Regulation of the CCAAT-Binding NF-Y subunits in Arabidopsis thaliana

Gusmaroli, Giuliana; Tonelli, Chiara; Mantovani, Roberto

doi:10.1016/s0378-1119(01)00323-7

Cited by 134 publications

(144 citation statements)

References 30 publications

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“…These residues are conserved in hTAF II 80 and in the related PAF65␣. In NF-YB, the Asp-115 and Glu-98 residues corresponding to H2B Glu-90 and Glu-73 are among the relatively few amino acids present in all 26 NF-YB sequences from different species (65). We found that NF-Y is able to interact with an isoform of hTAF II 80-hTAF II 80␦, which lacks 10 amino acids in the ␣2 helix of the HFM (66), a fact that is mirrored by the interaction of hTAF II 80␦ with the H2B-like hTAF II 20.…”

Section: Discussionmentioning

confidence: 99%

NF-Y Recruitment of TFIID, Multiple Interactions with Histone Fold TAFIIs

Frontini¹,

Imbriano²,

DiSilvio³

et al. 2002

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

NF-Y Recruitment of TFIID, Multiple Interactions with Histone Fold TAFIIs

Frontini¹,

Imbriano²,

DiSilvio³

et al. 2002

Journal of Biological Chemistry

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“…The HAP complex consists of three subunits: HAP2/NF-YA/CBF-B, HAP3/NF-YB/CBF-A, and HAP5/NF-YC/CBF-C. Each subunit of the HAP complex contains a conserved domain that is responsible for DNA binding or protein-protein interaction. Although many genes encoding HAP subunits have been found so far (Edwards et al, 1998;Gusmaroli et al, 2001Gusmaroli et al, , 2002Thirumurugan et al, 2008), only a few of them have been studied (Lotan et al, 1998;Meinke, 1992;West et al, 1994;Kwong et al, 2003;Miyoshi et al, 2003). In this study, a rice HAP3 gene, DTH8, has been proven to play an important role in the regulation of flowering time, plant height, and yield potential.…”

Section: Possible Reason For the Pleiotropic Effects Of Dth8mentioning

confidence: 93%

“…In yeast (Saccharomyces cerevisiae) and mammals, each of the HAP subunits is encoded by a single gene (Mantovani, 1999), different from those in plants, in which all subunits are encoded by gene families (Gusmaroli et al, 2001(Gusmaroli et al, , 2002. The Arabidopsis genome encodes 10 AtNF-YA, 13 AtNF-YB, and 13 AtNF-YC subunits (Gusmaroli et al, 2002).…”

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confidence: 99%

DTH8 Suppresses Flowering in Rice, Influencing Plant Height and Yield Potential Simultaneously

et al. 2010

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The three most important agronomic traits of rice (Oryza sativa), yield, plant height, and flowering time, are controlled by many quantitative trait loci (QTLs). In this study, a newly identified QTL, DTH8 (QTL for days to heading on chromosome 8), was found to regulate these three traits in rice. Map-based cloning reveals that DTH8 encodes a putative HAP3 subunit of the CCAAT-box-binding transcription factor and the complementary experiment increased significantly days to heading, plant height, and number of grains per panicle in CSSL61 (a chromosome segment substitution line that carries the nonfunctional DTH8 allele) with the Asominori functional DTH8 allele under long-day conditions. DTH8 is expressed in most tissues and its protein is localized to the nucleus exclusively. The quantitative real-time PCR assay revealed that DTH8 could down-regulate the transcriptions of Ehd1 (for Early heading date1) and Hd3a (for Heading date3a; a rice ortholog of FLOWERING LOCUS T) under long-day conditions. Ehd1 and Hd3a can also be down-regulated by the photoperiodic flowering genes Ghd7 and Hd1 (a rice ortholog of CONSTANS). Meanwhile, the transcription of DTH8 has been proved to be independent of Ghd7 and Hd1, and the natural mutation of this gene caused weak photoperiod sensitivity and shorter plant height. Taken together, these data indicate that DTH8 probably plays an important role in the signal network of photoperiodic flowering as a novel suppressor as well as in the regulation of plant height and yield potential.

