Regulation of Prostaglandin Synthesis by Interleukin-1β in Cultured Bovine Luteal Cells1

Townson, David H.; Pate, Joy L.

doi:10.1095/biolreprod51.3.480

Cited by 54 publications

(36 citation statements)

References 36 publications

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“…In bovine luteal cells (Townson & Pate 1994), IL1B appears to require the stimulation of PL and/or the metabolism of AA for the production of PGs. Moreover, in the bovine uterus, cyclooxygenase-2 (COX2) converts AA to PGH2 (Asselin et al 1997), which is then converted by PGE synthase (PGES) and PGF synthase (PGFS) to PGE2 and PGF2a respectively (Madore et al 2003, Parent & Fortier 2005.…”

Section: Introductionmentioning

confidence: 99%

Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells

Tanikawa¹,

Lee²,

Watanabe³

et al. 2008

Journal of Endocrinology

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Interleukin-1 (IL1) has been shown to be a potent stimulator of prostaglandin (PG) production in bovine endometrium. The aim of the present study was to determine the cell types in the endometrium (epithelial or stromal cells) responsible for the secretion of PGE2 and PGF2a in response to IL1A, and the intracellular mechanisms of IL1A action. Cultured bovine epithelial and stromal cells were exposed to IL1A or IL1B (0 . 006-3 . 0 nM) for 24 h. IL1A and IL1B dose-dependently stimulated PGE2 and PGF2a production in the stromal cells, but not in the epithelial cells. The stimulatory effect of IL1A (0 . 06-3 . 0 nM) on PG production was greater than that of IL1B.The stimulatory actions of IL1A on PG production was augmented by supplementing arachidonic acid (AA). When the stromal cells were incubated with IL1A and inhibitors of phospholipase (PL) C or PLA2 (1 mM; anthranilic acid), only PLA2 inhibitor completely stopped the stimulatory action of IL1A on PG production. Moreover, a specific cyclooxygenase-2 (COX2) inhibitor blocked the stimulatory effect of IL1A on PG production. IL1A (0 . 06 nM) promoted COX2 and microsomal PGE synthase-1 (PGES1) gene and its protein expression. The expression of COX1, PGES2, PGES3, and PGF synthase (PGFS) mRNA was not affected by IL1A in the stromal cells. The overall results indicate that 1) the target of IL1A and IL1B for stimulating both PGE2 and PGF2a production is the stromal cells, 2) IL1A is a far more potent stimulator than IL1B on PG production in stromal cells, 3) the stimulatory effect of IL1A on PG production is mediated via the activation of PLA2 and COX2, and (4) IL1A induced PG production by increasing expressions of COX2 and PGES1 mRNAs and their proteins in bovine stromal cells.

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Section: Introductionmentioning

confidence: 99%

Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells

Tanikawa¹,

Lee²,

Watanabe³

et al. 2008

Journal of Endocrinology

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“…Third, some components of the intraovarian IL-1 system (e.g., IL-1 ␤ and the type I IL-1 receptor) appear to be expressed in vivo, mainly during a narrow periovulatory window (6)(7)(8)(9)(10)(11)(12). Fourth, IL-1 ␤ has been shown to induce a host of ovulation-associated phenomena in vitro, such as the promotion of prostaglandin production (13)(14)(15)(16), the stimulation of hyaluronic acid biosynthesis (17), the induction of collagenase activity (18), and the activation of nitric oxide synthase activity (19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

The midcycle increase in ovarian glucose uptake is associated with enhanced expression of glucose transporter 3. Possible role for interleukin-1, a putative intermediary in the ovulatory process.

et al. 1997

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This study characterizes the rat ovary as a site of hormonally dependent glucose transporter (Glut) expression, and explores the potential role of interleukin (IL)-1, a putative intermediary in the ovulatory process, in this regard. Molecular probing throughout a simulated estrous cycle revealed a significant surge in ovarian Glut3 (but not Glut1) expression at the time of ovulation. Treatment of cultured whole ovarian dispersates from immature rats with IL-1 ␤ resulted in upregulation of the relative abundance of the Glut1 (4.5-fold) and Glut3 (3.5-fold) proteins as determined by Western blot analysis. Other members of the Glut family (i.e., Gluts 2, 4, and 5) remained undetectable. The ability of IL-1 to upregulate Glut1 and Glut3 transcripts proved time-, dose-, nitric oxide-, and protein biosynthesis-dependent but glucose independent. Other ovarian agonists (i.e., TNF ␣ , IGF-I, interferon-␥ , and insulin) were without effect. Taken together, our findings establish the mammalian ovary as a site of cyclically determined Glut1 and Glut3 expression, and disclose the ability of IL-1 to induce the ovarian expression as well as translation of Glut1 and Glut3 (but not of Gluts 2, 4, or 5). Our observations also establish IL-1 as the first known regulator of Glut3, the most efficient Glut known to date. In so doing, IL-1, a putative component of the ovulatory process, may be acting to meet the increased metabolic demands imposed on the growing follicle and the ovulated cumulus-enclosed oocyte. ( J. Clin. Invest. 1997. 99:2274-2283.)

