounds are likely to limit the growth of competitors, thereby offering a selective advantage to the producer, in particular when nutrients become limited and the developmental programme leading to spores commences. The study of the control of this secondary metabolism continues to offer insights into its integration with a complex lifecycle that takes multiple cues from the environment and primary metabolism. Such information can then be harnessed to devise laboratory screening conditions to discover compounds with new or improved clinical value. Here we provide an update of the review we published in NPR in 2011. Besides providing the essential background, we focus on recent developments in our understanding of the underlying regulatory networks, ecological triggers of natural product biosynthesis, contributions from comparative genomics and approaches to awaken the biosynthesis of otherwise silent or cryptic natural products. In addition, we highlight recent discoveries on the control of antibiotic production in other Actinobacteria, which have gained considerable attention since the start of the genomics revolution. New technologies that have the potential to produce a step change in our understanding of the regulation of secondary metabolism are also described.

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Section: Gbl Receptors and Antibiotic Production In Other Streptomycetesmentioning

confidence: 99%

Section: Gbl Receptors and Antibiotic Production In Other Streptomycetesmentioning

confidence: 99%

Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era

et al. 2018

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“…The avaR2 gene (sav_3702), located 3.45 Mb from the ave cluster, encodes a 23.1-kDa TetR family transcriptional regulator classified as a pseudoreceptor (Kurniawan et al, 2015). avaR2 is clustered with the other two GBL receptor homologous genes, avaR1 (sav_3705) and avaR3 (sav_3703), in the middle of the S. avermitilis chromosome (Fig.…”

Section: Avar2 Negatively Regulates Avermectin Production and Cell Grmentioning

confidence: 99%

“…AvaR3 promotes avermectin production , but it is unclear whether this regulatory effect is direct or indirect. Phylogenetic tree analysis clearly indicated that the AvaR2 protein belongs to the branch of pseudo GBL receptors, rather than the branch of genuine GBL receptors (Kurniawan et al, 2015). AvaR2 is a close homologue of the pseudo GBL receptors ScbR2 (43% identity) and JadR2 (53% identity).…”

Section: Introductionmentioning

confidence: 98%

AvaR2, a pseudo γ‐butyrolactone receptor homologue from Streptomyces avermitilis, is a pleiotropic repressor of avermectin and avenolide biosynthesis and cell growth

Zhu

Sun

Liu

et al. 2016

Molecular Microbiology

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Avermectins produced by Streptomyces avermitilis are effective anthelmintic agents. The autoregulatory signalling molecule that triggers avermectin biosynthesis is a novel butenolide-type molecule, avenolide, rather than common γ-butyrolactones (GBLs). We identified AvaR2, a pseudo GBL receptor homologue, as an important repressor of avermectin and avenolide biosynthesis and cell growth. AvaR2 directly repressed transcription of aveR (the ave cluster-situated activator gene), aco (a key gene for avenolide biosynthesis), its own gene (avaR2) and two other GBL receptor homologous genes (avaR1 and avaR3) by binding to their promoter regions. The aveR promoter had the highest affinity for AvaR2. A consensus 18 bp ARE (autoregulatory element)-like sequence was found in the AvaR2-binding regions of these five target genes. Eleven novel AvaR2 targets were identified, including genes involved in primary metabolism, ribosomal protein synthesis, and stress responses. AvaR2 bound and responded to endogenous avenolide and exogenous antibiotics jadomycin B (JadB) and aminoglycosides to modulate its DNA-binding activity. Our findings help to clarify the roles of pseudo GBL receptors as pleiotropic regulators and as receptors for new type autoregulator and exogenous antibiotic signal. A pseudo GBL receptor-mediated antibiotic signalling transduction system may be a common strategy that facilitates Streptomyces interspecies communication and survival in complex environments.

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“…In Serratia, the antibiotic carbapenem and the pigment prodigiosin were synthesized under quorum sensing control while in Monascus ruber, red pigment and citrinin production could be affected by amino acids in medium at the same culture time (5,6). In some actinomycetes, pigment and antibiotic were biosynthesized by polyketide syntheses and regulated by some autoregulators in the same growth phase (7,8). In the fermentation of Streptomyces hygroscopicus 5008 e the strain used in industrial production of Val-A, a fitting equation based on the relationship between spectral absorption (SA) value of the pigment and Val-A concentration in broth may be established.…”

mentioning

confidence: 99%

Novel spectrophotometric approach for determination of validamycin A in fermentation of Streptomyces hygroscopicus

Wei

Feng

Jiang

et al. 2016

Journal of Bioscience and Bioengineering

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Regulation of production of the blue pigment indigoidine by the pseudo γ-butyrolactone receptor FarR2 in Streptomyces lavendulae FRI-5

Cited by 14 publications

References 27 publications

Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era

Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era

AvaR2, a pseudo γ‐butyrolactone receptor homologue from Streptomyces avermitilis, is a pleiotropic repressor of avermectin and avenolide biosynthesis and cell growth

Novel spectrophotometric approach for determination of validamycin A in fermentation of Streptomyces hygroscopicus

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