Regulation of the BCR signalosome by the class II peptide editor, H2-M, affects the development and repertoire of innate-like B cells

Ghosh, Debopam; Pham, Tho D.; Nanaware, Padma P.; Sengupta, Deepanwita; Adler, Lital N.; Li, Caiyun G.; Xiao, He; O’Mara, Mary E; Kantor, Aaron B.; Nguyen, Khoa D.; Yang, Yang; Eisenlohr, Laurence C.; Jensen, Peter E.; Herzenberg, Leonore A.; Stern, Lawrence J.; Boyd, Scott D.; Ghosn, Eliver; Mellins, Elizabeth

doi:10.1016/j.celrep.2021.110200

Cited by 3 publications

(2 citation statements)

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“…We speculate that p72 may have biological functions similar to those of IAV M1, regulating the activity of CMAS through interactions and affecting the synthesis of cell surface sialic acid receptors, thus participating in the adsorption process of the virus. CD63 is present in tetraspanin-enriched microdomains on the cell membrane, late endosomes, and lysosomes ( Hernaez et al, 2016 ; Ghosh et al, 2022 ). CD63 can enter the cell through caveolin-and clathrin-mediated endocytosis ( Pols and Klumperman, 2009 ), and ASFV can enter the cell through clathrin-mediated endocytosis ( Galindo et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Porcine alveolar macrophages host proteins interacting with African swine fever virus p72

Weng,

Zheng,

Liang

et al. 2024

Front. Microbiol.

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IntroductionAfrican swine fever virus (ASFV) is a highly contagious virus that spreads rapidly and has a mortality rate of up to 100% in domestic pigs, leading to significant economic losses in the pig industry. The major capsid protein p72 of ASFV plays a critical role in viral invasion and immune evasion.MethodsIn this study, we used yeast two-hybrid screening to identify host proteins interacting with p72 in porcine alveolar macrophages (PAMs) and verified these proteins using confocal microscopy and immunoprecipitation techniques.Results and DiscussionWe validated 13 proteins that interact with p72, including CD63, B2M, YTHDF2, FTH1, SHFL, CDK5RAP3, VIM, PELO, TIMP2, PHYH, C1QC, CMAS, and ERCC1. Enrichment analysis and protein-protein interaction network analysis of these interacting proteins revealed their involvement in virus attachment, invasion, replication, assembly, and immune regulation. These findings provide new insights into the function of p72 and valuable information for future research on the interaction between ASFV and host proteins.

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Section: Discussionmentioning

confidence: 99%

Porcine alveolar macrophages host proteins interacting with African swine fever virus p72

Weng,

Zheng,

Liang

et al. 2024

Front. Microbiol.

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“…Indeed, B-1 cell-BCRs are very similar ( Kantor et al, 1997 ; Vale et al, 2015 ) and most B-1 cells target similar self-antigens ( Tsuji et al, 2020 ). Thus, strong and intermediate BCR-signaling (i.e., via self-antigen recognition) on B-0 leads to the development of B-1 cells ( Hardy, 2006 ; Ghosh et al, 2022 ). While B-1 cells undergo this “positive selection,” B-2 cells follow the opposite trend and are derived from B-0s which are weakly self-reactive.…”

Section: The Development Of B-1 Cellsmentioning

confidence: 99%

B-1 cells in immunotoxicology: Mechanisms underlying their response to chemicals and particles

Hiéronimus

Huaux

2023

Front. Toxicol.

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Since their discovery nearly 40 years ago, B-1 cells have continued to challenge the boundaries between innate and adaptive immunity, as well as myeloid and lymphoid functions. This B-cell subset ensures early immunity in neonates before the development of conventional B (B-2) cells and respond to immune injuries throughout life. B-1 cells are multifaceted and serve as natural- and induced-antibody-producing cells, phagocytic cells, antigen-presenting cells, and anti-/pro-inflammatory cytokine-releasing cells. This review retraces the origin of B-1 cells and their different roles in homeostatic and infectious conditions before focusing on pollutants comprising contact-sensitivity-inducing chemicals, endocrine disruptors, aryl hydrocarbon receptor (AHR) ligands, and reactive particles.

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Post-transcriptional control of homeostatic B-cell signalling by HuR is required for innate B cell maintenance and function

Capitan-Sobrino,

Mouysset,

Maloudi

et al. 2024

Preprint

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Innate B-1 cells constitute a self-maintained layer of defence for early detection of bacteria, clearance of apoptotic cell debris and removal of autoantigens driving autoimmunity. B-1 cells are originated from foetal tissues but, as opposed to B-2 cells, the molecular mechanisms behind their development and homeostatic maintenance remain largely unknown. Here we demonstrate that post-transcriptional regulation by the RNA binding protein HuR is essential for the homeostatic self-replenishment of innate B-1 cells, the expansion of B-1 cell clones targeting self-antigens and the production of natural autoantibodies. HuR KO B-1 cells fail to express the high levels of surface BCR, TACI and BAFFR required for tonic signalling and cell survival. Mechanistically, HuR binds to the 3-UTRs of mRNAs encoding these surface receptors and of pro-survival molecules, like BCL-2 and MCL-1, promoting their translation into protein. In summary, we reveal the need of post-transcriptional regulation in BCR expression, tonic signalling and homeostatic maintenance of functional B-1 cells.

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Regulation of the BCR signalosome by the class II peptide editor, H2-M, affects the development and repertoire of innate-like B cells

Cited by 3 publications

References 67 publications

Porcine alveolar macrophages host proteins interacting with African swine fever virus p72

Porcine alveolar macrophages host proteins interacting with African swine fever virus p72

B-1 cells in immunotoxicology: Mechanisms underlying their response to chemicals and particles

Post-transcriptional control of homeostatic B-cell signalling by HuR is required for innate B cell maintenance and function

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