Regulation of the NADP-glutamate dehydrogenase gene gdhA in Aspergillus nidulans by the Zn(II)2Cys6 transcription factor LeuB

Downes, Damien J.; Davis, Meryl A.; Kreutzberger, Sara D.; Taig, Brendan L.; Todd, Richard B.

doi:10.1099/mic.0.071514-0

Cited by 28 publications

(29 citation statements)

References 98 publications

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“…In A. flavus , the Zn(II) 2 Cys 6 zinc cluster‐encoding gene family is involved in asexual conidiation, sexual homothallic development and secondary metabolites (Chang and Ehrlich, ). In A. nidulans , TamA interacts with LeuB and regulates leucine biosynthesis, NADP‐glutamate dehydrogenase activity and nitrogen metabolism through the regulation of the gdhA gene encoding NADP‐glutamate dehydrogenase (Downes et al ., ). In A. oryzae , ManR, a novel Zn(II) 2 Cys 6 transcriptional activator, controls the β‐mannan utilization system (Ogawa et al ., ).…”

Section: Discussionmentioning

confidence: 97%

The fungal‐specific transcription factor Vdpf influences conidia production, melanized microsclerotia formation and pathogenicity in Verticillium dahliae

Luo

Mao

Wei

et al. 2016

Molecular Plant Pathology

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Verticillium dahliae is a soil-borne, hemibiotrophic phytopathogenic fungus that causes wilting in crop plants. Here, we constructed a random insertional mutant library using Agrobacterium tumefaciens-mediated transformation to study the pathogenicity and regulatory mechanisms of V. dahliae. The fungal-specific transcription factor-encoding gene Vdpf was shown to be associated with vegetative growth and virulence, with the highest transcript expression occurring during conidia formation in the V991 strain. The deletion mutants (ΔVdpf) and insertion mutants (IMΔVdpf) produced fewer conidia than did the wild-type (WT) fungi, which contributed to the reduced virulence. Unlike the WT, the complemented strains and IMΔVdpf, ΔVdpf formed swollen, thick-walled and hyaline mycelium rather than melanized microsclerotia. The ΔVdpf mutants were melanin deficient, with undetectable expression of melanin biosynthesis-related genes (Brn1, Brn2 and Scd1). The melanin deficiency was related to cyclic adenosine monophosphate (cAMP) and the G-protein-coupled signalling pathways in this study. Similar to the WT and complemented strains, the ΔVdpf and IMΔVdpf mutants could also successfully penetrate into cotton and tobacco roots, but displayed reduced virulence because of lower biomass in the plant roots and significantly reduced expression of pathogenicity-related genes in V. dahliae. In conclusion, these results provide insights into the role of Vdpf in melanized microsclerotia formation, conidia production and pathogenicity.

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Section: Discussionmentioning

confidence: 97%

The fungal‐specific transcription factor Vdpf influences conidia production, melanized microsclerotia formation and pathogenicity in Verticillium dahliae

Luo

Mao

Wei

et al. 2016

Molecular Plant Pathology

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“…nidulans crosses and genetic analysis were performed as described previously (48). A. nidulans transformations were conducted as described previously (49). Growth tests were performed as described in reference 48 at 37°C using Aspergillus nitrogen-free minimal medium (ANM) with nitrogen sources added at 10 mM (50).…”

Section: A Nidulans Genetic Manipulations and Growth Testsmentioning

confidence: 99%

Multiple Nuclear Localization Signals Mediate Nuclear Localization of the GATA Transcription Factor AreA

et al. 2014

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“…The first motif is consistent with consensus Zn(II)2Cys6 recognition sites containing two CCG triplets and is the more probable recognition motif, however variation in triplet sequence is known (Todd et al ., ; Badis et al ., ). We have recently shown that LeuB regulates gdhA in response to leucine levels via two sites of action, one of which corresponds to the TamA site (Downes et al ., ). This site does not conform to a LeuB consensus DNA binding site and therefore the action of LeuB via this sequence may be indirect.…”

Section: Discussionmentioning

confidence: 97%

“…Construction of gdhA reporter genes and strains used the strategy described previously (Downes et al ., ). Fragments of the gdhA promoter were amplified by PCR from MH1 genomic DNA using the forward primers gdhA1XhoI‐F, gdhA5XhoI‐F or gdhA8XhoI‐F with gdhA2BglII‐R.…”

Section: Methodsmentioning

confidence: 97%

“…GDH1 is also regulated by the branched-chain amino acid biosynthesis regulator Leu3p (Hu et al, 1995) and the amino acid starvation regulator Gcn4p (Riego et al, 2002). In A. nidu-lans, gdhA expression is regulated at the transcriptional level (Pateman, 1969;Hawkins et al, 1989;Caddick et al, 2006;Downes et al, 2013) by the branched amino acid biosynthesis regulator LeuB (Polotnianka et al, 2004;Downes et al, 2013) and the global nitrogen regulator AreA (Christensen et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Dual DNA binding and coactivator functions of Aspergillus nidulans TamA, a Zn(II)2Cys6 transcription factor

Downes

Davis

Wong

et al. 2014

Molecular Microbiology

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SummaryTranscription factors containing DNA binding domains generally regulate transcription by direct interaction with DNA. For most transcription factors, including the fungal Zn(II)2Cys6 zinc binuclear cluster transcription factors, the DNA binding motif is essential for function. However, Aspergillus nidulans TamA and the related Saccharomyces cerevisiae Dal81p protein contain Zn(II)2Cys6 motifs shown to be dispensable for function. TamA acts at several promoters as a coactivator of the global nitrogen GATA transcription factor AreA. We now show that TamA is the major transcriptional activator of gdhA, encoding the key nitrogen metabolism enzyme NADP-glutamate dehydrogenase. Moreover, activation of gdhA by TamA occurs primarily by a mechanism requiring the TamA DNA binding motif. We show that the TamA DNA binding motif is required for DNA binding of FLAGepitope-tagged TamA to the gdhA promoter. We identify a conserved promoter element required for TamA activation, and show that TamA and AreA are reciprocally required for full binding at the gdhA promoter under conditions where AreA is inactive at most promoters but active at gdhA. Therefore TamA has dual functions as a DNA-binding transcription factor and a non-DNA-binding coactivator. Dual DNA-binding and coactivator functions provide an additional level of combinatorial control to mediate gene-specific expression.

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Regulation of the NADP-glutamate dehydrogenase gene gdhA in Aspergillus nidulans by the Zn(II)2Cys6 transcription factor LeuB

Cited by 28 publications

References 98 publications

The fungal‐specific transcription factor Vdpf influences conidia production, melanized microsclerotia formation and pathogenicity in Verticillium dahliae

The fungal‐specific transcription factor Vdpf influences conidia production, melanized microsclerotia formation and pathogenicity in Verticillium dahliae

Multiple Nuclear Localization Signals Mediate Nuclear Localization of the GATA Transcription Factor AreA

Dual DNA binding and coactivator functions of Aspergillus nidulans TamA, a Zn(II)2Cys6 transcription factor

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