Regulation of Protein Synthesis in Virus-Infected Animal Cells

Kozak, Marilyn

doi:10.1016/s0065-3527(08)60265-1

Cited by 178 publications

(91 citation statements)

References 379 publications

(325 reference statements)

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“…The ORF begins with the second AUG codon from the terminus of the RNA; the first AUG has a UGA stop codon just 10 nt downstream. The ORF AUG has the preferred consensus sequence AUGG, and an A at the -3 position (Kozak, 1986), but a second AUG codon occurs within the ORF only 36 nt downstream from the first.…”

Section: Cdna Cloning and Sequence Analysismentioning

confidence: 99%

Maize stripe virus RNA5 is of negative polarity and encodes a highly basic protein

Huiet

Tsai²,

Falk³

1993

Journal of General Virology

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The entire 1317 nucleotide sequence of the maize stripe virus (MStV) RNA5 was determined. Only one open reading frame (ORF) was identified and was found in the viral complementary RNA (vcRNA). This ORF appears to encode a protein ofM r 44237, hereafter referred to as NS 5. In vitro translation of transcripts representing nearly full-length RNA5 vcRNA yielded products of the predicted size, as well as some smaller, less prominent products. No products were identified from transcripts of viral polarity. RNA hybridization analyses of MStVinfected Zea mays revealed RNAs corresponding only to full-length RNA5, but both positive and negative polarity RNAs were abundant. Analysis of the NS 5 amino acid sequence revealed that it is extremely basic, containing 21% arginine and lysine. Database comparisons showed that NS 5 had no significant similarity to other protein sequences.

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Section: Cdna Cloning and Sequence Analysismentioning

confidence: 99%

Maize stripe virus RNA5 is of negative polarity and encodes a highly basic protein

Huiet

Tsai²,

Falk³

1993

Journal of General Virology

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“…The scanning complex, comprising the 40S ribosomal subunit and initiation factors (1,2), binds to the 5Ј end of mRNA and migrates downstream until the complex reaches an AUG codon embedded in an optimum context for the initiation of protein synthesis (3). For the majority of eukaryotic mRNAs, the first AUG encountered by the scanning complex serves as the translation initiation codon (first-AUG rule).…”

mentioning

confidence: 99%

An unusual internal ribosomal entry site of inverted symmetry directs expression of a potato leafroll polerovirus replication-associated protein

Jaag

Kawchuk

Rohde

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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Potato leafroll polerovirus (PLRV) genomic RNA acts as a polycistronic mRNA for the production of proteins P0, P1, and P2 translated from the 5 -proximal half of the genome. Within the P1 coding region we identified a 5-kDa replication-associated protein 1 (Rap1) essential for viral multiplication. An internal ribosome entry site (IRES) with unusual structure and location was identified that regulates Rap1 translation. Core structural elements for internal ribosome entry include a conserved AUG codon and a downstream GGAGAGAGAGG motif with inverted symmetry. Reporter gene expression in potato protoplasts confirmed the internal ribosome entry function. Unlike known IRES motifs, the PLRV IRES is located completely within the coding region of Rap1 at the center of the PLRV genome.

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“…It has been reported that host cell protein synthesis was rapidly or selectively inhibited by virus infection (Kozak, 1986;Schneider & Shenk, 1987;Sonenberg, 1987). This phenomenon has been shown in cells infected by some iridoviruses such as Chilo iridescent virus (Cerutti & Devauchelle, 1980) and Frog virus 3 (Chinchar & Yu, 1992).…”

Section: Viral Infection Causes Differential Expression Of Host Proteinsmentioning

confidence: 88%

iTRAQ analysis of Singapore grouper iridovirus infection in a grouper embryonic cell line

Chen

Tran

Lin

et al. 2008

Journal of General Virology

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We report, here, the first proteomics study of a grouper embryonic cell line (GEC) infected by Singapore grouper iridovirus (SGIV). The differential proteomes of GEC with and without viral infection were studied and quantified with iTRAQ labelling followed by liquid chromatography/ tandem mass spectrometry (LC-MS/MS). Forty-nine viral proteins were recognized, of which 11 were identified for the first time. Moreover, 743 host proteins were revealed and classified into 218 unique protein groups. Fourteen host proteins were upregulated and five host proteins were downregulated upon viral infection. The iTRAQ analysis of SGIV infection in GEC provides an insight to viral and host gene products at the protein level. This should facilitate further study and the understanding of virus-host interactions, molecular mechanisms of viral infection and pathogenesis.

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Regulation of Protein Synthesis in Virus-Infected Animal Cells

Cited by 178 publications

References 379 publications

Maize stripe virus RNA5 is of negative polarity and encodes a highly basic protein

Maize stripe virus RNA5 is of negative polarity and encodes a highly basic protein

An unusual internal ribosomal entry site of inverted symmetry directs expression of a potato leafroll polerovirus replication-associated protein

iTRAQ analysis of Singapore grouper iridovirus infection in a grouper embryonic cell line

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