Regulation of the growth of nonmuscle heart cells in culture

Klein, Irwin; Daood, Monica J.

doi:10.1007/bf02620924

Cited by 14 publications

(2 citation statements)

References 14 publications

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“…The original medium was replaced with fresh medium at 18 and 24 h after plating. Cardiomyocytes were maintained under conditions designed to restrict the growth of nonmyo-cyte cells (26). Cells were incubated at 37°C in a water-saturated atmosphere of 95% air/5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Down-Regulation and Recovery of Endothelin-1 Binding and Signaling in Rat Cardiomyocytes

Harvey

Gandhi

Behal

et al. 2001

Biochemical and Biophysical Research Communications

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Section: Methodsmentioning

confidence: 99%

Down-Regulation and Recovery of Endothelin-1 Binding and Signaling in Rat Cardiomyocytes

Harvey

Gandhi

Behal

et al. 2001

Biochemical and Biophysical Research Communications

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“…Cardiac myocytes were prepared from neonatal rat ventricles using a modified Klein's method. 9 " 13 Hearts were removed from 1 -2-day-old neonatal Wistar male rats after decapitation. The ventricles from 20 hearts were minced into fine fragments with scissors in 0.025 M Hepes buffered minimum salt solution (MSS, Gibco, Grand Island, NY).…”

Section: Isolation Of Cardiac Myocytesmentioning

confidence: 99%

Modulation of the viability of immature cardiac myocytes by cardiac fibroblasts after hypothermic preservation—its values as a technique for evaluation of storage solutions

et al. 1995

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We evaluated the modulation of the viability of immature cardiac myocytes by cardiac fibroblasts after hypothermic preservation using three types of storage solutions—saline, University of Wisconsin solution, and MCDB 107 medium. Cardiac myocytes and fibroblasts were isolated from neonatal rat ventricles, and cultures of myocytes only or co-cultures with fibroblasts (myocyte: fibroblast 2:1) were established. On the fourth day of culture, the cultures were incubated at 4 °C for 6, 12, 18 and 24 hours in the different storage solutions. Enzymes were measured in the storage solutions immediately before and after hypothermic incubation. The cultures were then incubated for an additional 24 hours at 37 °C to evaluate the recovery of the myocyte beating rate. The myocyte beating rate in the co-culture groups showed significantly higher recovery ratios than the corresponding groups in which only myocytes were cultured. Complete recovery was observed in the group co-cultured in MCDB medium 24 hours after hypothermic incubation (83.4% of control—beating rate prior to hypothermic incubation) compared to the other co-cultured groups (15.4, 0%, respectively). Release of enzymes in the co-cultures was significantly suppressed compared to the cultured myocytes, and the greatest suppression was found after 24 hours of incubation in MCDB medium (CPK: 36.6 mIU/flask, LDH: 281.2 mIU/flask) compared to the other two co-cultured groups (CPK: 181.1, 281.1; LDH: 501.7, 773.2). Cardiac fibroblasts diminished myocytic injury after hypothermic preservation using various storage solutions, in which MCDB 107 medium showed the best overall protective effect. Thus, cardiac fibroblasts may play an important role in controlling myocytic viability under various hypothermic conditions.

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A cardiac myocyte culture system as an in vitro experimental model for the evaluation of hypothermic preservation

et al. 1993

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In cardiac transplantation, the donor heart is exposed to severe hypothermic and ischemic conditions. The purpose of the present study was to evaluate the functional and biochemical effects on cardiac myocytes cultured under hypothermic conditions. Cardiac myocytes were isolated from neonatal rat ventricles and cultured for 4 days, then incubated (1.5 x 10(6) myocytes/culture flask) for 24 h in media at 4, 10, 15, 20, and 37 degrees C. In addition, myocytes were incubated at 4 degrees C for 6, 12, 18, 24, 36, and 48 h. After each incubation, creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) were measured and the myocytes then cultured for an additional 24 h at 37 degrees C to evaluate the recovery of the myocyte beating rate. The recovery ratio of the myocyte beating rate following 24 h of varying temperature incubations was complete for the 10, 15, 20, and 37 degrees C groups, although it was markedly decreased in the 4 degrees C group, at 25.1% of the control; taken as the beating rate prior to hypothermic incubation. The release of CPK and LDH in the 4 degrees C group showed a three-fold increase compared to the other four groups, with a CPK of 147.2 mIU/flask and a LDH of 487.5 mIU/flask. The recovery of the beating rate for varying time incubations at 4 degrees C was complete for the 6- and 12-h groups, but decreased significantly in the other four groups, being 59.0% at 18 h, 28.2% at 24 h, 16.3% at 36 h, and 0% at 48 h.(ABSTRACT TRUNCATED AT 250 WORDS)

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Regulation of the growth of nonmuscle heart cells in culture

Cited by 14 publications

References 14 publications

Down-Regulation and Recovery of Endothelin-1 Binding and Signaling in Rat Cardiomyocytes

Down-Regulation and Recovery of Endothelin-1 Binding and Signaling in Rat Cardiomyocytes

Modulation of the viability of immature cardiac myocytes by cardiac fibroblasts after hypothermic preservation—its values as a technique for evaluation of storage solutions

A cardiac myocyte culture system as an in vitro experimental model for the evaluation of hypothermic preservation

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