Regulation of progesterone and prostaglandin F2α production in the CL

Diaz, Francisco J.; Anderson, L. E.; Wu, Yuh-Lin; Rabot, Aline; Tsai, Shaw-Jenq; Wiltbank, Milo C.

doi:10.1016/s0303-7207(02)00056-4

Cited by 132 publications

(93 citation statements)

References 127 publications

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“…However, luteal cells, in the course of cellular differentiation in vivo and in vitro, undergo a progressive hypertrophy (4,45,46). Given the selective induction of the FP receptor in ovarian cells following ovulation and the high levels of prostaglandins present during early CL development (1,2,47), it is plausible that mTOR/4EBP1/S6K1 signaling participates in the hypertrophy/differentiation of the luteal cell. In keeping with this theme, Alam et al (48) recently suggested that gonadotropin stimulated mTOR signaling may play a role in the differentiation of rat granulosa cells to the large luteal cell type.…”

Section: Discussionmentioning

confidence: 99%

AKT-independent Phosphorylation of TSC2 and Activation of mTOR and Ribosomal Protein S6 Kinase Signaling by Prostaglandin F2α

Arvisais

Romanelli

Hou

et al. 2006

Journal of Biological Chemistry

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The corpus luteum (CL)2 is a transient endocrine gland derived from an ovulated follicle within the ovary. The steroidogenic cells of the CL produce progesterone, a prerequisite for normal maintenance of pregnancy in mammals. In the event of pregnancy, the CL retains its role in progesterone synthesis in support of early pregnancy. In the absence of pregnancy luteolysis or corpus luteum regression occurs, a physiologic process associated with a reduction in progesterone secretion (functional regression) followed by death of endothelial and steroidogenic cells (structural regression) (1-4). Prostaglandin F2␣ (PGF2␣) was identified as a luteolytic factor over 35 years ago (5, 6). Recent genetic studies in mice lacking the PGF2␣ receptor (FP) further highlight the role for PGF2␣ in CL regression. Mice lacking FP receptors experience defects in CL regression and consequently parturition does not occur because of the maintenance of progesterone secretion at the end of pregnancy (7).The FP receptor is a member of the large class of heterotrimeric G-protein-coupled receptors (GPCRs) (8) and is present on the surface of steroidogenic luteal cells. PGF2␣ binding to its G q -coupled receptor results in activation of phospholipase ␤ (PLC␤) and consequent generation of the second messengers; diacylglycerol and inositol trisphosphate (9). The resultant increase in protein kinase C (PKC) activity in luteal cells contributes to the activation of other downstream protein kinases. PGF2␣ stimulates the activity of the extracellular signal-regulated kinase (Erk) family of mitogen-activated protein kinases (MAPK) through a mechanism that involves the PKC-dependent phosphorylation and activation of Raf (10, 11). These events result in the induction of early response genes that code for transcription factors that in turn, alter the synthesis of proteins that regulate progesterone synthesis (12-14). Little is understood regarding other signaling mechanisms initiated by PGF2␣ that account for the actions of PGF2␣ in the CL.The mammalian target of rapamycin (mTOR) protein is a key regulator of protein translation via mechanisms involving the phosphorylation of the translation regulator eukaryotic initiation factor 4E (eIF4E) -binding protein (4EBP1) and the 70-kDa ribosomal protein S6 protein kinase 1 (S6K1) (15,16 receptor; GPCR, G-protein-coupled receptor; mTOR, mammalian target of rapamycin; m 7 G, 7-methyl-guanosine; PI3K, phosphatidylinositol-3-kinase; PGF2␣, prostaglandin F2␣; S6K1, p70 ribosomal S6 kinase; TSC2, tuberous sclerosis complex 2; FBS, fetal bovine serum; FCS, fetal calf serum; JNK, c-Jun N-terminal kinase; PMA, phorbol 12-myristate 13-acetate; PKC, protein kinase C.

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Section: Discussionmentioning

confidence: 99%

AKT-independent Phosphorylation of TSC2 and Activation of mTOR and Ribosomal Protein S6 Kinase Signaling by Prostaglandin F2α

Arvisais

Romanelli

Hou

et al. 2006

Journal of Biological Chemistry

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“…The receptor population has not been examined during the late luteal phase in the dairy cow, only in stage I and stage II/III (Wiltbank et al 1995). The intraluteal concentrations of PGF 2a may be quite high during luteolysis and may reach concentrations at which occupancy of the low affinity site is significant (Diaz et al 2002). This might explain the presence of low affinity FPr during the stage IV luteal phase.…”

