Regulation of the bovine oviductal fluid proteome

Abstract

Our objective was to investigate the regulation of the proteome in the bovine oviductal fluid according to the stage of the oestrous cycle, to the side relative to ovulation and to local concentrations of steroid hormones. Luminal fluid samples from both oviducts were collected at four stages of the oestrous cycle: pre-ovulatory (Pre-ov), post-ovulatory (Post-ov), and mid- and late luteal phases from adult cyclic cows (18-25 cows/stage). The proteomes were assessed by nanoLC-MS/MS and quantified by label-free method. Totally, 482 proteins were identified including a limited number of proteins specific to one stage or one side. Proportions of differentially abundant proteins fluctuated from 10 to 24% between sides at one stage and from 4 to 20% among stages in a given side of ovulation. In oviductal fluids ipsilateral to ovulation, Annexin A1 was the most abundant protein at Pre-ov compared with Post-ov while numerous heat shock proteins were more abundant at Post-ov compared with Pre-ov. Among differentially abundant proteins, seven tended to be correlated with intra-oviductal concentrations of progesterone. A wide range of biological processes was evidenced for differentially abundant proteins, of which metabolic and cellular processes were predominant. This work identifies numerous new candidate proteins potentially interacting with the oocyte, spermatozoa and embryo to modulate fertilization and early embryo development.

Designing 3-Dimensional In Vitro Oviduct Culture Systems to Study Mammalian Fertilization and Embryo Production

“…The oviduct is a convoluted tube consisting of longitudinal and circular muscular, and a stromal layer lined by a simple cuboidal to columnar epithelium containing both ciliated and secretory cells 83,106,123. The ciliated cells are important for gamete transport and sperm interaction, in particular helping to create a ‘sperm reservoir’; while the secretory cells are responsible for producing oviduct fluid; a mixture of specific cell secretions and serum transudate 1,2,10,63,118. In adult mammals (including the woman), four anatomical segments can be distinguished along the length of the oviduct; the infundibulum, ampulla, isthmus and utero-tubal junction, respectively11,122 (Fig.…”

Identification of Rabbit Oviductal Fluid Proteins Involved in Pre‐Fertilization Processes by Quantitative Proteomics

“…Changes in the abundance of diverse immunity proteins, such as CD109 and members of the S100 family, were also induced by insemination. CD109 is a cell surface glycoprotein and was also previously detected in the bovine ODF . CD109 can be processed by furin, and the soluble form is involved in TGF‐β signaling .…”

“…CD109 is a cell surface glycoprotein and was also previously detected in the bovine ODF. [21] CD109 can be processed by furin, and the soluble form is involved in TGF-β signaling. [73,74] Accordingly, this molecule might be involved in cell signaling events in the rabbit oviduct upon insemination.…”

“…More recently, an analysis based on MS has been conducted to characterize the complexity of the bovine ODF proteome [20] . Quantitative proteomics have further verified the hormonal regulation of bovine [21] and ovine ODF proteins [22] and have demonstrated gamete induced changes in the ODF proteome of pigs. [23] Furthermore, the effect of the presence of an embryo on the ODF protein profile has been demonstrated in mares.…”

Genes Encoding Mammalian Oviductal Proteins Involved in Fertilization are Subjected to Gene Death and Positive Selection

“…It has been demonstrated that the oviductal proteome changes with the different stages of the oestrous cycle (Lamy et al 2016; Soleilhavoup et al 2016), the presence of gametes (Georgiou et al 2005) or the presence of embryos (Smits et al 2017). The proteome of the oviduct epithelial cells has also been studied in humans (Wang et al 2016) and pigs (Seytanoglu et al 2008).…”