Regulation of the HIF-system in human macrophages – Differential regulation of HIF-α subunits under sustained hypoxia

Potent Stimulation of Blood Flow in Fingers of Volunteers after Local Short‐Term Treatment with Low‐Frequency Magnetic Fields from a Novel Device

“…30 minutes after isolation and incubation of RNA from HUVECs, reverse transcription (RT) and real-time PCR were performed as extensively described by Poitz et al [7] using the following primers for real-time PCR: 5′-GATGGTGACTTTGGCTAGCTGG-3′ eNOS-forward (human nitric oxide synthase 3), 5′-TCCTGGAGGATGTGGCTGTC-3′ eNOS-reverse (human nitric oxide synthase 3),  5′-CATGAGCAAAGGCGCAGAAC-3′ iNOS-forward (human nitric oxide synthase 2), 5′-CCTGGCAATGGAGAGAAACTG-3′ iNOS-reverse (human nitric oxide synthase 2), 5′-TTGCGACCTTGACCATCTTTG-3′ HPRT1-forward (hyperparathyroidism 1), 5′-CTTTGCTGACCTGCTGGATTAC-3′ HPRT1-reverse (hyperparathyroidism 1).…”

Increased myeloid cell hypoxia-inducible factor-1 delays obliterative airway disease in the mouse

“…Previous studies showed that macrophages and neutrophils are able to express both HIF-1α and HIF-2α. 34,35 However, because the OAD development was significantly accelerated in mHIF-1α -/-recipients, one may assume that HIF-1 activity was the major contributor to the decreased OAD development in mVHL -/-recipients. In addition, Thompson et al 35 reported that HIF-2α regulates mainly key neutrophil functions, but we did not detect any significant difference in the number of MPO þ neutrophils between the groups at 3 or 10 days in our study.…”

TNF-α-mediated adhesion of monocytes to endothelial cells—The role of ephrinA1

“…The mutated and constitutively-active IκB-mutant was constructed as previously described [25]. Human ephrinA1 was amplified from oligo-dT reverse transcribed cDNA from HUVECs using the primers listed below that contain NheI or KpnI restriction site (underlined sequences), respectively, at the 5′ end and were cloned into pShuttle vector as described previously [25,26]. Primers used for amplification of the human ephrinA1 gene: Forward: CCGCTAGC-CTATGGAGTTCCTCTGG Reverse: CCGGTACC-TGTG GCATACACCTTCA.…”