Regulation of Stearoyl-CoA Desaturase 1 mRNA Stability by Polyunsaturated Fatty Acids in 3T3-L1 Adipocytes

Sessler, Anna M.; Kaur, Navjot; Palta, Jiwan P.; Ntambi, James M.

doi:10.1074/jbc.271.47.29854

Cited by 138 publications

(114 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One possible explanation can be found in the increase in PUFA content in liver as BT was reduced in feeds. It has been reported that PUFA inhibit the activity of stearoyl-CoA-desaturase, the enzyme that synthesizes oleic acid from stearic acid, thus regulating its expression (Sessler et al, 1996). This inhibition could lead to accumulation of the precursor (C18:0), and reduction in MUFA content, which might also be affected by the lower MUFA supply in the diet as vegetable fat increased in feeds.…”

Section: Resultsmentioning

confidence: 99%

Dietary n-6- or n-3-rich vegetable fats and α-tocopheryl acetate: effects on fatty acid composition and stability of rabbit plasma, liver and meat

Tres

Bou

Codony

et al. 2009

Animal

View full text Add to dashboard Cite

We supplemented diets with a-tocopheryl acetate (100 mg/kg) and replaced beef tallow (BT) in feeds with increasing doses of n-6-or n-3-rich vegetable fat sources (linseed and sunflower oil), and studied the effects on the fatty acid (FA) composition, the a-tocopherol (aT) content and the oxidative stability of rabbit plasma and liver. These effects were compared with those observed in a previous study in rabbit meat. As in meat, the content of saturated, monounsaturated and trans FA in plasma and liver mainly reflected feed FA profile, except stearic acid in liver, which increased as feeds contained higher doses of vegetable fat, which could be related to an inhibition of the activity of the stearoyl-CoA-desaturase. As linseed oil increased in feeds, the n-6/n-3 FA ratio was decreased in plasma and liver as a result of the incorporation of FA from diets and also, due to the different performance and selectivity of desaturase enzymes. However, an increase in the dose of vegetable fat in feeds led to a significant reduction in the aT content of plasma and liver, which was greater when the fat source was linseed oil. Increasing the dose of vegetable fat in feeds also led to an increase in the susceptibility to oxidation (lipid hydroperoxide (LHP) value) of rabbit plasma, liver and meat and on the thiobarbituric acid (TBA) values of meat. Although the dietary supplementation with a-tocopheryl acetate increased the aT content in plasma and liver, it did not modify significantly their TBA or LHP values. In meat however, both TBA and LHP values were reduced by the dietary supplementation with a-tocopheryl acetate. The plasma aT content reflected the aT content in tissues, and correlated negatively with tissue oxidability. From the studied diets, those containing 1.5% linseed oil plus 1.5% BT and 100 mg of a-tocopheryl acetate/kg most improved the FA composition and the oxidative stability of rabbit tissues.

show abstract

Section: Resultsmentioning

confidence: 99%

Dietary n-6- or n-3-rich vegetable fats and α-tocopheryl acetate: effects on fatty acid composition and stability of rabbit plasma, liver and meat

Tres

Bou

Codony

et al. 2009

Animal

View full text Add to dashboard Cite

show abstract

“…This discrepancy might be explained by the fact that PUFA contents of the experimental rations used by Archibeque et al (2005) were not constant thereby interfering with proper interpretation of the data. Indeed, PUFA are known to have a down-regulating effect on the activity of SCD (Sessler et al, 1996;Ntambi, 1999;Velliquette et al, 2009). In beef cattle, it was specifically shown that the SCD activity in muscle and adipose tissue was lower on a diet rich in n-3 PUFA compared with n-6 PUFA (Herdmann et al, 2010;Hiller et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, PUFA have a down-regulating effect on the activity of SCD (Ntambi, 1999). Both n-3 and n-6 PUFA may depress SCD activity as shown in vitro in hepatocytes (Velliquette et al, 2009) and adipocytes (Sessler et al, 1996). In beef cattle, SCD gene expression in muscle and SCD protein expression and enzyme activity in muscle and adipose tissue was significantly lower on a diet rich in n-3 PUFA compared with n-6 PUFA (Herdmann et al, 2010;Hiller et al, 2011).…”

Section: Introductionmentioning

confidence: 96%

Effect of whole cottonseed v. sunflower seed on the fatty acid profile of subcutaneous fat, longissimus dorsi and blood of Thai Native and Holstein bulls

