Regulation of the T15 idiotype‐positive, T‐independent immune response to <i>Streptococcus pneumoniae</i> R36 a(Pn). I. Specific suppressor T cells induced by Pn <i>in vitro</i> are antigen‐specific and idiotype‐positive

Černý, Jan; Caulfield, Michael J.; Siat, Maureen

doi:10.1002/eji.1830100902

Cited by 7 publications

(10 citation statements)

References 43 publications

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“…Evidence from a number of laboratories indicates that the maintenance of T15 antibodies as the dominant anti-PC idiotype is under strict regulation. Effector mechanisms involving helper T cells, suppressor T cells, and auto-anti-idiotype have all been shown to be able to effect idiotype regulation in this system (23)(24)(25)(26)(27). Our results, demonstrating better antipneumococcal protection with T 15 antibodies than with anti-PC antibodies of other idiotypes, suggest that the mechanisms regulating idiotype expression may play important roles in the maintenance of antibody populations that are optimally protective against various infectious agents.…”

Section: )/)mentioning

confidence: 57%

Anti-phosphorylcholine antibodies of the T15 idiotype are optimally protective against Streptococcus pneumoniae.

Briles¹,

Forman²,

Hudak³

et al. 1982

The Journal of Experimental Medicine

283

188

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In the mouse, most anti-PC antibody is found in one of the three murine anti-PC idiotype families: T15, M603, or M511. The antibodies within each of these idiotypic families have characteristic fine specificities for phosphorylcholine (PC)-analogues. In this paper we compare the ability of hybridoma IgM anti-PC antibodies of the three idiotype families to protect mice from fatal infection with S. pneumoniae. Antibody bearing the T15 idiotype was approximately 8 times as effective as antibody with the M603 idiotype and approximately 30 times as protective as antibody with the M511 idiotype. Reports by others have shown that the heavy chains of virtually all mouse anti-PC antibodies are produced by translocation of a single variable region gene and that the direct translation of this gene (in the absence of somatic mutations) results in heavy chains characteristic of the T15 idiotype. Thus, our findings suggest that the T15 germ line heavy chain variable region gene may have been selected through evolution to code for antibody binding PC-containing pathogens such as S. pneumoniae. Our observations may also explain the existence of regulatory mechanisms that result in maintenance of T15 idiotype expression in murine anti-PC immune responses.

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Section: )/)mentioning

confidence: 57%

Anti-phosphorylcholine antibodies of the T15 idiotype are optimally protective against Streptococcus pneumoniae.

Briles¹,

Forman²,

Hudak³

et al. 1982

The Journal of Experimental Medicine

283

188

View full text Add to dashboard Cite

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“…Most likely these suppressor cells are induced by interaction of T cells with bacterial antigens, i. e. they are antigen-specific. That antigen-specific T suppressor cells can arise during in vitro induction of a TI immune response has been recently described [38]. However, one can not exclude the possibility that they might be specific for the idiotpyes of B cells reactive with TI antigens.…”

Section: Discussionmentioning

confidence: 99%

Immune response against the T‐independent antigen α(1→3)dextran. I. Demonstration of an unexpected IgG response of athymic and germ‐free‐raised euthymic BALB/c mice

et al. 1982

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The primary antibody response in BALB/c mice to the T-independent bacterial antigen dextran B1355S [alpha(1 leads to 3)dextran] (Dex) was studied by means of isoelectric focusing, hemagglutination and immunodiffusion techniques. In response to a single immunization with 10 micrograms Dex all mice produce specific IgM antibodies. In addition, about 30% of conventionally raised BALB/c and BALB/c nu/ + mice, but 95% of germ-free (GF)-raised normal BALB/c and 100% of athymic BALB/c nu/nu mice produce specific IgG class anti-Dex antibodies. These antibodies include all IgG subclasses, carry predominantly the lambda light chain and the cross-reactive J558 idiotype and are specific for the alpha(1 leads to 3)glucosidic linkage. As compared to athymic and GF-raised mice, conventionally raised mice exhibit only a weak IgG response. The pronounced IgG production of GF-raised mice was not altered when adult mice were removed from their GF environment and housed under conventional conditions for several weeks prior to immunization with Dex. Reconstitution with isolated splenic T cells from conventionally raised, unprimed BALB/c mice reduces the remarkable capacity of BALB/c nu/nu mice to produce IgG anti-Dex antibodies. These findings suggest that the reduced capacity of conventionally raised BALB/c mice to mount an IgG response to the T-independent antigen Dex is due to a T cell-mediated suppressive mechanism which is neonatally induced by contact with environmental, i.e. bacterial, antigens.

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“…Based on these results it has become possible to investigate the idiotypic pattern of Pn-specific T, in BALB/c strain that have been previously shown to express a crossreactive T15 marker detected by conventional antisera (5). Using a functional assay of inhibition of"Is activity by monoclonal antibodies in vitro, we show herein that the idiogram of Pn-educated Ts overlaps with that of Pn-reactive B cells, that is, the Ts express most, but not all, known Id of the T15 family.…”

mentioning

confidence: 99%

Immunoglobulin idiotopes expressed by T cells. I. Expression of distinct idiotopes detected by monoclonal antibodies on antigen-specific suppressor T cells.

Černý¹,

Heusser²,

Wallich³

et al. 1982

The Journal of Experimental Medicine

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The idiotopic repertoire expressed by antigen-specific suppressor T cells (Ts) generated by Streptococcus pneumoniae strain R36a (Pn) in BALB/c strain mice was investigated using a panel of five monoclonal anti-idiotopic antibodies against TEPC-15/HOPC-8 myeloma proteins. Previous studies suggested that the anti-idiotopic antibodies recognize distinct idiotopic determinants within the T15 idiotype, and that Pn-reactive B cells express all of those idiotopes as shown by a specific inhibitory effect of the anti-idiotopic antibodies on induction of anti-Pn response in vitro as well as on the mature antibody plaque-forming cells. In this study we asked the question of whether anti-idiotopic (Id) can block the inductive and/or effector phases of generation of Ts which act on the Pn-reactive B cells. The presence of anti-Id during the activation of T cells with Pn did not prevent the generation of Ts. However, suppression mediated by Ts on responder lymphocytes (cultures of spleen cells or B cels) was inhibited (reversed) by four out of five anti-Id. Some of the antibodies recognize hapten (phosphorylcholine)-inhibitable Id in the paratope of Ig whereas others are directed against nonparatopic Id. These data indicate that the antigen receptor on Ts includes VH sequences both within and without the immunoglobulin in paratope, and that the Id repertoir of Ts overlaps with that of B cells.

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Regulation of the T15 idiotype‐positive, T‐independent immune response to Streptococcus pneumoniae R36 a(Pn). I. Specific suppressor T cells induced by Pn in vitro are antigen‐specific and idiotype‐positive

Cited by 7 publications

References 43 publications

Anti-phosphorylcholine antibodies of the T15 idiotype are optimally protective against Streptococcus pneumoniae.

Anti-phosphorylcholine antibodies of the T15 idiotype are optimally protective against Streptococcus pneumoniae.

Immune response against the T‐independent antigen α(1→3)dextran. I. Demonstration of an unexpected IgG response of athymic and germ‐free‐raised euthymic BALB/c mice

Immunoglobulin idiotopes expressed by T cells. I. Expression of distinct idiotopes detected by monoclonal antibodies on antigen-specific suppressor T cells.

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