Regulation of substrate surface topography on differentiation of mesenchymal stem cells

Huo, Bo; Zhao, Yang; Bai, Xue; Sun, Qing; Jiao, Fei

doi:10.1007/s10409-020-00997-6

Cited by 4 publications

(1 citation statement)

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“…Application of topographies to improve in vitro cell culture conditions can be achieved by introducing features to the surface of cell culture flasks, plates, or carriers to generate high-quality homogeneous cell populations at pluripotent or specific differentiation stages. The beneficial effects of micro-and nanotopographies on stem cell maintenance and expansion, reprogramming, and differentiation are previously described in this review as well as reviews published by Chan et al, 270 Fang et al, 295 and Huo et al 296 Following the model of some regenerative medicine companies such as Regeneus, specific effects from both cells and secreted molecules could be used for regenerative medicine. Besides in vitro cultures, topographies can be also used in medical implants and tissue-engineered scaffolds to influence cell behavior and improve the healing, regrowth, or replacement of damaged tissues, as proven in a wide variety of experiments.…”

Section: Translating Micro-and Nanotopographiesmentioning

confidence: 99%

The Bumpy Road to Stem Cell Therapies: Rational Design of Surface Topographies to Dictate Stem Cell Mechanotransduction and Fate

Carthew

Taylor

Garcia-Cruz

et al. 2022

ACS Appl. Mater. Interfaces

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Cells sense and respond to a variety of physical cues from their surrounding microenvironment, and these are interpreted through mechanotransductive processes to inform their behavior. These mechanisms have particular relevance to stem cells, where control of stem cell proliferation, potency, and differentiation is key to their successful application in regenerative medicine. It is increasingly recognized that surface micro- and nanotopographies influence stem cell behavior and may represent a powerful tool with which to direct the morphology and fate of stem cells. Current progress toward this goal has been driven by combined advances in fabrication technologies and cell biology. Here, the capacity to generate precisely defined micro- and nanoscale topographies has facilitated the studies that provide knowledge of the mechanotransducive processes that govern the cellular response as well as knowledge of the specific features that can drive cells toward a defined differentiation outcome. However, the path forward is not fully defined, and the “bumpy road” that lays ahead must be crossed before the full potential of these approaches can be fully exploited. This review focuses on the challenges and opportunities in applying micro- and nanotopographies to dictate stem cell fate for regenerative medicine. Here, key techniques used to produce topographic features are reviewed, such as photolithography, block copolymer lithography, electron beam lithography, nanoimprint lithography, soft lithography, scanning probe lithography, colloidal lithography, electrospinning, and surface roughening, alongside their advantages and disadvantages. The biological impacts of surface topographies are then discussed, including the current understanding of the mechanotransductive mechanisms by which these cues are interpreted by the cells, as well as the specific effects of surface topographies on cell differentiation and fate. Finally, considerations in translating these technologies and their future prospects are evaluated.

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Section: Translating Micro-and Nanotopographiesmentioning

confidence: 99%