Regulation of Protein Function and Signaling by Reversible Cysteine S-Nitrosylation

Gould, Neal S.; Doulias, Paschalis‐Thomas; Τενοπούλου, Μαργαρίτα; Raju, Karthik; Ischiropoulos, Harry

doi:10.1074/jbc.r113.460261

Cited by 264 publications

(192 citation statements)

References 67 publications

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“…This nomenclature could also accommodate chemical species not currently identified in vivo or in vitro, such as reaction products of cysteine sulfur with nitrogen oxide (for review of cysteine S-nitrosylation, see [8]). Figure 1 indicates the domain structure of HMGB1 and its family members; we expect HMGB2 to undergo the same posttranslational modification as HMGB1, although so far these modifications have not been identified or even been sought after.…”

mentioning

confidence: 99%

A Systematic Nomenclature for the Redox States of High Mobility Group Box (HMGB) Proteins

Antoine

Harris

Andersson

et al. 2014

Mol Med

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mentioning

confidence: 99%

A Systematic Nomenclature for the Redox States of High Mobility Group Box (HMGB) Proteins

Antoine

Harris

Andersson

et al. 2014

Mol Med

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“…Although the physiological relevance of S-nitrosylation has been shown in several proteins [79], there has been an active debate on its role in the nervous system among NO researchers [50]. One possible function of S-nitrosylation may be neuroprotection via the elimination of excess NO which would otherwise promote oxidative stress [80].…”

Section: Downstream Targets Of No In the Pcmentioning

confidence: 99%

Nitric oxide-coupled signaling in odor elicited molecular events in the olfactory center of the terrestrial snail, Helix pomatia

Serfözö

Nacsa

Veréb

et al. 2017

Cellular Signalling

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Olfaction, a chemosensory modality, plays a pivotal role in the orientation and behavior of invertebrates. The central olfactory processing unit in terrestrial stylomatophoran snails is the procerebrum, which contains NO synthesizing interneurons, whose oscillatory currents are believed to be the base of odor evoked memory formation. Nevertheless, in this model the up-and downstream events of molecular cascades that trigger and follow NO release, respectively, have not been studied. Immunocytochemistry and flow cytometry studies performed on procerebral neural perikarya isolated from the snail Helix pomatia revealed cell populations with discrete DAF-2 fluorescence, indicating the release of different amounts of NO. Glutamate increased the intensity of DAF-2 fluorescence, and the number of DAF-2 positive non-bursting interneurons, through a mechanism likely to involve an NMDA-like receptor. Similarly to glutamate, NO activation induced an increase in intracellular cGMP levels through activation of soluble guanylyl cyclase. Immunohistochemical localization of proteins possessing the phosphorylated target sequence of AGC family kinases (RXXS/T-P), among them protein kinase A (RRXS/T-P), showed striking similarities to the distribution of NOS/cGMP. Activators of cyclic nucleotide synthesis increased the AGC-kinase-dependent phosphorylation of discrete proteins with 28, 45, and 55 kDa mw. Importantly, exposure of snails to an attractive odorant induced hyperphosphorylation of the 28 kDa protein, and increased levels of cGMP synthesis. Protein S-nitrosylation and intercellular activation of protein kinase G were also suggested as Abbreviations: 5-HT, 5-hydroxytryptamine or serotonin; ACh, acetylcholine; AGC-S-P, phosphorylated substrate of the AGC-family protein kinase; Akt, protein kinase B; AMPA, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid; APV, (2R)-amino-5-phosphonovaleric acid; B cell, large bursting neuron in the procerebrum; β-NADP, β-nicotinamide adenine dinucleotide; β-NADPH, β-nicotinamide adenine dinucleotide 2′-phosphate; BSA, bovine serum albumin; cAMP, 3′,5′-cyclic adenosine monophosphate;

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“…One putative S-nitrosylation site has been identified. S-nitrosylation reactions signal a broad spectrum of cellular activities including transcriptional and posttranscriptional regulation of protein expression as well as regulation of membrane, cytosolic, mitochondrial, nuclear, and extracellular protein functions (Gould et al, 2013). All these PTMs suggest various cellular functions and processes including cell cycle regulation; DNA repair; chromosomal maintenance; modification of cytoplasmic signal transduction, nuclear import, and subnuclear compartmentalization; DNA repair; transcription regulation; and stress response.…”

Section: Posttranslational Modificationsmentioning

confidence: 99%

Identification and characterization of a seed-specific grapevine dehydrin involved in abiotic stress response within tolerant varieties

Hanana¹,

Daldoul²,

Fouquet³

et al. 2014

Turk J Bot

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To identify and isolate genes related to abiotic stress (salinity and drought) tolerance in grapevine, a candidate gene approach led to the isolation from Cabernet Sauvignon cultivar of a full-length cDNA of dehydrin gene. The latter, named VvDhn, which is highly and mainly induced in late embryogenesis in seeds, encodes for a protein of 124 amino acids with a predicted molecular mass of 13.3 kDa. Details of the physicochemical parameters and structural properties (molecular mass, secondary structure, conserved domains and motives, and putative posttranslational modification sites) of the encoded protein have also been elucidated. The expression study of VvDhn was carried out within plant organs and tissues as well as under drought and salt stresses. VvDhn was not detected in vegetative tissue, whereas it was only expressed during seed development (during late embryogenesis) at extremely high levels and was induced by salt and drought stresses as well as ABA application. Moreover, salt stress induced VvDhn expression in the tolerant variety (Razegui) but not the sensitive variety (Syrah), which did not display expression variation during stress; VvDhn expression level and salt-stress response depend on regulatory mechanisms that are efficient only in the tolerant variety. On the other hand, under drought stress VvDhn was induced in both tolerant and sensitive varieties, with higher levels in the tolerant variety. In addition, stress signal molecules such as ABA (applied alone or in combination with saccharose) induced VvDhn expression, even at low levels. Minimal knowledge about the role and functionality of this gene is necessary and constitutes a prerequisite for including VvDhn in grapevine abiotic stress tolerance improvement programs.

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Regulation of Protein Function and Signaling by Reversible Cysteine S-Nitrosylation

Cited by 264 publications

References 67 publications

A Systematic Nomenclature for the Redox States of High Mobility Group Box (HMGB) Proteins

A Systematic Nomenclature for the Redox States of High Mobility Group Box (HMGB) Proteins

Nitric oxide-coupled signaling in odor elicited molecular events in the olfactory center of the terrestrial snail, Helix pomatia

Identification and characterization of a seed-specific grapevine dehydrin involved in abiotic stress response within tolerant varieties

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