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“…Since IL-1 stimulates ovarian PGE 2 levels (Kokia et al 1992, Brannstrom et al 1993, Townson & Pate 1994, we examined whether this effect was blocked by TGF 1 (Fig. 3).…”

Section: Tgf 1 Suppresses Il-1 -Stimulated Prostaglandin E 2 Accumulamentioning

confidence: 99%

“…It is possible that IL-1 , a promoter of inflammation, and TGF , a promotor of fibrosis and healing, might play complementary roles in the ovulatory process. IL-1 induces a number of inflammatory-like phenomena which have been shown to be associated with ovulation (Kokia et al 1992, Hurwitz et al 1993, Ben-Shlomo et al 1994b, Townson & Pate 1994. In contrast, the function of TGF in ovarian physiology is uncertain (Shull & Doetschman 1994), but evidence suggests that TGF may act as a general regulator of follicle cell differentiation (Dodson & Schomberg 1987).…”

Section: Figurementioning

confidence: 99%

“…IL-1 synergizes with luteinizing hormone in bringing about ovulation (Brannstrom et al 1993, Takehara et al 1994, and components of the intraovarian IL-1 system (IL-1 and the type I IL-1 receptor (IL-1R)) are only expressed during a narrow periovulatory window (Hurwitz et al 1991, Simon et al 1994, Wang et al 1997. Furthermore, IL-1 is capable of promoting a number of ovulation-associated phenomena in cultured ovarian cells, including an increase in prostaglandin production (Kokia et al 1992, Townson & Pate 1994, stimulation of nitric oxide synthesis (Ben-Shlomo et al 1994b), induction of collagenase activity (Hurwitz et al 1993) and a metabolic shift towards aerobic glycolysis as evidenced by increased lactate accumulation and glucose consumption (Ben-Shlomo et al 1997). Additionally, IL-1 appears to enhance the production of mRNA transcripts encoding for itself (Hurwitz et al 1991).…”

Section: Introductionmentioning

confidence: 99%

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Transforming growth factor-beta1 is a potent inhibitor of interleukin-1beta action in whole ovarian dispersates

Derman

Kol²,

Ben‐Shlomo³

et al. 1999

Journal of Endocrinology

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Transforming growth factor 1 (TGF 1 ) acts as an inhibitor of the actions of interleukin-1 (IL-1 ) in various organ systems. In order better to understand the interØactions between these polypeptides in the ovary, we evaluated the effect of TGF 1 co-treatment on various IL-1 -mediated actions in cultures of whole ovarian dispersates. Treatment with IL-1 enhanced media accumulation of nitrites (4·8-fold), prostaglandin E 2 (PGE 2 , 3·9-fold) and lactate (2·0-fold), and enhanced glucose consumption (2·1-fold). Treatment with TGF 1 alone did not significantly affect any of these parameters. However, the addition of TGF 1 inhibited IL-1 -stimulated nitrite (100%), PGE 2 (44%) and lactate (78%) accumulation and inhibited IL-1 -stimulated glucose consumption (74%) in a dose-dependent manner. The addition of TGF 1 also suppressed the steady-state levels of IL-1 -stimulated IL-1 , type I IL-1 receptor and IL-1 receptor antagonist transcripts (98, 67 and 83% inhibition respectively). These data suggest that TGF 1 is capable of inhibiting several IL-1 -stimulated endpoints. Since IL-1 has been identified as a possible proinflammatory mediator of ovulation and TGF has been implicated as a promotor of fibrosis and healing, we speculate that IL-1 and TGF might play antagonistic roles in the normal ovulatory sequence.

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Regulation of Prostaglandin Synthesis by Interleukin-1β in Cultured Bovine Luteal Cells1

Cited by 54 publications

References 36 publications

Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells

Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells

The midcycle increase in ovarian glucose uptake is associated with enhanced expression of glucose transporter 3. Possible role for interleukin-1, a putative intermediary in the ovulatory process.

Transforming growth factor-beta1 is a potent inhibitor of interleukin-1beta action in whole ovarian dispersates

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