Regulation of telomerase by alternate splicing of human telomerase reverse transcriptase (hTERT) in normal and neoplastic ovary, endometrium and myometrium

The human telomerase gene: complete genomic sequence and analysis of tandem repeat polymorphisms in intronic regions

“…Thirty alleles of hTERT-VNTR 6-2nd were identi®ed. Data is presented as in Figure 2 ( Kilian et al, 1997;Armstrong et al, 2000;Ulaner et al, 2000).…”

Sp1 and Sp3 Recruit Histone Deacetylase to Repress Transcription of Human Telomerase Reverse Transcriptase (hTERT) Promoter in Normal Human Somatic Cells

“…The cDNA synthesis, reverse transcription, and quantitative PCR were performed essentially as previously described (25). The amplified DNA was separated on 1% agarose gel and stained with ethidium bromide.…”

Complex regulatory mechanisms of telomerase activity in normal and cancer cells: How can we apply them for cancer therapy?

“…Interestingly, splicing variants that lack the a site function as dominant-negative inhibitors of telomerase causing telomere shortening and eventually cell death (Colgin et al, 2000;Yi et al, 2000). In an analysis of clinical samples, tumors expressing splicing variants of hTERT have been shown to lack telomerase activity (Ulaner et al, 2000). However, some normal tissues with hTERT transcripts containing both a and b lack telomerase activity (Ulaner et al, 2000), suggesting that there are further mechanisms for suppressing telomerase activity downstream of hTERT transcription and mRNA splicing, and these mechanisms have been lost during neoplastic transformation.…”