Regulation of the Uptake of Sulfate by Chinese Cabbage (Brassica pekinensis) at Various Sulfate Concentrations in the Root Environment

Koralewska, A.; Posthumus, Freek; Stuiver, C.E.E.; Shahbaz, Muhammad; Stulen, I.; Kok, L.J. De

doi:10.1007/978-94-007-4450-9_7

Cited by 3 publications

(1 citation statement)

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“…The uptake of sulfate by the root is adjusted to the sulfur demand for growth, even at an external sulfate concentrations close to the K m value of the high affinity sulfate transporters (approx. 5 μM; Koralewska et al 2007Koralewska et al , 2012. The interaction between atmospheric H 2 S and pedospheric sulfate nutrition of plants has been extensively studied during the last three decades (De Kok 1990;De Kok et al 2000, 2002.…”

Section: -S -S/+s -S/cysmentioning

confidence: 99%

The Sulfur Pathway and Diagnosis of Sulfate Depletion in Grapevine

Tavares

Amâncio

2017

Proceedings of the International Plant Sulfur Workshop

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Sulfur is an essential nutrient to all plant species. Plants assimilate sulfur in a well-described pathway, which has been taken up by roots. Regulatory mechanism has been the subject of many research papers. However, recent studies highlighted differences between crop plants and the model plant Arabidopsis thaliana. Our work focuses on the identification of genes involved in the sulfur metabolism in the Vitis vinifera genome, and their response to sulfur deficiency and other abiotic stress endured by grapevine in the field, namely water stress. Here, we describe the identification and brief characterization of the first assimilation enzymes involved in the sulfur pathway, the enzyme responsible for sulfur activation, ATP sulfurylase (ATPS), and the two enzymes that reduce sulfate to sulfide, Adenosine 5 0-phosphosulate reductase (APR) and Sulfite reductase (SiR). A reduction was observed in the number of ATPS and APR isoforms identified in V. vinifera genome when compared to A. thaliana or Glycine max genomes. Two ATPS isoforms were present in the Vitis genome, of which only ATPS1 transcript was detected in the tested tissues, and one APR isoform, suggesting an absence of redundancy in the role of both enzymes. ATPS1, APR and SiR transcript level was up-regulated in response to 2 days exposure to sulfur deficiency in V. vinifera cell cultures, which was completely reversed by the addition of GSH to the culture medium. Apparently, oxidative stress triggered GSH has a pivotal role in the regulation of ATPS1, APR and SiR transcription level, since their up-regulation was observed in mRNA from field grapevine berries under water stress, which is known to induce oxidative stress. Grapevine (Vitis vinifera L.) is one of the most important crops worldwide for winemaking and also for table grapes. Grapevines can be successfully grown in a range of different climates and management conditions. Global climate changes are associated with water deficit and high evapotranspiration rates that can affect berry development, yield, and wine quality (Hannah et al. 2013).

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Section: -S -S/+s -S/cysmentioning

confidence: 99%

The Sulfur Pathway and Diagnosis of Sulfate Depletion in Grapevine

Tavares

Amâncio

2017

Proceedings of the International Plant Sulfur Workshop

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Zinc exposure has differential effects on uptake and metabolism of sulfur and nitrogen in Chinese cabbage

Stuiver

Posthumus

Parmar

et al. 2014

J. Plant Nutr. Soil Sci.

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Zinc (Zn) is a plant nutrient; however, at elevated levels it rapidly becomes phytotoxic. In order to obtain insight into the physiological background of its toxicity, the impact of elevated Zn2+ concentrations (1 to 10 μM) in the root environment on physiological functioning of Chinese cabbage was studied. Exposure of Chinese cabbage (Brassica pekinensis) to elevated Zn2+ concentrations (≥ 5 μM) in the root environment resulted in leaf chlorosis and decreased biomass production. The Zn concentrations of the root and shoot increased with the Zn2+ concentration up to 68‐fold and 14‐fold, respectively, at 10 μM compared to the control. The concentrations of the other mineral nutrients of the shoot were hardly affected by elevated Zn2+ exposure, although in the root both the Cu and Fe concentrations were increased at ≥ 5 µM, whereas the Mn concentration was decreased and the Ca concentration strongly decreased at 10 µM Zn2+. The uptake and metabolism of sulfur and nitrogen were differentially affected at ≥ 5 µM Zn2+. Zn2+ exposure resulted in an increase of sulfate uptake and the activity of the sulfate transporters in the root, and in enhanced total sulfur concentration of the shoot, which could be ascribed partially to an accumulation of sulfate. Moreover, Zn2+ exposure resulted in an up to 6.5‐fold increase in water‐soluble non‐protein thiol (and cysteine) concentration of the root. However, nitrate uptake by the root and the nitrate and total nitrogen concentrations of the shoot were decreased upon Zn2+ exposure, demonstrating the absence of a mutual regulation of the uptake and metabolism of sulfur and nitrogen at toxic Zn levels. Evidently, elevated Zn2+ concentrations in the root environment did not only disturb the uptake, distribution and assimilation of sulfate, it also affected the uptake and metabolism of nitrate in Chinese cabbage.

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