Polviset

Schonewille

Everts

et al. 2015

Animal

View full text Add to dashboard Cite

In a 2 × 2 factorial design, 12 Thai Native and 12 Holstein bulls were fed ad libitum a total mixed ration (20 : 80; roughage : concentrate) with whole cottonseed (WCS) or sunflower seed (SFS) as oilseed sources. The rations contained 7% crude fat and were fed for 90 days. Plasma was taken at three times during the experiment, and at slaughter the longissimus dorsi and subcutaneous fat were sampled for fatty acid analysis. Ration did not affect rumen fermentation parameters. The plasma fatty acid profile was not affected by ration. In subcutaneous fat, a ration × breed interaction for the saturated fatty acid (SFA) and c9t11 CLA proportions was observed, resulting from larger differences between the rations in Thai Native compared with Holstein bulls. The WCS ration resulted in higher proportions of SFA and lower proportions of monounsaturated fatty acids and c9,t11 CLA compared with the SFS ration ( P < 0.01). In the intramuscular fat, the WCS ration was also associated with a lower c9t11 CLA proportion ( P < 0.01) and higher SFA proportion ( P < 0.05). The intramuscular proportion of polyunsaturated acids was higher and the proportion of SFA was lower in Thai Native compared with Holstein bulls ( P < 0.05), irrespective of ration.

show abstract

“…The mechanism of PUFA-mediated repression of lipogenic gene expression in adipocytes is different from that described in liver. PUFA also decrease SCD1 mRNA levels in 3T3-L1 adipocytes (Sessler et al 1996). PUFA suppress the expression of the SCD1 gene in adipocytes by decreasing mRNA stability.…”

Section: Effect Of Polyunsaturated Fatty Acids On Adipose Tissue Genementioning

confidence: 99%

“…In order to further address the molecular basis by which PUFA might influence adipose tissue gene expression, it has been shown that PUFA act directly on adipocyte gene expression (Grimaldi et al 1992;Amri et al 1994;Sessler et al 1996;Mater et al 1998). This finding indicates that PUFA-mediated regulation of adipose tissue gene expression would not necessarily need extra-adipose factors.…”

Section: Effect Of Polyunsaturated Fatty Acids On Adipose Tissue Genementioning

confidence: 99%

Selectivity of fatty acids on lipid metabolism and gene expression

Raclot¹,

Oudart²

1999

Proc. Nutr. Soc.

View full text Add to dashboard Cite

Triacylglycerols represent the main form of storage for a wide spectrum of fatty acids. Their utilization first involves mobilization from adipose tissue through lipolysis. The release of individual fatty acids from adipose tissue is selective in vitro and in vivo in animal studies and also in human subjects. Generally, fatty acids are more readily mobilized from fat cells when they are short-chain and unsaturated. This selectivity could affect the storage of individual fatty acids in adipose tissue, and their subsequent supply to tissues. The nature of the dietary fats could affect lipid homeostasis and body fat deposition. Dietary fish oil influences adipose tissue development in a site-specific manner as a function of diet and feeding period. A diet high in n-3 polyunsaturated fatty acids (PUFA) results in a preferential partitioning of ingested energy towards oxidation at the expense of storage. Fatty acids are important mediators of gene expression in the liver. Indeed, genes encoding both glycolytic and lipogenic enzymes and key metabolic enzymes involved in fatty acid oxidation are regulated by dietary PUFA. White adipose tissue could also be a target for PUFA control of gene expression. The treatment of pre-adipose cells by fatty acids induces the expression of numerous genes that encode proteins involved in fatty acid metabolism. The mechanisms of PUFA-mediated repression of gene expression in adipocytes seem to be different, at least partly, from those described in liver. Tissue-specific and site-specific factors are possibly involved in the specific effect of PUFA on gene expression, although other mechanisms cannot be excluded.

show abstract

Regulation of Stearoyl-CoA Desaturase 1 mRNA Stability by Polyunsaturated Fatty Acids in 3T3-L1 Adipocytes

Cited by 138 publications

References 44 publications

Dietary n-6- or n-3-rich vegetable fats and α-tocopheryl acetate: effects on fatty acid composition and stability of rabbit plasma, liver and meat

Dietary n-6- or n-3-rich vegetable fats and α-tocopheryl acetate: effects on fatty acid composition and stability of rabbit plasma, liver and meat

Effect of whole cottonseed v. sunflower seed on the fatty acid profile of subcutaneous fat, longissimus dorsi and blood of Thai Native and Holstein bulls

Selectivity of fatty acids on lipid metabolism and gene expression

Contact Info

Product

Resources

About