Regulation of the ABA-responsive Em promoter by ABI3 in the moss Physcomitrella patens

Sakata, Yoichi; Nakamura, Izumi; Taji, Teruaki; Tanaka, Shigeo; Quatrano, Ralph S.

doi:10.4161/psb.5.9.11774

Cited by 42 publications

(31 citation statements)

References 38 publications

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“…These bZips may be involved in ACGT-core sequence-mediated activation of ABAinducible genes in the moss. Sakata et al (2010) conducted promoter analysis of the Em promoter responsible for ABI3 activation in P. patens and demonstrated that the RY sequence, to which the B3 domain of ABI3 binds directly, is essential for PpABI3 activation, whereas Arabidopsis ABI3 could effectively activate the Em promoter either in an ABRE-or RY-dependent manner. These results suggested that B3 domain-mediated (RY-mediated) transcriptional regulation of ABA-regulated genes is the ancestral function of ABI3/VP1; later ABI3/VP1 has acquired B3-independent (ABRE-dependent) transcriptional regulation during evolution of land plants, possibly by acquisition of a novel interaction with bZIPs that is strong enough to be independent from the RY element.…”

Section: Molecular Dissection Of Aba Signaling In the Model Bryophytementioning

confidence: 99%

ABA in bryophytes: how a universal growth regulator in life became a plant hormone?

2011

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Abscisic acid (ABA) is not a plant-specific compound but one found in organisms across kingdoms from bacteria to animals, suggesting that it is a ubiquitous and versatile substance that can modulate physiological functions of various organisms. Recent studies have shown that plants developed an elegant system for ABA sensing and early signal transduction mechanisms to modulate responses to environmental stresses for survival in terrestrial conditions. ABA-induced increase in stress tolerance has been reported not only in vascular plants but also in non-vascular bryophytes. Since bryophytes are the key group of organisms in the context of plant evolution, clarification of their ABA-dependent processes is important for understanding evolutionary adaptation of land plants. Molecular approaches using Physcomitrella patens have revealed that ABA plays a role in dehydration stress tolerance in mosses, which comprise a major group of bryophytes. Furthermore, we recently reported that signaling machinery for ABA responses is also conserved in liverworts, representing the most basal members of extant land plant lineage. Conservation of the mechanism for ABA sensing and responses in angiosperms and basal land plants suggests that acquisition of this mechanism for stress tolerance in vegetative tissues was one of the critical evolutionary events for adaptation to the land. This review describes the role of ABA in basal land plants as well as non-land plant organisms and further elaborates on recent progress in molecular studies of model bryophytes by comparative and functional genomic approaches.

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Section: Molecular Dissection Of Aba Signaling In the Model Bryophytementioning

confidence: 99%

ABA in bryophytes: how a universal growth regulator in life became a plant hormone?

2011

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“…Previous studies have shown that the activity of ABI3 increases dramatically in the presence of ABA (Parcy et al, 1994;Ezcurra et al, 2000;Suzuki et al, 2003;Marella et al, 2006;Nakashima et al, 2006;Kotak et al, 2007;Sakata et al, 2010;Park et al, 2011). Thus, the increased ABA level observed at high temperature is likely to potentiate the activity of ABI3 as a transcriptional regulator.…”

Section: Abi3 Abi5 and Dellas Mediate Aba And Ga Signaling To Activmentioning

confidence: 99%

ABA-INSENSITIVE3, ABA-INSENSITIVE5, and DELLAs Interact to Activate the Expression ofSOMNUSand Other High-Temperature-Inducible Genes in Imbibed Seeds inArabidopsis