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“…For isolation of PhNF-YAs, RT-PCR was performed on P. hybrida first-strand cDNA with degenerate primers Ph-YF1 or Ph-YF2 derived from the C-terminal highly conserved region 15 and the miRBL MRE (Ph-YR; see Supplementary Table 2). For a phylogenetic tree constructed with these sequences and for the nomenclature, see Supplementary Figure 1.…”

Section: Methodsmentioning

confidence: 99%

“…miRNAs control gene expression by recognizing short complementary sequences in their transcripts (miRNA-recognition elements, or MREs), which are then post-transcriptionally targeted for cleavage or translational inhibition. Computational analysis of species with sequenced genomes and experimental evidence suggests that miR169 targets members of the NF-YA (also known as HAP2 or CBF-B) gene family 13,15 . We cloned and sequenced cDNAs encoding P. hybrida and snapdragon NF-YA family members (PhYA and AmYA cDNAs, respectively; Supplementary Fig.…”

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A conserved microRNA module exerts homeotic control over Petunia hybrida and Antirrhinum majus floral organ identity

et al. 2007

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It is commonly thought that deep phylogenetic conservation of plant microRNAs (miRNAs) and their targets 1,2 indicates conserved regulatory functions. We show that the blind (bl) mutant of Petunia hybrida 3 and the fistulata (fis) mutant of Antirrhinum majus 4,5 , which have similar homeotic phenotypes, are recessive alleles of two homologous miRNA-encoding genes. The BL and FIS genes control the spatial restriction of homeotic class C genes 6,7 to the inner floral whorls, but their ubiquitous early floral expression patterns are in contradiction with a potential role in patterning C gene expression. We provide genetic evidence for the unexpected function of the MIRFIS and MIRBL genes in the center of the flower and propose a dynamic mechanism underlying their regulatory role. Notably, Arabidopsis thaliana, a more distantly related species, also contains this miRNA module but does not seem to use it to confine early C gene expression to the center of the flower.The spatial partitioning of floral homeotic gene expression is crucial for wild-type flower development. Several transcription factors participate in this control, which aims at transcriptional silencing of the so-called 'C genes' outside their genuine expression domain in the inner two whorls of the flower, where they govern reproductive organ (stamen and carpel) development 6,7 . The functions of orthologous repressor genes, constituting the A function of the floral ABCs 6 , are, in part, comparable between different species 8 , as are some of the cis-acting regulatory regions within the large second intron of their structurally and functionally related target C genes AGAMOUS (AG) in Arabidopsis thaliana 7 , pMADS3 in P. hybrida 9 and PLENA and FARINELLI (PLE and FAR) in A. majus 10 . There are also exceptions to these overall similarities among species. For instance, orthologs of the A. thaliana APETALA2 (AP2) gene have no role in C gene regulation in P. hybrida 11 or A. majus 12 , raising the question of whether other genes fulfill this role. Candidates are the BL gene in P. hybrida and FIS in A. majus, which, when mutated, show markedly similar homeotically converted stamenoid petals in their second floral whorls 4,5 (Fig. 1).By a combination of transposon tagging and map-based cloning strategies, we cloned the BL and FIS genes and found that they encode homologous bona fide miRNAs (miRBL and miRFIS), related in their core sequences to members of the large miR169 family 13,14 (Fig. 2). The bl-1 and fis-1 alleles lie within large genomic deletions and thus represent null alleles; bl-2 and fis-2 are transposon induced and genetically unstable alleles (Fig. 2a). miRNA-encoding genes are relatively small targets for mutation, and therefore, recessive mutants are infrequent; bl and fis thus offer a rare opportunity to study and compare the function of potential orthologs in two plant species. miRNAs control gene expression by recognizing short complementary sequences in their transcripts (miRNA-recognition elements, or MREs), which are then post-transcrip...

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Regulation of the CCAAT-Binding NF-Y subunits in Arabidopsis thaliana

Cited by 134 publications

References 30 publications

NF-Y Recruitment of TFIID, Multiple Interactions with Histone Fold TAFIIs

NF-Y Recruitment of TFIID, Multiple Interactions with Histone Fold TAFIIs

DTH8 Suppresses Flowering in Rice, Influencing Plant Height and Yield Potential Simultaneously

A conserved microRNA module exerts homeotic control over Petunia hybrida and Antirrhinum majus floral organ identity

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