2003
DOI: 10.4049/jimmunol.171.5.2287
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Regulation of Sustained Actin Dynamics by the TCR and Costimulation as a Mechanism of Receptor Localization

Abstract: The localization of receptors, signaling intermediates, and cytoskeletal components at the T cell/APC interface is thought to be a major determinant of efficient T cell activation. However, important questions remain open. What are the dynamics of the T cell cytoskeleton as a potential mediator of such localization? How are they regulated by the TCR and costimulatory receptors? Do they actually mediate receptor localization? In this study, we have addressed these questions. Even under limiting T cell activatio… Show more

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Cited by 91 publications
(120 citation statements)
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“…In cells treated with colchicine to disrupt microtubules, STIM1 localized to blebs that protruded from the top of the cell instead of forming a normal cap (Supplemental Figure S4, A and B). Evaluating the effects of disrupting the actin cytoskeleton was problematic because complete disassembly inhibits TCR activation, Ca 2ϩ flux and blocks spreading of T-cells (Valitutti et al, 1995;Bunnell et al, 2001;Tskvitaria-Fuller et al, 2003). We used a low dose of latrunculin to disrupt the actin network as visualized by actin-GFP (data not shown).…”
Section: An Intact Cytoskeleton Is Required For Normal Cap Formationmentioning
confidence: 99%
“…In cells treated with colchicine to disrupt microtubules, STIM1 localized to blebs that protruded from the top of the cell instead of forming a normal cap (Supplemental Figure S4, A and B). Evaluating the effects of disrupting the actin cytoskeleton was problematic because complete disassembly inhibits TCR activation, Ca 2ϩ flux and blocks spreading of T-cells (Valitutti et al, 1995;Bunnell et al, 2001;Tskvitaria-Fuller et al, 2003). We used a low dose of latrunculin to disrupt the actin network as visualized by actin-GFP (data not shown).…”
Section: An Intact Cytoskeleton Is Required For Normal Cap Formationmentioning
confidence: 99%
“…Internal -chain accumulation was defined as a spherical area of increased fluorescence (Ն40% above cellular background) at the center of the interface that was removed at least 1 area diameter from the T cell-APC interface, which did not have any overlap with the APC and did not last longer than two frames (i.e., 40 s). Both tat SEC7 and tat SEC7mt were constructed, expressed in Escherichia coli, and purified in analogy to the previously described tat WASP VCA domain (17) using the published SEC7 inactivating mutation and domain boundaries (18). The tat dynein L chain 1 (DLC1) peptide was the same as published (19) with the exception of a disulfide bond linking the tat and the DLC1 moieties instead of a peptide bond.…”
Section: Imaging and Image Analysismentioning
confidence: 99%
“…For two-color three-dimensional imaging, an actin-mCherry (21) fusion protein was generated and retrovirally expressed as actin-GFP (17). During image acquisition, both red and green images were acquired at each z plane before moving to the next plane.…”
Section: Imaging and Image Analysismentioning
confidence: 99%
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