Lim

Park²,

Lee³

et al. 2013

The Plant Cell

213

174

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Seeds monitor the environment to germinate at the proper time, but different species respond differently to environmental conditions, particularly light and temperature. In Arabidopsis thaliana, light promotes germination but high temperature suppresses germination. We previously reported that light promotes germination by repressing SOMNUS (SOM). Here, we examined whether high temperature also regulates germination through SOM and found that high temperature activates SOM expression. Consistent with this, som mutants germinated more frequently than the wild type at high temperature. The induction of SOM mRNA at high temperature required abscisic acid (ABA) and gibberellic acid biosynthesis, and ABA-INSENSITIVE3 (ABI3), ABI5, and DELLAs positively regulated SOM expression. Chromatin immunoprecipitation assays indicated that ABI3, ABI5, and DELLAs all target the SOM promoter. At the protein level, ABI3, ABI5, and DELLAs all interact with each other, suggesting that they form a complex on the SOM promoter to activate SOM expression at high temperature. We found that high-temperature-inducible genes frequently have RY motifs and ABA-responsive elements in their promoters, some of which are targeted by ABI3, ABI5, and DELLAs in vivo. Taken together, our data indicate that ABI3, ABI5, and DELLAs mediate high-temperature signaling to activate the expression of SOM and other high-temperature-inducible genes, thereby inhibiting seed germination.

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“…Indeed, the ABAsignaling pathway was reported to be present in P. patens, based on the activation of the wheat Em promoter and the PpLEA1 (the P. patens homolog of Em) promoter by exogenous ABA treatment (Knight et al, 1995;Kamisugi & Cuming, 2005) and its activation is performed through an ACGT-core ABRE (Kamisugi & Cuming, 2005;Sakata et al, 2010). PpABI3A was shown to activate synergistically the PpLEA1 promoter in concert with ABA (Marella et al, 2006;Khandelwal et al, 2010) and to partially complement the phenotypes of the Arabidopsis abi3-6 mutant such as ABA insensitivity of germination, the color of seeds, and the expression of seed maturation genes (MAT), but it failed to restore the expression of the LEA genes through the ACGT-core ABRE (Marella et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

ABSCISIC ACID INSENSITIVE3 regulates abscisic acid‐responsive gene expression with the nuclear factor Y complex through the ACTT‐core element in Physcomitrella patens

et al. 2013

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Summary The phytohormone ABA and the transcription factor ABSCISIC ACID INSENSITIVE 3 (ABI3)/VIVIPAROUS 1 (VP1) function in protecting embryos during the desiccation stage of seed development. In a similar signaling pathway, vegetative tissue of the moss Physcomitrella patens survives desiccation by activating downstream genes (e.g. LEA1) in response to ABA and ABI3. We show that the PpLEA1 promoter responds to PpABI3 primarily through the ACTT‐core element (5′‐TCCACTTGTC‐3′), while the ACGT‐core ABA‐responsive element (ABRE) appears to respond to ABA alone. We also found by yeast‐two‐hybrid screening that PpABI3A interacts with PpNF‐YC1, a subunit of CCAAT box binding factor (CBF)/nuclear factor Y (NF‐Y). PpNF‐YC1 increased the activation of the PpLEA1 promoter when incubated with PpABI3A, as did NF‐YB, NF‐YC, and ABI3 from Arabidopsis. This new response element (ACTT) is responsible for activating the ABI3‐dependent ABA response pathway cooperatively with the nuclear factor Y (NF‐Y) complex. These results further define the regulatory interactions at the transcriptional level for the expression of this network of genes required for drought/desiccation tolerance. This gene regulatory set is in large part conserved between vegetative tissue of bryophytes and seeds of angiosperms and will shed light on the evolution of this pathway in the green plant lineage.

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Regulation of the ABA-responsive Em promoter by ABI3 in the moss Physcomitrella patens

Cited by 42 publications

References 38 publications

ABA in bryophytes: how a universal growth regulator in life became a plant hormone?

ABA in bryophytes: how a universal growth regulator in life became a plant hormone?

ABA-INSENSITIVE3, ABA-INSENSITIVE5, and DELLAs Interact to Activate the Expression ofSOMNUSand Other High-Temperature-Inducible Genes in Imbibed Seeds inArabidopsis

ABSCISIC ACID INSENSITIVE3 regulates abscisic acid‐responsive gene expression with the nuclear factor Y complex through the ACTT‐core element in Physcomitrella patens

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