Regulation of Starch Biosynthesis

Ball, Steven

doi:10.1007/0-306-48204-5_29

Cited by 21 publications

(21 citation statements)

References 61 publications

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“…The in vitro phenotype of the sta7-10 mutant is therefore the inability to degrade glycogen; however, the in vivo phenotype is attenuated starch formation. These results are consistent with the biochemical characterization of previously isolated C. reinhardtii STA7 mutants Ball, 1998;Myers et al, 2000).…”

Section: Starch Characterizationsupporting

confidence: 92%

“…The characterization of one of these mutants, sta7-10, revealed the disruption of a gene with high homology to the isoamylase gene family, a class of enzymes found in a variety of photosynthetic organisms. This enzyme is critical for the formation of insoluble starch in C. reinhardtii Mouille et al, 1996;Ball, 1998;Myers et al, 2000;Dauvillee et al, 2001b). The mutant reported here stores <3% of the insoluble starch compared with the wild-type strain.…”

Section: Introductionmentioning

confidence: 75%

“…The STA7 enzyme is involved in starch metabolism, and, in C. reinhardtii, it is necessary for the accumulation of insoluble starch Mouille et al, 1996;Ball, 1998;Myers et al, 2000;Dauvillee et al, 2001b). To verify that the sta7-10 mutant was compromised in the accumulation of starch, glucose levels were assayed from the insoluble starch pellet (see Methods) and shown to be <3% of those found in the CC425 background strain (data not shown).…”

Section: Starch Characterizationmentioning

confidence: 99%

“…Several additional C. reinhardtii mutants with variable starch content have been characterized previously (Ball, 1998), and these include the sta1, sta6, sta8, and sta7 strains Van den Koornhuyse et al, 1996;Dauvillee et al, 2001a;Zabawinski et al, 2001). These starch mutants were generously provided to us by Steven Ball and were used to determine whether other low-starch mutants have attenuated H 2 photoproduction rates.…”

Section: Additional Starch Mutantsmentioning

confidence: 99%

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Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii

et al. 2004

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DNA insertional transformants of Chlamydomonas reinhardtii were screened chemochromically for attenuated H 2 production. One mutant, displaying low H 2 gas photoproduction, has a nonfunctional copy of a gene that shows high homology to the family of isoamylase genes found in several photosynthetic organisms. DNA gel blotting and gene complementation were used to link this isoamylase gene to previously characterized nontagged sta7 mutants. This mutant is therefore denoted sta7-10. In C. reinhardtii, the STA7 isoamylase gene is important for the accumulation of crystalline starch, and the sta7-10 mutant reported here contains <3% of the glucose found in insoluble starch when compared with wild-type control cells. Hydrogen photoproduction rates, induced after several hours of dark, anaerobic treatment, are attenuated in sta7 mutants. RNA gel blot analysis indicates that the mRNA transcripts for both the HydA1 and HydA2 [Fe]-hydrogenase genes are expressed in the sta7-10 mutant at greater than wild-type levels 0.5 h after anaerobic induction. However, after 1.5 h, transcript levels of both HydA1 and HydA2 begin to decline rapidly and reach nearly undetectable levels after 7 h. In wildtype cells, the hydrogenase transcripts accumulate more slowly, reach a plateau after 4 h of anaerobic treatment, and maintain the same level of expression for >7 h under anaerobic incubation. Complementation of mutant cells with genomic DNA corresponding to the STA7 gene restores both the starch accumulation and H 2 production phenotypes. The results indicate that STA7 and starch metabolism play an important role in C. reinhardtii H 2 photoproduction. Moreover, the results indicate that mere anaerobiosis is not sufficient to maintain hydrogenase gene expression without the underlying physiology, an important aspect of which is starch metabolism.

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Section: Starch Characterizationsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 75%

Section: Starch Characterizationmentioning

confidence: 99%

Section: Additional Starch Mutantsmentioning

confidence: 99%

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Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii

et al. 2004

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“…AGPase can be activated by 3-phosphoglyceric acid (3-PGA) and inhibited by orthophosphate (Pi), and the activity is thus regulated by the 3-PGA/Pi ratio (Ball 1998). Li et al (2011) reported a 39.2 and 45 % decrease of AGPase activity and starch synthesis, respectively, in the microalga Pseudochlorococcum sp.…”

Section: Discussionmentioning

confidence: 99%

Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions

Yao

Cao

et al. 2013

Appl Microbiol Biotechnol

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Microalgal starch is a potential feedstock for biofuel production. Nutrient stress is widely used to stimulate starch accumulation in microalgae. Cell growth and starch accumulation in the marine green microalga Tetraselmis subcordiformis were evaluated under extracellular phosphorus deprivation with initial cell densities (ICD) of 1.5, 3.0, 6.0, and 9.0×10 6 cells mL were all obtained in the culture with the ICD of 3.0× 10 6 cells mL −1 . Appropriate ICD could be used to regulate the intracellular phosphorus concentration and maintain adequate photosynthetic activity to achieve the highest starch productivity, along with biomass and starch concentration. The recovery of phosphorus-deprived T. subcordiformis in medium containing 0.5, 1.0, or 6.0 mM KH 2 PO 4 was also tested. Cell growth and starch accumulation ability could be recovered completely. A phosphorus pool in T. subcordiformis was shown to manipulate its metabolic activity under different environmental phosphorus availability. Though lower starch productivity and starch content were achieved under phosphorus deprivation compared with nitrogen-or sulfur-deprived conditions, the higher biomass and starch concentration make T. subcordiformis a good candidate for biomass and starch production under extracellular phosphorus deprivation.

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Kinetic modeling of light limitation and sulfur deprivation effects in the induction of hydrogen production with Chlamydomonas reinhardtii: Part I. Model development and parameter identification

Fouchard

Pruvost

Degrenne

et al. 2008

Biotech & Bioengineering

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Chlamydomonas reinhardtii is a green microalga capable of turning its metabolism towards H2 production under specific conditions. However this H2 production, narrowly linked to the photosynthetic process, results from complex metabolic reactions highly dependent on the environmental conditions of the cells. A kinetic model has been developed to relate culture evolution from standard photosynthetic growth to H2 producing cells. It represents transition in sulfur-deprived conditions, known to lead to H2 production in Chlamydomonas reinhardtii, and the two main processes then induced which are an over-accumulation of intracellular starch and a progressive reduction of PSII activity for anoxia achievement. Because these phenomena are directly linked to the photosynthetic growth, two kinetic models were associated, the first (one) introducing light dependency (Haldane type model associated to a radiative light transfer model), the second (one) making growth a function of available sulfur amount under extracellular and intracellular forms (Droop formulation). The model parameters identification was realized from experimental data obtained with especially designed experiments and a sensitivity analysis of the model to its parameters was also conducted. Model behavior was finally studied showing interdependency between light transfer conditions, photosynthetic growth, sulfate uptake, photosynthetic activity and O2 release, during transition from oxygenic growth to anoxic H2 production conditions.

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Regulation of Starch Biosynthesis

Cited by 21 publications

References 61 publications

Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii

Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii

Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions

Kinetic modeling of light limitation and sulfur deprivation effects in the induction of hydrogen production with Chlamydomonas reinhardtii: Part I. Model development and parameter identification

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