Regulation of protease inhibitors and plant defense

Koiwa, Hisashi; Bressan, Ray A.; Hasegawa, Paul M.

doi:10.1016/s1360-1385(97)90052-2

Cited by 425 publications

(284 citation statements)

References 31 publications

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“…The induction of both ABA and proteinase inhibitor was detected distal to the site of hormone application in an ABA-deficient mutant, showing the mobility of the hormone in plants (Koiwa et al, 1997).…”

Section: Systemic Signals Induced By Herbivory and Woundingmentioning

confidence: 98%

“…seeds, bulbs and leaves. In some cases, the inhibitors are developmentally regulated, while in other cases they are wound-inducible and often lead to a systemic response (Koiwa et al, 1997). Trypsin inhibitors have been detected in leaves, lateral buds, and seed tissue and a bi-functional alpha-amylase-trypsin inhibitor (Figure 2c) in stem, stem bark, apical meristem and leaf roll which are preferential targets for the sugarcane borer Diatraea saccharalis.…”

Section: Proteinase Inhibitorsmentioning

confidence: 99%

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Mechanisms of sugarcane response to herbivory

et al. 2001

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Deciphering plant-insect interactions at the molecular level is one of the major topics of interest in contemporary plant biology research. In the last few years, various aspects of the plant response to insect damage have been investigated, including the characterization of direct and indirect responses, the regulation of gene expression resulting from insect attack and the signal transduction pathways. Such research has resulted in the proposal of new methods to enhance host resistance to insect pests, including the use of insecticidal genes that can be transferred by genetic engineering into target crops. By integrating the understanding of how plants react to insect damage with the techniques of molecular biology researchers should be able to increase the wide range of methods available for the control of insect pests. The sugarcane transcriptome project (SUCEST) has allowed the identification of several orthologues genes involved in the plant response to insect damage. In this paper we summarize several aspects of the complex interaction between plants and insects and describe the use of in silico analysis to provide information about gene expression in different sugarcane tissues in response to insect attack.

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Section: Systemic Signals Induced By Herbivory and Woundingmentioning

confidence: 98%

Section: Proteinase Inhibitorsmentioning

confidence: 99%

Mechanisms of sugarcane response to herbivory

et al. 2001

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“…Protease inhibitors play important role in plant defense mechanism by preventing proteolysis in the midgut of insect larvae leading to their starvation and subsequent death (Gatehouse et al, 1999). This fact can be interpreted as a potential strategy for increasing the level of plant defense against insects (Koiwa et al, 1997). Many reports have demonstrated retardation in the growth and development of insect pests fed on diets incorporating PIs, or on transgenic plants expressing PIs (deLeo and Gallerani, 2002;Murdock and Shade, 2002;Telang et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of trypsin inhibitor from Cicer arietinum L. and its efficacy against Helicoverpa armigera

Kansal

Kumar

Kuhar

et al. 2008

Braz. J. Plant Physiol.

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Protease inhibitors in legumes are one of the most promising weapons that confer resistance against insects by inhibiting proteases present in the gut of insect larvae. In the present study, trypsin inhibitor activity was detected in the seed flour extracts of 10 selected varieties of chickpea. The presence of inhibitor was confirmed by dot blot analysis. All the varieties showed inhibitory activity in vitro against the gut protease of Helicoverpa armigera (HGP). Trypsin inhibitor has been purified to near homogeneity to 60.46 fold and 29.20% recovery from chickpea seeds using heat denaturation, ammonium sulphate fractionation, DEAE-Sephadex A-25 and Sephadex G-75. The purified inhibitor showed a single band on SDS-PAGE corresponding to molecular mass of 30,000 Da. The purified inhibitor was active over a wide pH range whereas it retained maximum activity between pH 6 and 10. The inhibitor protein was stable up to 80°C but retained only 40% of activity when heated at 100°C for 20 min. The inhibitor lost its complete activity at 121°C. The chickpea trypsin inhibitor exhibited inhibitory activity against Helicoverpa armigera both in vitro and in vivo. In insect bioassay, a progressive decline in larval weight, growth and survival as well as temporal extension of larval growth was observed after feeding H. armigera larvae on diet supplemented with increasing concentrations of chickpea trypsin inhibitor. The adult emergence was also adversely affected by the inhibitor. It may be concluded that chickpea trypsin inhibitor has insecticidal potential against H. armigera.

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“…Plant proteinase inhibitors, a-amylase inhibitors, lectins and chitin binding proteins are usually expressed in response to herbivore insects, pathogens and wounding (Ryan, 1990;Botella et al, 1996;Koiwa et al, 1997;Sales et al, 2001) and may affect development by interfering in the proteolytic processes of a wide range of potentially damaging organisms, including bacteria, fungi, nematodes (Haq et al, 2004) and insects (Terra and Ferreira, 1994;Pompermayer, et al, 2001;Falco and Silva-Filho, 2003). The effects on insects can be observed when digestive enzymes are inhibited or when defense proteins bind to the gut structures of larvae and insects (XavierFilho, 1993).…”

Section: Introductionmentioning

confidence: 99%

Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets

Silva

Alcazar

Macedo

et al. 2006

Insect Biochemistry and Molecular Biology

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The digestive system of Ceratitis capitata was characterized during its larval development and in the insect stage. Disaccharidases against maltose and sucrose were more evident in the 2nd and 3rd day of larval development and in the adult stage, respectively. Glycosil-hydrolyses with higher specific a-galactosidasic and b-galactosidasic activities were detected in the 2nd and 3rd day of the larval stage, respectively. Specific proteolytic activities against azocasein showed an increase in the 4th and 5th day of the larval stage and in the adult stage. Specific hemoglobin activities were constant between 2nd and 6th day of the larval stage. The larvae used mainly serine proteinases, such as trypsin/chymotrypsin, and the adult insects only chymotrypsin-like enzymes in their digestive process. Two serine proteinases were separated from zymogram between the 4th and 5th day of larval development and in the adult stage. Effect of soybean trypsin inhibitor (SBTI, a serine proteinase inhibitor) on development of C. capitata was examined by bioassay. C. capitata was susceptible to SBTI which affected larval mass at ED 50 3.01%. r

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Regulation of protease inhibitors and plant defense

Cited by 425 publications

References 31 publications

Mechanisms of sugarcane response to herbivory

Mechanisms of sugarcane response to herbivory

Purification and characterization of trypsin inhibitor from Cicer arietinum L. and its efficacy against Helicoverpa armigera

Digestive enzymes during development of Ceratitis capitata (Diptera:Tephritidae) and effects of SBTI on its digestive serine proteinase targets

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