Regulation of root secondary metabolites by partial root‐associated microbiotas under the shaping of licorice ecotypic differentiation in northwest China

Liu, Yang; Wang, Hao; Peng, Ziyi; Da, Li; Chen, Weimin; Jiao, Shuo

doi:10.1111/jipb.13179

Cited by 15 publications

(4 citation statements)

References 95 publications

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“…The hypervariable V4-V5 region of the 16S rRNA gene was selected for the amplification of bacterial sequences using the 515F (GTG CCA GCMGCC GCG GTAA)/907R (CCG TCA ATT CCT TTG AGT TT) primer pair [50]. All polymerase chain reactions (PCR) were performed based on the procedures described in a previous study [67]. Triplicate PCR amplicons were pooled together and then mixed with a similar volume of 1 × loading buffer (containing SYB green).…”

Section: Amplicon Sequencing and Bioinformatics Analysesmentioning

confidence: 99%

Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy

et al. 2023

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Background Allelopathy is closely associated with rhizosphere biological processes, and rhizosphere microbial communities are essential for plant development. However, our understanding of rhizobacterial communities under influence of allelochemicals in licorice remains limited. In the present study, the responses and effects of rhizobacterial communities on licorice allelopathy were investigated using a combination of multi-omics sequencing and pot experiments, under allelochemical addition and rhizobacterial inoculation treatments. Results Here, we demonstrated that exogenous glycyrrhizin inhibits licorice development, and reshapes and enriches specific rhizobacteria and corresponding functions related to glycyrrhizin degradation. Moreover, the Novosphingobium genus accounted for a relatively high proportion of the enriched taxa and appeared in metagenomic assembly genomes. We further characterized the different capacities of single and synthetic inoculants to degrade glycyrrhizin and elucidated their distinct potency for alleviating licorice allelopathy. Notably, the single replenished N (Novosphingobium resinovorum) inoculant had the greatest allelopathy alleviation effects in licorice seedlings. Conclusions Altogether, the findings highlight that exogenous glycyrrhizin simulates the allelopathic autotoxicity effects of licorice, and indigenous single rhizobacteria had greater effects than synthetic inoculants in protecting licorice growth from allelopathy. The results of the present study enhance our understanding of rhizobacterial community dynamics during licorice allelopathy, with potential implications for resolving continuous cropping obstacle in medicinal plant agriculture using rhizobacterial biofertilizers.

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Section: Amplicon Sequencing and Bioinformatics Analysesmentioning

confidence: 99%

Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy

et al. 2023

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“…Interestingly, the endophytic microbiota compositions were also significantly influenced by the environmental factors, as well as the content of total flavonoids in licorice roots (Dang et al 2020 ). Therefore, the flavonoid biosynthesis and accumulation in G. uralensis is inferred to be driven by the environment-endophyte-plant interactions (Liu et al 2021 ), but the detailed mechanisms are undeclared yet.…”

Section: Introductionmentioning

confidence: 99%

Abiotic factors and endophytes co-regulate flavone and terpenoid glycoside metabolism in Glycyrrhiza uralensis

Liu

et al. 2023

Appl Microbiol Biotechnol

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Recently, endorhizospheric microbiota is realized to be able to promote the secondary metabolism in medicinal plants, but the detailed metabolic regulation metabolisms and whether the promotion is influenced by environmental factors are unclear yet. Here, the major flavonoids and endophytic bacterial communities in various Glycyrrhiza uralensis Fisch. roots collected from seven distinct places in northwest China, as well as the edaphic conditions, were characterized and analyzed. It was found that the soil moisture and temperature might modulate the secondary metabolism in G. uralensis roots partially through some endophytes. One rationally isolated endophyte Rhizobium rhizolycopersici GUH21 was proved to promote the accumulation of isoliquiritin and glycyrrhizic acid significantly in roots of the potted G. uralensis under the relatively high-level watering and low temperature. Furthermore, we did the comparative transcriptome analysis of G. uralensis seedling roots in different treatments to investigate the detailed mechanisms of the environment-endophyte-plant interactions and found that the low temperature went hand in hand with the high-level watering to activate the aglycone biosynthesis in G. uralensis, while GUH21 and the high-level watering cooperatively promoted the in planta glucosyl unit production. Our study is of significance for the development of methods to rationally promote the medicinal plant quality. Key points • Soil temperature and moisture related to isoliquiritin contents in Glycyrrhiza uralensis Fisch. • Soil temperature and moisture related to the hosts’ endophytic bacterial community structures. • The causal relation among abiotic factors—endophytes—host was proved through the pot experiment.

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“…It is well known that there are quality differences of medicines from different distribution origins ( Liu et al., 2019b ). Previous studies have predominantly examined the impact of external factors, including soil conditions and climate factors, on the formation of herb quality ( Liu et al., 2021 ; Li et al., 2022 ; Liu et al., 2022 ). However, the influence of the internal environment of medicinal plants on the formation of herbal quality is receiving increasing attention.…”

Section: Introductionmentioning

confidence: 99%

Geographically associated endophytic fungi contribute to the tropane alkaloids accumulation of Anisodus tanguticus

Wang,

Chen,

Xiao

et al. 2023

Front. Plant Sci.

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Anisodus tanguticus is a valuable plant for extracting tropane alkaloids. However, the mechanisms by which plant microbiome mediate the accumulation of tropane alkaloids in Anisodus tanguticus are still not well understood. In this study, we collected 55 wild Anisodus tanguticus populations on the Tibetan Plateau and the tropane alkaloids content, and root-related bacteria and fungi diversity were analyzed using HPLC and 16 s rDNA and ITS sequencing. The results showed that tropane alkaloids content has obvious geographical distribution characteristics. Anisodine content had a significant positive correlation with latitude, while anisodamine and atropine content had a significant negative correlation with latitude. Variation partition analysis (VPA) showed that root endophytes play a significant role in promoting tropane alkaloid production in Anisodus tanguticus roots. The root endophytes alone explained 14% of the variation, which was the largest contributor. Soil properties variables could independently explain 5% of the variation, and climate variables could explain 1% of the variation. Of these, endophytic fungi alone accounted for 11%, while bacteria explained only 5%. Random forests and Mantel test showed that different regionally enriched endophytic fungi have a greater impact on the accumulation of tropane alkaloids than the whole endophytic fungi. Richness and relative abundance of enriched endophytic fungi in Hengduan-Qilian Mountains (HQ) group has a significant positive correlation with anisodine content, while richness and relative abundance of enriched endophytic fungi in Himalayas-Hengduan Mountains (HH) group has a significant positive correlation with anisodamine and atropine content. And, these enriched endophytic fungi have high network connectivity and distributed in separate network modules. This study further confirmed that endophytes were closely related to tropane alkaloids accumulation in Anisodus tanguticus and contribute to promote sustainable development, cultivation, and precision medicine of Anisodus tanguticus.

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Regulation of root secondary metabolites by partial root‐associated microbiotas under the shaping of licorice ecotypic differentiation in northwest China

Cited by 15 publications

References 95 publications

Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy

Metagenomics insights into responses of rhizobacteria and their alleviation role in licorice allelopathy

Abiotic factors and endophytes co-regulate flavone and terpenoid glycoside metabolism in Glycyrrhiza uralensis

Geographically associated endophytic fungi contribute to the tropane alkaloids accumulation of Anisodus tanguticus

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