Regulation of T cell differentiation: in vitro induction of 20 alpha-hydroxysteroid dehydrogenase in splenic lymphocytes from athymic mice by a unique lymphokine.

Ihle, James N.; Pepersack, L; Rebar, L

doi:10.4049/jimmunol.126.6.2184

Cited by 233 publications

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“… 5 Of note, similar roles of many of these cytokines have been described in related immune-mediated inflammatory diseases. 6 However, despite having been described back in the 1980s 7 8 and reported to be involved in pathologies such as experimental inflammatory arthritis, 9 10 the role of IL-3 signalling via the IL-3 receptor (IL-3R) in the development and perpetuation of chronic intestinal inflammation remains largely unexplored.…”

Section: Introductionmentioning

confidence: 99%

IL-3 receptor signalling suppresses chronic intestinal inflammation by controlling mechanobiology and tissue egress of regulatory T cells

Ullrich,

Derdau,

Baltes

et al. 2023

Gut

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IL-3 has been reported to be involved in various inflammatory disorders, but its role in inflammatory bowel disease (IBD) has not been addressed so far. Here, we determined IL-3 expression in samples from patients with IBD and studied the impact ofIl3orIl3rdeficiency on T cell-dependent experimental colitis. We explored the mechanical, cytoskeletal and migratory properties ofIl3r−/−andIl3r+/+T cells using real-time deformability cytometry, atomic force microscopy, scanning electron microscopy, fluorescence recovery after photobleaching andin vitroandin vivocell trafficking assays. We observed that, in patients with IBD, the levels of IL-3 in the inflamed mucosa were increased.In vivo, experimental chronic colitis on T cell transfer was exacerbated in the absence of Il-3 or Il-3r signalling. This was attributable to Il-3r signalling-induced changes in kinase phosphorylation and actin cytoskeleton structure, resulting in increased mechanical deformability and enhanced egress of Tregs from the inflamed colon mucosa. Similarly, IL-3 controlled mechanobiology in human Tregs and was associated with increased mucosal Treg abundance in patients with IBD. Collectively, our data reveal that IL-3 signaling exerts an important regulatory role at the interface of biophysical and migratory T cell features in intestinal inflammation and suggest that this might be an interesting target for future intervention.

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Section: Introductionmentioning

confidence: 99%

IL-3 receptor signalling suppresses chronic intestinal inflammation by controlling mechanobiology and tissue egress of regulatory T cells

Ullrich,

Derdau,

Baltes

et al. 2023

Gut

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Expression of 20α‐hydroxysteroid dehydrogenase in nonlymphoid hemopoietic cell lines

1982

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Expression of 20 alpha-hydroxysteroid dehydrogenase (20 alpha-SDH), a putative T cell marker, has been examined in a number of nonlymphoid hemopoietic cell lines. Both promyelocytic and stromal bone marrow lines expressed significant 20 alpha-SDH activity which was not correlated with virus infection or growth rate. 20 alpha-SDH therefore is not a unique marker for T cells in hemopoietic tissues. This result is discussed in relationship to the action of the newly proposed interleukin 3.

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Interleukin-3-dependent expression of the c-myc and c-fos proto-oncogenes in hemopoietic cell lines.

Conscience¹,

Verrier²,

Martin³

1986

The EMBO Journal

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Using Northern blot analysis the expression of several proto‐oncogenes was studied in established lines of mast cell precursors. Upon removal of interleukin‐3 (IL‐3) from the culture medium, factor‐dependent cells stop dividing. During the first 7 h, however, normal amounts of total cellular mRNAs are maintained, and this is reflected in unchanged levels of several transcripts, such as actin, c‐Ha‐ras and c‐fes. In contrast, within 1.5 h of IL‐3 removal, the levels of c‐myc and c‐fos mRNAs decrease drastically and addition of IL‐3 at that stage quickly induces back the levels found in actively growing cultures. In factor‐independent cells, which proliferate actively even in the absence of IL‐3, high levels of c‐myc and c‐fos transcripts are maintained in the absence of growth factor. In cells arrested by serum starvation, addition of 10% serum induces massive amounts of c‐fos transcripts, but not of c‐myc, and cell proliferation is not restored. The data suggest that the c‐myc and c‐fos proto‐oncogenes play an important role in mediating the multiple effects of IL‐3 on hemopoietic progenitor cells.

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Regulation of T cell differentiation: in vitro induction of 20 alpha-hydroxysteroid dehydrogenase in splenic lymphocytes from athymic mice by a unique lymphokine.

Cited by 233 publications

References 0 publications

IL-3 receptor signalling suppresses chronic intestinal inflammation by controlling mechanobiology and tissue egress of regulatory T cells

IL-3 receptor signalling suppresses chronic intestinal inflammation by controlling mechanobiology and tissue egress of regulatory T cells

Expression of 20α‐hydroxysteroid dehydrogenase in nonlymphoid hemopoietic cell lines

Interleukin-3-dependent expression of the c-myc and c-fos proto-oncogenes in hemopoietic cell lines.

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