Regulation of Th2 responses by Lactococcus lactis subsp. cremoris YRC3780 alleviates DNCB-induced atopic dermatitis in the mouse model

Wang, Rong; Nakajima‐Adachi, Haruyo; Wang, Yimei; Zhou, Yingyu; Gu, Wenting; Hiraide, Erika; Morinaga, Mamiko; Nakagawa, Ryogo; Nakamura, Shotaro; Takano, Toru; Li, Xuyang; Saeki, Mayumi; Kaminuma, Osamu; Hiroi, Takachika; Uchida, Kenji; Motoshima, Hidemasa; Tanokura, Masaru; Miyakawa, Takuya; Hachimura, Satoshi

doi:10.1016/j.jff.2022.105091

Cited by 4 publications

(4 citation statements)

References 43 publications

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“…In addition, although many studies have reported on the immunomodulatory functions of lactobacilli such as Lactobacillus ( 2 , 16 ), there are few reports on lactococci such as Lactococcus ( 2 , 6 , 10 ). Our results suggest that induction of Tregs and inhibition of Th2 cell differentiation may be the mechanisms underlying the alleviation of allergies by this Lactococcus strain ( 11 – 13 ). Our results are consistent with the down-regulation of Th2 response observed by this strain in murine allergy models ( 12 , 13 ).…”

Section: Discussionmentioning

confidence: 72%

“…Our results suggest that induction of Tregs and inhibition of Th2 cell differentiation may be the mechanisms underlying the alleviation of allergies by this Lactococcus strain ( 11 – 13 ). Our results are consistent with the down-regulation of Th2 response observed by this strain in murine allergy models ( 12 , 13 ). L. cremoris YRC3780 also enhanced the production of IL-10, an anti-inflammatory cytokine that is important for ameliorating inflammation, so this cytokine may also be involved in the antiallergic effect of L. cremoris YRC3780.…”

Section: Discussionmentioning

confidence: 72%

“…It has been shown that L. cremoris YRC3780 ameliorates the symptoms of pollinosis in both animal and human studies ( 11 , 12 ). In an atopic dermatitis-like murine model of skin inflammation, oral administration of L. cremoris YRC3780 has alleviated allergen-induced dermal responses ( 13 ). It was demonstrated in this previous study that L. cremoris YRC3780 enhanced IFN-γ and IL-12 production and inhibited IL-4 production by allergen-specific CD4 + T cells in the presence of various types of immune cells including intestinal DCs ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

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Lactococcus lactis subsp. cremoris YRC3780 modifies function of mesenteric lymph node dendritic cells to modulate the balance of T cell differentiation inducing regulatory T cells

Nakagawa,

Gu,

Mizobuchi

et al. 2024

Front. Immunol.

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IntroductionThe intestinal immune system plays a pivotal role in the induction of immune responses against food. In the case of T cell response, dendritic cells (DCs) are especially important. However, the regulation of immune responses to food by intestinal DCs has been poorly described. In this study, we analyzed the effect of Lactococcus lactis subsp. cremoris YRC3780, a lactic acid bacterial strain isolated from kefir, a traditional fermented milk product, on the immune responses induced by antigen presentation by intestinal DCs to T cells as well as the mechanism of action of these immunomodulatory effects. It has been shown that L. cremoris YRC3780 ameliorates the symptoms of pollinosis in both animal and human studies.MethodsCD11c+ cells from mesenteric lymph nodes (MLNs) of BALB/c mice were cultured as MLN DCs with L. cremoris YRC3780 and expression of genes inducing regulatory T cells (Tregs) was examined by qPCR. In addition, MLN DCs were cocultured with CD4+ T cells from DO11.10 transgenic mice expressing an ovalbumin (OVA)-specific TCR and the OVA antigen peptide and L. cremoris YRC3780. Induction of Tregs was examined by flow cytometry, gene expression was analyzed by DNA microarray and qPCR, and the production of cytokines was measured by ELISA. MLN DCs from TLR2-deficient mice and components of L. cremoris YRC3780 were used to examine the recognition of YRC3780 by MLN DCs.ResultsL. cremoris YRC3780 enhanced the expression of genes involved in Treg induction in MLN DCs and induced Foxp3+CD4+T cells in an MLN DC and CD4+ T-cell co-culture system. The effect on MLN DCs was likely mediated by receptors other than TLR2. Together with microarray analyses of CD4+ T cell gene expression and cytokine ELISA, it was demonstrated that L. cremoris YRC3780 promoted the induction of Th1 and Tregs, and regulated the balance of Th1/Th2 and Treg/Th17 cells involving multiple genes via the antigen-presentation of MLN DCs.DiscussionOur findings provide insights into the modulation of intestinal immune responses mediated by DCs and the antiallergic effects of lactic acid bacteria.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

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Lactococcus lactis subsp. cremoris YRC3780 modifies function of mesenteric lymph node dendritic cells to modulate the balance of T cell differentiation inducing regulatory T cells

Nakagawa,

Gu,

Mizobuchi

et al. 2024

Front. Immunol.

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“…Stratum corneum and tight junctions are barrier structures that prevent external penetration. Transmembrane proteins, such as claudin and occludin, and tight junction plaque proteins, such as zonula occludens (ZO), are the major components of tight junctions [ 5 ]. These tight junction proteins have also been associated with AD.…”

Section: Introductionmentioning

confidence: 99%

Use of a Silkworm (Bombyx mori) Larvae By-Product for the Treatment of Atopic Dermatitis: Inhibition of NF-κB Nuclear Translocation and MAPK Signaling

et al. 2023

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Atopic dermatitis (AD) is a long-lasting inflammatory skin disease that contributes to the global health burden and impacts 10–20% of the world’s population. In this study, we determined the anti-AD effect of a by-product of silkworm (Bombyx mori) larval powder, strain Yeonnokjam (SLPY), as a sustainable, natural source for the development of therapeutic agents for AD. HaCaT cells were used to assess the in vitro anti-inflammatory activity of SLPY, and a 1-chloro-2,4-dinitrobenzene (DNCB)-induced mouse model was used to study the in vivo anti-AD effects. SLPY treatment downregulated the expression of the inflammatory cytokines TNF-α, IL1β, IL-8, and Cox-2 in stimulated HaCaT cells. Similarly, the topical application of SLPY in DNCB-treated mice downregulated the expression of inflammatory cytokines and proteins while ameliorating the clinical features of AD. Further, SLPY treatment inhibited the nuclear translocation of NF-κb p65, thereby supporting the efficacy of SLPY in the treatment of AD.

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Editorial: Immunomodulation by food components via dendritic cells

Hachimura

2024

Front. Nutr.

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Regulation of Th2 responses by Lactococcus lactis subsp. cremoris YRC3780 alleviates DNCB-induced atopic dermatitis in the mouse model

Cited by 4 publications

References 43 publications

Lactococcus lactis subsp. cremoris YRC3780 modifies function of mesenteric lymph node dendritic cells to modulate the balance of T cell differentiation inducing regulatory T cells

Lactococcus lactis subsp. cremoris YRC3780 modifies function of mesenteric lymph node dendritic cells to modulate the balance of T cell differentiation inducing regulatory T cells

Use of a Silkworm (Bombyx mori) Larvae By-Product for the Treatment of Atopic Dermatitis: Inhibition of NF-κB Nuclear Translocation and MAPK Signaling

Editorial: Immunomodulation by food components via dendritic cells

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