Section: Discussionmentioning

confidence: 99%

“…The corpus luteum (CL) is a transient ovarian endocrine structure that plays a pivotal role in the control of reproduction in mammals (for reviews see Niswender & Nett 1994, Niswender et al 2000, Diaz et al 2002. In ruminants, CL regression is set in motion by the uterine secretion of prostaglandin F 2a (PGF 2a ) (reviewed in McCracken et al 1999, Niswender et al 2000.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the aims of the present study were to clone cDNA for buffalo FPr and to evaluate the FPr expression and protein levels during different stages of CL development in the buffalo cow. Since StAR gene expression could well be a key determinant of luteal progesterone biosynthesis (for review see Diaz et al 2002) during the normal oestrous cycle, its expression was studied as a marker for the functional status of the CL.…”

Section: Introductionmentioning

confidence: 99%

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Cloning of a buffalo (Bubalus bubalis) prostaglandin F2α receptor: changes in its expression and concentration in the buffalo cow corpus luteum

Verma-Kumar¹,

Srinivas²,

Muraly³

et al. 2004

Reproduction

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Acting primarily through its specific G protein-coupled receptor termed FPr, prostaglandin (PG) F 2a induces regression of the corpus luteum (CL) at the end of a non-fertile oestrous cycle. This study was aimed at cloning a full-length cDNA for FPr and determining its expression and protein concentrations during different stages of CL development in the water buffalo. Serum progesterone and StAR expression were determined to establish temporal relationships between indices of steroidogenesis and changes in FPr expression at different stages of CL development. In contrast to the dairy cow, the stage IV CL (day 20 of the oestrous cycle) did not appear to be functionally regressed in the buffalo.

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“…The bovine CL is a transient organ with an average lifetime of approximately 17 -18 days and undergoes extreme change throughout its life span in terms of size, structure and function [2,3]. The fate of the CL is principally determined by either the luteolytic action of uterine produced prostaglandin F2a (PGF2a), which induces regression of the CL [4] or the luteotrophic action of anterior pituitary produced luteinizing hormone (LH), which is responsible for the maintenance and steroidogenic activity of the CL [5]. In addition, there is growing evidence implicating the IGF system, angiogenic factors and immune cells in the development, maintenance and regression of the CL [6,7].…”

Section: Introductionmentioning

confidence: 99%

Analysis of gene expression in the bovine corpus luteum through generation and characterisation of 960 ESTs

Casey

Fitzpatrick

McInerney

et al. 2004

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expres

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To gain new insights into gene identity and gene expression in the bovine corpus luteum (CL) a directionally cloned CL cDNA library was constructed, screened with a total CL cDNA probe and clones representing abundant and rare mRNA transcripts isolated. The 5V-terminal DNA sequence of 960 cDNA clones, composed of 192 abundant and 768 rare mRNA transcripts was determined and clustered into 351 non-redundant expressed sequence tag (EST) groups. Bioinformatic analysis revealed that 309 (88%) of the ESTs showed significant homology to existing sequences in the protein and nucleotide public databases. Several previously unidentified bovine genes encoding proteins associated with key aspects of CL function including extracellular matrix remodelling, lipid metabolism/steroid biosynthesis and apoptosis, were identified. Forty-two (12%) of the ESTs showed homology with human or with other uncharacterised ESTs, some of these were abundantly expressed and may therefore play an important role in primary CL function. Tissue-specificity and temporal CL gene expression of selected clones previously unidentified in bovine CL tissue was also examined. The most interesting finds indicated that mRNA encoding squalene epoxidase was constitutively expressed in CL tissue throughout the oestrous cycle and 7-fold down-regulated ( P < 0.05) in late luteal tissue, concomitant with the disappearance of systemic progesterone, suggesting that de novo cholesterol biosynthesis plays an important role in steroidogenesis. The mRNA encoding the growth factor, insulin-like growth factor-binding protein-related protein 1 (IGFBP-rP1), remained constant during the oestrous cycle and was 1.8-fold up-regulated ( P < 0.05) in late luteal tissue implying a role in CL regression. D

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Regulation of progesterone and prostaglandin F2α production in the CL

Cited by 132 publications

References 127 publications

AKT-independent Phosphorylation of TSC2 and Activation of mTOR and Ribosomal Protein S6 Kinase Signaling by Prostaglandin F2α

AKT-independent Phosphorylation of TSC2 and Activation of mTOR and Ribosomal Protein S6 Kinase Signaling by Prostaglandin F2α

Cloning of a buffalo (Bubalus bubalis) prostaglandin F2α receptor: changes in its expression and concentration in the buffalo cow corpus luteum

Analysis of gene expression in the bovine corpus luteum through generation and characterisation of 960 ESTs

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